- 询价
- Cell Signaling Technology
- 2372
- USA
- 2025年08月13日
- W, IP, IF-IC, F
- All
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
β-Gal (14B7) Mouse mAb
- 抗原：
recombinant beta-galactosidase protein
- 应用范围：
W, IP, IF-IC, F
- 级别：
详见MSDS文件
- 供应商：
CST
- 适应物种：
All
- 库存：
大量
- 保质期：
详见说明书
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| W IP IF-IC F | All | Transfected Only | Mouse IgG1 |
| Protocols | |
|---|---|
| Specificity / Sensitivity | Beta-Gal 14B7 Mouse mAb detects transfected beta-galactosidase fusion proteins. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with recombinant beta-galactosidase protein. Western Blotting
Western blot analysis of cells expressing beta-galactosidase (lane 1) or cells without transfection (lane 2), using Beta-Gal (14B7) Mouse mAb. IP
Immunoprecipitation of cell extracts overexpressing beta-galactosidase (lane 1) or cells without transfection (lane 2), using Beta-Gal (14B7) Mouse mAb, followed by Western blot analysis using the same antibody. Flow Cytometry
Flow cytometric analysis of COS cells, untransfected (blue) or transfected with β-galactosidase (green), using β-Gal (14B7) Mouse mAb. IF-IC
Immunocytochemical staining of cells expressing b-galactosidase (left) or cells without transfection (right), using b-Gal 14B7 Mouse mAb. |
| Background | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. Eukaryotic genes can be cloned into E. coli beta-galactosidase (lacZ) gene (1-3). This results in the expression of a fusion protein containing the desired protein with a beta-gal tag. An antibody to this tag can then be used to isolate the fusion protein or detect its expression within cells or tissues.
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Staining Whole Mouse Embryos for β-Galactosidase (lacZ) Activity
) activity using X-gal. RELATED INFORMATION A protocol for Staining Frozen Mouse Embryo Sections for β-Galactosidase (lacZ) Activity is also available. MATERIALS Reagents Detergent rinse Embryo staining solution
Methodologies for Staining and Visualisation of β-Galactosidase in Mouse Embryos and Tissues
This chapter provides information on β-galactosidase staining of whole mouse embryos, organs, tissue sections, and cultured cells, as well as double staining with horseradish peroxidase and use as a tool for genotyping. Using these protocols
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