- 询价
- Cell Signaling Technology
- 2558
- USA
- 2025年12月13日
- W, IF-IC
- Rabbit
- H
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-p57 Kip2 (Thr310) Antibody
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Thr310 of human p57 Kip2
- 应用范围：
W, IF-IC
- 宿主：
Rabbit
- 供应商：
CST
- 库存：
大量
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 适应物种：
H
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-IC | H | Endogenous | 57 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-p57 Kip2 (Thr310) Antibody detects endogenous levels of p57 Kip2 only when phosphorylated at threonine 310. This antibody may cross-react with p27 Kip1 when phosphorylated at Thr187. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr310 of human p57 Kip2. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from untreated and dexamethasone-treated (50 nM, 16 h) HeLa cells, using Phospho-p57 Kip2 (Thr310) Antibody (upper) and p57 Kip2 Antibody #2557 (lower). Lysates were treated with lambda phosphatase NEB#P0753 (10,000 U/ml for 30 mins) to confirm the phospho-specificity of the phospho-antibody. IF-IC
Confocal immunofluorescent analysis of HeLa cells, dexamethasone-treated (left) or phosphatase-treated (right), labeled with Phospho-p57 Kip2 (Thr310) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). |
| Background | p27 Kip1 is a member of the Cip/Kip family of cyclin-dependent kinase inhibitors. Like its relatives, p57 Kip2 and p21 Waf1/Cip1, the ability to enforce the G1 restriction point is derived from its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes. Expression levels of p27 are upregulated in quiescent cells and in cells treated with cAMP or other negative cell cycle regulators. Downregulation of p27 can be induced by treatment with interleukin-2 or other mitogens; this involves phosphorylation of p27 and its degradation by the ubiquitin-proteasome pathway (1-4). Levels of p57 Kip2 are controlled by ubiquitination/degradation via the Skp1/Cul1/F-box-type E3 ubiquitin ligase complex SCF-Skp2, and this effect is dependent on Thr310 (5). A similar threonine phosphorylation site in p27 Kip1, Thr187, has also been shown to regulate protein stability (6).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
Absorption Control in Immunohistochemistry Using Phospho-Peptides Immobilized on Magnetic Beads
neutralization of phospho-specific antibodies with phospho-peptides immobilized on magnetic beads. This technique allows for sequestration of antibody–peptide complex from the incubation solution, minimizing the risk of formation of unblocked antibodies capable
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