- 询价
- Cell Signaling Technology
- 2233
- USA
- 2026年01月13日
- 免疫荧光（IF）,流式细胞（Flow Cyt）
- 小鼠
- 不限
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- 详细信息
- 文献和实验
- 技术资料
- 适应物种：
不限
- 应用范围：
免疫荧光（IF）,流式细胞（Flow Cyt）
- 抗体英文名：
Myc-Tag (9B11) Mouse mAb (Alexa Fluor 647 Conjugate)
- 是否单克隆：
1
- 保存条件：
-20°c
- 库存：
大量
- 级别：
详见MSDS文件
- 供应商：
CST
- 抗原：
/
- 抗原来源：
/
- 保质期：
详见说明书
- 宿主：
小鼠
- 规格：
100 ul (50 tests)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> /100 ul (50 tests)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥面议 | |
|---|---|---|---|
| 规格： | 100 ul (50 tests) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
| 规格： | 100 ul (50 tests) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - Complementary Reagents
Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 647 Conjugate) #2233
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IF-IC F | All | Transfected Only | Mouse IgG2a |
Applications Key: IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
Protocols
- 2233:
- Flow , Immunofluorescence
Specificity / Sensitivity
Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 647 Conjugate) detects transfected proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino or carboxy terminus of targeted proteins in transfected mammalian cells.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL). The antibody was conjugated to Alexa Fluor® 647 under optimal conditions with an F/P ratio of 2-6. The Alexa Fluor® 647 dye is maximally excited by red light (e.g. 633 nm He-Ne laser). Antibody conjugates of the Alexa Fluor® 647 dye produce bright far-red-fluorescence emission, with a peak at 665 nm.
Flow Cytometry
Flow cytometric analysis of COS cells, untransfected (blue) or transfected with Myc-tagged Akt (green), using Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 647 Conjugate).
IF-IC
Confocal immunofluorescent analysis of COS cells expressing a Myc-tagged protein (left) and untreated (right), using Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor). Actin filaments have been labeled with DY-554 phalloidin (red).
Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in cells transfected with Myc-tagged protein.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
- 2279 Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 488 Conjugate)
- 2350 HA-Tag (6E2) Mouse mAb (Alexa Fluor® 488 Conjugate)
- 2276 Myc-Tag (9B11) Mouse mAb
- 2367 HA-Tag (6E2) Mouse mAb
- 2985 Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate)
- 2365 His-Tag Antibody
- 2272 Myc-Tag Antibody
- 2372 β-Gal (14B7) Mouse mAb
- 2396 MBP (8G1) Mouse mAb
Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.
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文献和实验Antibody250µlIF-IC HCA4410SAnti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate)Secondary Antibody250µlIF-IC F4412SAnti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate)Secondary Antibody250µlIF-IC F4413SAnti-rabbit IgG (H+L
WHOLE MOUNT IN SITU HYBRIDIZATION. Mouse Embryos
4.5 (without removing prehybridization solution.) C. Repeat the addition of the 2XSSC wash twice more. Remove the mix and wash twice, 30 min each time, in 2XSSC pH7/0.1% CHAPS 70 °C. Wash twice, 10 min each, in Maleic Acid Buffer ( MAB; 100 mM maleic acid
：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
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