- 询价
- Cell Signaling Technology
- 2570
- USA
- 2025年12月14日
- W
- Rabbit
- H,M
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Acetyl-p53 (Lys379) Antibody
- 抗原：
synthetic acetylated peptide corresponding to residues surrounding Lys379 of mouse p53
- 应用范围：
W
- 宿主：
Rabbit
- 适应物种：
H,M
- 保质期：
详见说明书
- 库存：
大量
- 供应商：
CST
- 级别：
详见MSDS文件
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M | Endogenous | 53 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Acetyl-p53 (Lys379) Antibody detects endogenous levels of p53 only when acetylated at Lys379 (mouse protein) or Lys382 (human protein). |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic acetylated peptide corresponding to residues surrounding Lys379 of mouse p53. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from NIH/3T3 (left) and MCF-7 (right) cells, untreated, doxorubicin-treated (0.5 μM, 24 hours) or doxorubicin and trichostatin A-treated (TSA, #9950, 400 nM, 24 hours), using Acetyl-p53 (Lys379) Antibody. |
| Background | The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro (2,3). DNA damage induces phosphorylation of p53 at Ser15 and Ser20 and leads to a reduced interaction between p53 and its negative regulator, the oncoprotein MDM2 (4). MDM2 inhibits p53 accumulation by targeting it for ubiquitination and proteasomal degradation (5,6). p53 can be phosphorylated by ATM, ATR, and DNA-PK at Ser15 and Ser37. Phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and activation of p53 in response to DNA damage (4,7). Chk2 and Chk1 can phosphorylate p53 at Ser20, enhancing its tetramerization, stability, and activity (8,9). p53 is phosphorylated at Ser392 in vivo (10,11) and by CAK in vitro (11). Phosphorylation of p53 at Ser392 is increased in human tumors (12) and has been reported to influence the growth suppressor function, DNA binding, and transcriptional activation of p53 (10,13,14). p53 is phosphorylated at Ser6 and Ser9 by CK1δ and CK1ε both in vitro and in vivo (13,15). Phosphorylation of p53 at Ser46 regulates the ability of p53 to induce apoptosis (16). Acetylation of p53 is mediated by p300 and CBP acetyltransferases. Inhibition of deacetylation suppressing MDM2 from recruiting HDAC1 complex by p19 (ARF) stabilizes p53. Acetylation appears to play a positive role in the accumulation of p53 protein in stress response (17). Following DNA damage, human p53 becomes acetylated at Lys382 (Lys379 in mouse) in vivo to enhance p53-DNA binding (18). Deacetylation of p53 occurs through interaction with the SIRT1 protein, a deacetylase that may be involved in cellular aging and the DNA damage response (19).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验【求助】P53的翻译后修饰都有哪些？ 怎样验证其修饰后与靶标启动子的结合活性的变化？
化，如Ser313、Ser314、Thr377、Ser378(被CHK1 ,CHK2磷酸化)、Ser366 (只被CHK2磷酸化)、Thr387 (只被CHK1磷酸化)。这些位点的磷酸化可能会改变 Lys373、Lys382乙酰化的方式，但不会改变Lys320，因此可以用来区分P300/CREB结合蛋白（CBP）和P300/CBP 相关因子(PCAF)活性[20].。 当大多数p53的磷酸化可以增强蛋白质的稳定性和活性时，一些位点的磷酸化（Thr55 、Thr155 、Ser205
人总P53蛋白(Total P53)ELISA试剂盒 说明书
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人总P53 蛋白 ( Total P53 )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 P53 单抗包被于酶标板上，标准品和样品中的 P53与单抗结合，加入生物素化的抗人 P53 ，形成免疫复合物连接在板上，辣根
豚鼠总P53蛋白(Total P53)ELISA试剂盒 说明书
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 豚鼠总P53 蛋白 ( Total P53 )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗豚鼠 P53 单抗包被于酶标板上，标准品和样品中的 P53与单抗结合，加入生物素化的抗豚鼠 P53 ，形成免疫复合物连接在板上，辣根
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