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文献和实验Genome‐Wide Location Analysis by Pull Down of In Vivo Biotinylated Transcription Factors
the transcriptional regulator of interest with a short bio epitope which is specifically biotinylated by the E. coli enzyme BirA. The biotinylated transcription factor can then be selectively pulled down on streptavidin beads under stringent conditions. This unit
Peptide Microarrays for Serum Antibody Diagnostics
immobilization of peptide probes in a two-step procedure first by coupling of biotinylated peptides to hydrazide-modified streptavidin and then utilizing a subsequent chemoselective reaction between the hydrazide linkers of the streptavidin and an aldehyde-coated
Five-Step Process for Screening Antisense Compounds for Efficacy: Gene Target IL-12Rb2
with altered functional properties. The antisense compounds used here are phosphorodiamidate morpholino oligomers conjugated to an arginine-rich cell penetrating peptide (P-PMO). We describe a five-step process for selecting the best candidate antisense
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