- 询价
- Cell Signaling Technology
- 2544
- USA
- 2025年12月12日
- W
- H
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-FGF Receptor 1 (Tyr766) (1E5) Rabbit mAb
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Tyr766 of human FGF receptor-1
- 应用范围：
W
- 供应商：
CST
- 库存：
大量
- 保质期：
详见说明书
- 适应物种：
H
- 级别：
详见MSDS文件
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/300 ul (30 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | 300 ul (30 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H | Transfected Only | 120, 140 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-FGF Receptor 1 (Tyr766) (1E5) Rabbit mAb detects transfected levels of FGFR-1 only when phosphorylated at tyrosine 766. The antibody may cross-react with other FGFR family members and some activated protein tyrosine kinases including EGFR and insulin/IGF-I receptors. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr766 of human FGF receptor-1. Western Blotting
Western blot analysis of cell extracts from COS cells overexpressing human FGFR1 and control COS cells, using Phospho-FGF Receptor-1 (Tyr766) (1E5) Rabbit mAb. Before antibody probing, the transferred membrane was untreated (upper) or CIP treated (lower) to confirm the phospho-specificity of the antibody. |
| Background | Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through cell surface receptor tyrosine kinases. There are four members of the FGF receptor family: FGFR1 (flg), FGFR2 (bek, KGFR), FGFR3, and FGFR4. Each receptor contains an extracellular ligand binding domain, a transmembrane domain, and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR1 can be phosphorylated: Tyr463, 583, 585, 653, 654, 730, and 766. Tyr653 and Tyr654 are important for catalytic activity of activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCγ (4,5). Autophosphorylation of Tyr766 of FGFR1 is critical for phospholipase C (PLC) binding and activation and also plays a role in the negative regulation of FGFR1 activity in vivo (6).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063. For Research Use Only. Not For Use In Diagnostic Procedures. |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验h）即可。2. 修复大法——不仅仅是「煮一煮」微波炉修复：简单易行效果好，CST 推荐使用微波炉完成修复。合适的修复液：根据抗体说明书使用合适的修复液。用柠檬酸修复后，切片需浸泡在修复液中，自然冷却；而用 EDTA 修复后，切片可直接从修复缸中取出，直接进行下一步。注：使用不同的修复方式和不同生产商的抗体检测人肺癌组织中 EGFR 的表达。第一排为 CST 的 EGF Receptor (D38B1) XP® Rabbit mAb（#4267），EDTA 的修复方式明显优于柠檬酸盐及胃蛋白
。所以，我们建议使用微波炉或高压锅加热煮沸以达到最佳抗原修复效果。抗体：Phospho–Stat3 (Tyr705)(D3A7) XP. Rabbit mAb #9145样本：石蜡包埋人类肺部肿瘤样本煮沸设备：浴锅 (左)，微波炉 (中)，高压锅 (右)免疫染色—封闭在 IHC–P 中，我们建议在室温下在含有 Tween20 的 TBST 缓冲液和 5% 正常山羊血清 (NGS) 中封闭样品，时长 1 小时，以防止非特异性背景染色。在进行 IHC–F 时，在含有 0.3% Triton. X–100 的 1X
used in accordance withmanufacturer recommendationsSignalStain?DAB Substrate Kit #8059:IHC analysis of paraffin-embedded human breastcarcinoma using Phospho-Stat3 (Tyr705) (D3A7) XP? Rabbit mAb #9145. Chromogenicdetection was performed using #8059 (left
技术资料暂无技术资料 索取技术资料
