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文献和实验L- 羟脯氨酸 L-hydroxyproline(Pro-OH) (Hyp) 131.13 270d 36.11 5.83 (1)1.92 (2)9.73 DL- 异亮氨酸 DL-isoleucine(lle) 131.17 292d 2.229 6.02 (1)2.36(2)9.68 L- 异亮氨酸 L-isoleucine
Preparing a Selenomethionyl Protein
, phenylalanine to the culture. Add 50mg leucine, isoleucine, valine to the culture (all as solid powders). Add 120mg DL-Se-Met or 60mg L-Se-Met to the culture (as a solid powder). Continue to grow the culture for 15 minutes. Induce with 1mL
Preparing a Selenomethionyl Protein
and grow until an OD600 =0.5-0.6. (~2.0 - 2.5 hours) Add 100mg threonine, lysine hydrochloride, phenylalanine to the culture. Add 50mg leucine, isoleucine, valine to the culture (all as solid powders). Add 120mg DL-Se-Met or 60mg L-Se-Met
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