- 询价
- Cell Signaling Technology
- 9175
- USA
- 2026年05月28日
- W, IHC-P
- H,Mk
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- 文献和实验
- 技术资料
- 抗体英文名：
Stat1 (42H3) Rabbit mAb
- 抗原：
synthetic peptide corresponding to the sequence of human Stat1
- 应用范围：
W, IHC-P
- 适应物种：
H,Mk
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 库存：
大量
- 供应商：
CST
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: H=Human Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P | H Mk | Endogenous | 84, 91 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Stat1 (42H3) Rabbit mAb detects endogenous levels of total Stat1 protein independent of phosphorylation. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human Stat1. Western Blotting
Western blot analysis of extracts from HeLa and COS cells, using Stat1 (42H3) Rabbit mAb (Human Specific). IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human MALToma, showing cytoplasmic and nuclear staining, using Stat1 (42H3) Rabbit mAb (Human Specific). IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human Non-Hodgkin's lymphoma, using Stat1 (42H3) Rabbit mAb (Human Specific). IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Stat1 (42H3) Rabbit mAb (Human Specific) in the presence of control peptide (left) or Stat1 blocking peptide (9175 specific) #1079 (right). |
| Background | The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation. |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063. For Research Use Only. Not For Use In Diagnostic Procedures. |
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说明书推荐的反应体系进行 CUT&Tag 实验；其中，pG-Tn5 转座子使用的终浓度为 0.04μM ，一抗为 H3K27me3（CST，#9733），二抗为 Goat anti Rabbit（Bioworld，#BS13271）。 A、文库峰型检测 使用 Agilent 2100 Bioanalyzer 进行文库分布检测。 图 2. 使用 TD901 构建 100 cells CUT&Tag 文库峰型图 图 3. 使用 TD901 构建 10,000 cells CUT&Tag
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