- 询价
- Cell Signaling Technology
- 4732
- USA
- 2025年08月12日
- W, IP
- Rabbit
- H,M,R
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Max Antibody
- 抗原：
synthetic peptide corresponding to residues around Tyr115 of human Max
- 应用范围：
W, IP
- 宿主：
Rabbit
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 库存：
大量
- 适应物种：
H,M,R
- 供应商：
CST
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M R | Endogenous | 21 to 22 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Max Antibody detects endogenous levels of total Max protein. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues around Tyr115 of human Max. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from Jurkat (human), C6 (rat), and Raw (mouse) cell lines, using Max Antibody. IP
Immunoprecipitation of Jurkat cell lysates with Max Antibody or control rabbit IgG followed by Western blot analysis using Max Antibody. |
| Background | Members of the Myc/Max/Mad network function as transcriptional regulators with roles in various aspects of cell behavior including proliferation, differentiation and apoptosis (1). These proteins share a common basic-helix-loop-helix leucine zipper (bHLH-ZIP) motif required for dimerization and DNA-binding. Max was originally discovered based on its ability to associate with c-Myc and found to be required for the ability of Myc to bind DNA and activate transcription (2). Subsequently, Max has been viewed as a central component of the transcriptional network, forming homodimers as well as heterodimers with other members of the Myc and Mad families (1). The association between Max and either Myc or Mad can have opposing effects on transcriptional regulation and cell behavior (1). The Mad family consists of four related proteins; Mad1, Mad2 (Mxi1), Mad3 and Mad4, and the more distantly related members of the bHLH-ZIP family, Mnt and Mga. Like Myc, the Mad proteins are tightly regulated with short half-lives. In general, Mad family members interfere with Myc-mediated processes such as proliferation, transformation and prevention of apoptosis by inhibiting transcription (3,4). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验【求助】测序结果分析：应该对比的序列是看max score 最大的那个序列，还是max ident最接近的序列
shaoyibo 请问BLAST测序结果后，应该对比的序列是看max score 最大的那个序列，还是max ident最接近的序列?有人说是max score 最大的那个序列，可是我发现有好几个同样分值的，到底该如何看测序结果？请大侠指教，十万火急，多谢了 lmnsmu 我认为首先看max score最大的序列，同样分值的好几个，说明你blast的序列可能是个保守序列，在同一类或家族的基因均有。你可以点blast的条形
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Soybean (Glycine max) Transformation Using Immature Cotyledon Explants
Agrobacterium tumefaciens -mediated transformation of soybeans can be accomplished using immature zygotic cotyledons as target tissues providing an alternate explant to embryogenic tissue cultures, proliferating meristems, and cotyledonary
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