- 询价
- Cell Signaling Technology
- 3992
- USA
- 2025年08月10日
- W, IP, IHC-P
- Rabbit
- H,M,R,Mk,B
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Gα (pan) Antibody
- 抗原：
synthetic peptide corresponding to residues of human guanine nucleotide-binding protein Gα (q) subunit
- 应用范围：
W, IP, IHC-P
- 宿主：
Rabbit
- 库存：
大量
- 级别：
详见MSDS文件
- 供应商：
CST
- 适应物种：
H,M,R,Mk,B
- 保质期：
详见说明书
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IHC-P | H M R Mk B | Endogenous | 39, 41 | Rabbit |
| Protocols | |
|---|---|
| Specificity / Sensitivity | Gα (pan) Antibody detects total levels of endogenous G protein alpha subunits. The antibody may not detect G(12)-α, G(13)-α (13), G(s)-α (s) and G(olf)-α. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues of human guanine nucleotide-binding protein Gα (q) subunit. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from BAEC, HEK293, NIH/3T3, C6 and COS cells, using Gα (pan) Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Gα (pan) Antibody in the presence of control peptide (left) or antigen specific peptide (right). IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using Gα (pan) Antibody. |
| Background | Heterotrimeric guanine nucleotide-binding proteins (G proteins) consist of α, β and γ subunits and mediate the effects of hormones, neurotransmitters, chemokines and sensory stimuli. To date, over 20 known Gα subunits have been classified into four families, Gα(s), Gα(i/o), Gα(q) and Gα(12), based on structural and functional similarities (1,2). Phosphorylation of Tyr356 of Gα(q)/Gα(11) is essential for activation of the G protein, since phenylalanine substitution for Tyr356 changes the interaction of Gα with receptors and abolishes ligand-induced IP3 formation (3). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
Protein A, derived from the cell wall of Staphylococcus aureus , and protein G, derived from the cell wall of certain strains of b -hemolytic Streptococcci , binds the Fc region
Antibody Purification: Affinity Chromatography Protein A and Protein G Sepharose
G coupled to agarose. In the Subheading 3 there are also protocols for affinity purification using a specific ligand coupled to commercial matrices like CNBr- Sepharose 4-B and Affigel.
Analysis and Purification of Antibody Fragments Using Protein A, Protein G, and Protein L
Today, monoclonal antibodies (mAbs) form the largest category of biopharmaceuticals in clinical trials and their number is expanding rapidly (DataMonitor 2007). The antibodies or functional antibody fragments are being produced in artificial
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