Myr-ZIP

Nat Metab：栾冰团队发现锌离子介导脂肪细胞与交感神经信号交流，为肥胖治疗提供新思路

细胞调控交感神经的机制，作者通过对体外条件下培养的产热细胞上清液进行质谱分析发现，产热细胞在 GPCR 信号刺激下分泌大量的锌离子。值得注意的是，锌离子能够促进体外培养的原代交感神经活性。综上表明，产热脂肪细胞可通过分泌锌离子促进交感神经的活性。   后续作者发现锌离子可通过交感神经细胞上的 ZIP12 通道进入到胞内并激活 ERK 信号通路从而促进交感神经突触生长。在肥胖小鼠的脂肪组织中，炎症因子诱导锌伴侣蛋白金属硫蛋白 2 表达上调，该蛋白通过锌离子螯合作用减少了产热脂肪细胞的锌分泌，抑制

Monitoring Protein Kinase A Activities Using Expressed Substrate in Live Cells

for monitoring PKA activity in live cells. A triparti substrate for PKA (Myr-HA-β2 AR-C) is constructed that contains an N-terminal myristylation sequence followed by an antigenic hemagglutinin epitope tag and a substrate motif (the C-terminal tail of human β2

Detection of Expression Patterns in Hydra Pattern Formation

and transcription factors to be present in this more than 500 Myr-year-old phylum, future work will focus on the function and expression of these genes in Hydra pattern formation and regeneration. This chapter presents an in situ hybridization protocol