研选同类产品更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 用户评价
- 文献和实验
- 技术资料
- 库存：
999
- 英文名：
Collagenase
- 保质期：
2-5年
- 供应商：
上海曼博生物医药科技有限公司
- 保存条件：
2°C~ 8 °C
- 规格：
1g（GMP级）
产品简述
Nordmark提供高质量胶原酶NB产品，特别适用于从各种组织中分离细胞。严格的生产标准带来可靠的批间一致性和优异的性能，旨在获得高产量的活细胞。
产品介绍
Nordmark致力于生产生物制药活性成分和药物产品，在胰酶和胶原酶的生产方面具有长达30年的历史经验。凭借这些专业知识，该公司已成功地将自己定位于国际市场。Nordmark的技术包括提取生物原材料（例如用于获得胰酶）、微生物发酵（例如用于分离干细胞的胶原酶）和从细胞培养物中提取活性成分（例如用于获得抗体）。
产品特点
- 超过30年的高质量胶原酶专业供应，按照制药级标准进行纯化和生产
- 组织细胞解离的整体解决方案：按照不同种属、组织/器官、细胞类型进行分类
- 不同规格，纯度，活性的胶原酶以及中性水解酶
- 从科研级到无动物源GMP级别多重选择，相关GMP产品已在FDA备案DMF
- 满足绝大多数组织/器官的单细胞解离需求，包括提供敏感细胞解离专用的温和解离酶方案
- 提供优化过的胰岛专用细胞解离方案和产品
- 用户使用友好：提供优化过的细胞解离Protocol
- 高性价比：相同酶活性条件下价格与全球主流品牌相比具有明显优势
- 各类应用的大量文献支持
应用领域
各种科研用途的器官，组织，细胞的解离；具有GMP无动物源等级，可进行临床用途的细胞解离。
按种属划分
| 人 | 猪 | 啮齿动物 | 其他 |
按照组织划分
| 脂肪 | 肝脏 | 皮肤 |
| 骨 | 肺 | 甲状腺 |
| 软骨 | 肌肉 | 肿瘤 |
| 结肠 | 神经 | 脐带 |
| 胃/肠 | 胰腺 | 心脏 |
| 生殖系统 |
按照细胞种类划分
| 腺泡细胞 | 上皮细胞 | 肌肉细胞 |
| 脂肪细胞 | 成纤维细胞 | 神经元 |
| 羊膜细胞 | 肝细胞 | 卵母细胞 |
| 心肌细胞 | 胰岛 | 成骨细胞 |
| 软骨细胞 | 白细胞 | 雪旺细胞 |
| 绒毛膜细胞 | 肺细胞 | 干细胞 |
| 子宫内膜细胞 | 肥大细胞 | 肿瘤细胞 |
| 内皮细胞 | 黑色素瘤细胞 |
使用方法
关于细胞解离的一般注意事项
一般来说，所需的胶原酶NB浓度取决于组织类型和来源以及分离程序。应将建议浓度视为起点浓度，并应目视监测消化过程的进展，以确定最佳条件。
我们建议按活性（PZ单位）而不是重量使用胶原酶NB。Nordmark保证了批次间的高度一致性；但是，可能会出现小的变化。因此，活动中的剂量可确保细胞分离过程的一致结果。有关优化组织消化的更多信息，请联系我们的技术支持！
关于分离细胞用于移植入人体
组织消化酶是组织工程和人体移植的关键原材料。Nordmark Biochemicals提供GMP级胶原酶NB产品，该产品由德国制药公司Nordmark根据当前GMP指南制造。欲了解更多信息，请联系我们！
以上按照种属，组织器官，细胞类别划分细胞解离需求，均可提供对应胶原酶的型号规格和推荐使用浓度，方法，以及有关参考文献。
胶原酶和水解酶活性比较
Nordmark的产品组合包括几种胶原酶NB和中性蛋白酶NB产品，可根据其纯化水平（未纯化、纯化、高纯化）和应用领域（研究、临床）进行区分。
| 品名 | 胶原酶活性 | 蛋白水解副作用 |
| Collagenase NB 4 Standard Grade | + | ++ |
| Collagenase NB 5 Sterile Grade | ||
| Collagenase NB 6 GMP Grade | ||
| Collagenase NB 4G Proved Grade | ++ | +++ |
| Collagenase NB 7D Gentle Grade | ++ | + |
| Collagenase NB 8 Broad Range | +++ | + |
| Collagenase NB 1 Premium Grade | ++++ | - |
| Collagenase NB 1 GMP Grade | ||
| Collagenase AF-1 GMP Grade |
不同阶段使用等级
客户体验
| Title | Reference | Cell Type |
| Cathepsin B Activity Initiates Apoptosis via Digestive Protease Activation in Pancreatic Acinar Cells and Experimental Pancreatitis | Sendler, M. et al. J. Biol. Chem. 2016, 291, 14717-14731. | Acinar cells |
| Signaling pathways involved in LPS induced TNFalpha production in human adipocytes | Hoareau, L. et al. Journal Inflamm.2010, 7. | Adipocytes |
| Ceiling Culture-Derived Proliferative Adipocytes are A Possible Delivery Vehicle for Enzyme Replacement Therapy in Lecithin: Cholesterol Acyltransferase Deficiency | Kuroda, M. et al. Open Gene Ther. J. 2011, 4, 1-10. | Adipocytes |
| Autologous fat transfer with in-situ mediation AIM a novel and compliant method of adult mesenchymal stem cell therapy | Wu, A. Y. and Morrow, D. J. Translat. Med. 2013, 11. | Adipose stem cells (ASC) |
| Development of fully defined xeno-free culture system for the preparation and propagation of cell therapy-compliant human adipose stem cells | Patrikoski, M. et al. Stem Cell Res Ther. 2013, 4, 27. | Adipose stem cells (ASC) |
| Xenofree Enzymatic Products for the Isolation of Human Adipose-Derived Stromal/Stem Cells | Carvalho,P. et al. Tiss Eng Part C Methods 2013, 19. | Adipose stem cells (ASC) |
| Human Adipose-Derived Mesenchymal Stem Cells in Cell Therapy: Safety and Feasibility in Different "Hospital Exemption" Clinical Applications | Veriter, S. et al. Plos One 2015, 10, e0139566. | Adipose stem cells (ASC) |
| A Standardized Method of Isolating Adipose-Derived Stem Cells for Clinical Applications | Raposio, E. et al. Annals Plast. Surg. 2015, 00. | Adipose stem cells (ASC) |
| Use of Bioengineered Bone for Sinus Augmentation | Beaumont, C. et al. J Periodontol 2008, 79, 541-548. | Bone |
| A nonaggressive, highly efficient, enzymatic method for dissociation of human braintumors and braintissues to viable singlecells | Volovitz, I. et al. BMC Neurosci. 2016, 17, 1-10. | Brain Cells |
| Cerebral endothelial cells release TNF-alpha after stimulation with cell walls of Streptococcus pneumoniae and regulate inducible nitric oxide synthase and ICAM-1 expression via autocrine loops | Freyer, D. et al. J. Immunol. 1999, 163, 4308-4314. | Cerebral endothelial cells |
| Atoh8 acts as a regulator of chondrocyte proliferation and differentiation in endochondral bones | Schroeder, N. et al. Plos One 2019, 14, e0218230. | Chondrozytes |
| Severe hepatic injury in interleukin 18 (IL-18) transgenic mice: a key role for IL-18 in regulating hepatocyte apoptosis in vivo | Finotto,S. et al. Gut 2004, 53, 392-400. | Hepatocytes |
| Hepatocyte differentiation of mesenchymal stem cells from human adipose tissue in vitro promotes hepatic integration in vivo | Aurich, H. et al. Gut 2009, 58, 570-581. | Hepatocytes |
| Cold Storage of Rat Hepatocyte Suspensions for One Week in a Customized Cold Storage Solution – Preservation of Cell Attachment and Metabolism | Pless-Petig, G. et al. PlosOne 2012, 7, e40444. | Hepatocytes |
| Comparison of methods for isolating primary hepatocytes from mini pigs | Li, Y. et al. Xenotransplantation2016, 0, 1-7. | Hepatocytes |
| Good manufacturing practice and clinical-grade human embryonic stem cell lines | Unger, C.Human Mol. Gen. 2008, 17, R48-R53. | Human embryonic stem cells (hESC) |
| Teratoma formation by human embryonic stem cells: Evaluation of essential parameters for future safety studies | Hentze, H. et al. Stem Cell Res. 2009, 2, 198-210. | Human embryonic stem cells (hESC) |
| Intrahepatic Administration of Human Liver Stem Cells in Infants with Inherited Neonatal-Onset Hyperammonemia: A Phase I Study | Spada, M. et al. Stem Cell Rev Rep. 2020, 16, 186-197. | Human Liver Stem Cells (HLSC) |
| Cryopreservation of human vascular umbilical cord cells under good manufacturing practice conditions for future cell banks | Polchow, B. et al. J Transl Med. 2012, 10, 98. | Human umbilical vein endothelial cells (HUVEC) |
| Successful Single Donor Islet Allotransplantation in the Streptozotocin Diabetes Rat Model | Jahr, H. Cell Transplant. 2002, 11, 513-518. | Islets of Langerhans |
| Tyrosine hydroxylase activity in the endocrine pancreas: changes induced by short-term dietary manipulation | Borelli, M. I. et al. BMC Endocr Disord. 2003, 3. | Islets of Langerhans |
| Influence of Collagenase Loading on Long-Term Preservation of Pig Pancreas by the Two-Layer Method for Sub sequent Islet Isolation | Brandhorst, D. et al. Transplant. 2005, 79,38-43. | Islets of Langerhans |
| Pancreas storage in oxygenated perfluorodecalin does not restore post-transplant function of isolated pig islets pre-damaged by warm ischemia. | Brandhorst, D. et al. Xenotransplant. 2006, 13, 465-470. | Islets of Langerhans |
| Current status of pancreatic islet transplantation | Merani, S. and Shaprio, A. M. J. Clin Sci (Lond.) 2006, 110, 611-625. | Islets of Langerhans |
| Quantification of Basal and Stimulated ROS Levels as Predictors of Islet Potency and Function | Armann, B et al. Am J Transplant. 2007, 7,38-47. | Islets of Langerhans |
| Factors Influencing the Collagenase Digestion Phase of Human Islet Isolation | Kin, T. et al. Transplant.2007, 83, 7-12. | Islets of Langerhans |
| Improvement of pancreatic islet cell isolation for transplantation | Matsumoto,S. et al. Proc (Bayl Univ Med Cent.) 2007, 20, 357-562. | Islets of Langerhans |
| Collagenase penetrates human pancreatic islets following standard intraductal administration | Cross, S.E. Transplant. 2008, 86, 907-911. | Islets of Langerhans |
| Iodixanol-Controlled Density Gradient During Islet Purification Improves Recovery Rate in Human Islet Isolation | Noguchi, H. et al. Transplant. 2009, 87, 1629-1635. | Islets of Langerhans |
| Pig Pancreas Oxygenation at 20°C Increases Islet ATP Generation but Deteriorates Islet Function | Iken, m. et al. Cell Transplant. 2009, 18, 745-751. | Islets of Langerhans |
| The Importance of Tryptic-like Activity in Purified Enzyme Blends for Efficient Islet Isolation | Brandhorst, H. et al. Transplant. 2009, 87, 370-375. | Islets of Langerhans |
| Human Islet Isolation for Autologous Transplantation: Comparison of Yield and Function Using SERVA/Nordmark Versus Roche Enzymes | Anazawa,T. et al. Am J Transplant. 2009, 9, 2383-2391. | Islets of Langerhans |
| Human Pancreatic Islet Isolation: Part I: Digestion and Collection of Pancreatic Tissue | Qi, M. J Vis Exp. 2009, 26, 1125-1126. | Islets of Langerhans |
| Low-Temperature Preservation of Isolated Islets is Superior to Conventional Islet Culture Before Islet Transplantation | Noguchi, H. et al. Transplant. 2010, 89, 47-54. | Islets of Langerhans |
| Experience of islet isolation without neutral protease supplementation | Kin, T. et al. Islets, 2010, 2, 278-282. | Islets of Langerhans |
| Islet Isolation for Clinical Transplantation | Kin, T. Adv Exp Med Biol. 2010, 654, 683-710. | Islets of Langerhans |
| A Novel Device for Islte Transplantation Providing Immune Protection and Oxygen Supply | Ludwig, B. et al.Horm Metab Res. 2010, 42, 918-922. | Islets of Langerhans |
| Agonist of growth hormone-releasing hormone as a potential effector for survival and proliferation of pancreatic islets | Ludwig, B. et al. PNAS 2010, 107, 12623-12628. | Islets of Langerhans |
| Assessment of human islet isolation with four different collagenases | Shimoda,M. et al. Transplant Proc. 2010, 42, 2049-2051. | Islets of Langerhans |
| Seven consecutive successful clinical islet isolations with pancreatic ductal injection | Matsumoto, S. et al. Cell Transplant. 2010, 19, 291-297. | Islets of Langerhans |
| Large-scale Comparison of Libearse HI and Collagenase NB 1 utilized for Himan Islet Isolation | Brandhorst,H. et al. Cell Transplant. 2010, 19, 3-8. | Islets of Langerhans |
| A Simplified Approach to Human Islet Quality Assessment | Hanson, M. S. et al. Transplant. 2010, 89, 1178-1188. | Islets of Langerhans |
| Evolution of β-Cell Replacement Therapy in DiabetesMellitus: Islet Cell Transplantation | Jahansouz, C. et al. Diabetes Technol Ther. 2011, 13, 395-418. | Islets of Langerhans |
| Recent progress in pancreatic islet transplantation | Kuise, t. and Noguchi, H. World J Transplant. 2011, 1, 13-18. | Islets of Langerhans |
| Impact of the number of infusions on 2-year results of islet-after-kidney transplantation in the GRAGIL network | Borot,S et al. Transplant. 2011, 92, 1031-1038. | Islets of Langerhans |
| Improved Pancreatic Islet Isolation Outcome in Autologous Transplantation for Chronic Pancreatitis | Naziruddin, B. et al Cell Transplant 2012, 21, 553-558. | Islets of Langerhans |
| Comparative impact on islet isolation and transplant outcome of the preservation solutions Institut Georges Lopez-1, University of Wisconsin, and Celsior | Niclauss, N. et al. Transplant. 2012, 93, 703-708. | Islets of Langerhans |
| Fresh Islets Are More Effective for Islet Transplantation Than Cultured Islets | Noguchi, H. et al. Cell Transplant. 2012, 21, 517-523. | Islets of Langerhans |
| Evaluation of Osmolality of Density Gradient for Human Islet Purification | Noguchi, H. et al. Cell Transplant. 2012, 21, 493-500. | Islets of Langerhans |
| Impact of Tissue Volume and Purification on Clinical Autologous Islet Transplantation for the Treatment of Chronic Pancreatitis | Matsumoto,S. et al. Cell Transplant. 2012, 21, 625-632. | Islets of Langerhans |
| Update on Islet Transplantation | McCall, M. and Shapiro, A. M. J. Cold Spring Harb Perspect Med. 2012, 2, a007823. | Islets of Langerhans |
| Improvement of collagenase distribution with the ductal preservation for human islet isolation | Shimoda, M. et al. Islets 2012, 4, 130-137. | Islets of Langerhans |
| Isolation of human islets for autologous islet transplantation in children and adolescents with chronic pancreatitis | Bottino,R et al. J Transplant. 2012, 642787. | Islets of Langerhans |
| Collagenase Does Not Persist in Human Islets Following Isolation | Cross, S. E. Cell Transplant. 2012, 21, 2531-2535. | Islets of Langerhans |
| Transplanted Functional Islet Mass: Donor, Islet Preparation, and Recipient Factors Influence Early Graft Function in Islet-After-Kidney Patients | Friberg, A. S. et al. Transplant. 2012, 93, 632-638. | Islets of Langerhans |
| Robot-assisted pancreatoduodenectomy with preservation of the vascular supply for autologous islet cell isolation and transplantation: a case report | Giulianotti, P. et al J Med case rep. 2012, 6. | Islets of Langerhans |
| Quality of Isolated Pig Islets Is Improved Using Perfluorohexyloctane for Pancreas Storage in a Split Lobe Model | Brandhorst, H. et al. Cell Transplant. 2013, 22, 1477-1483. | Islets of Langerhans |
| Clinical islet isolation outcomes with a highly purified neutral protease for pancreas dissociation | O'Gorman, D. et al. Islets, 2013, 5, 111-115. | Islets of Langerhans |
| Transplantation of Encapsulated Pancreatic Islets as a Treatment for Patients with Type 1 Diabetes Mellitus | Qi, M. Adv Med. 2014, 429710. | Islets of Langerhans |
| Human islet cell isolation: the initial step in an islet transplanting program in Shiraz, Southern Iran | Azarpira,N. et al. Exp Clin Transplant.2014, 12, 139-142. | Islets of Langerhans |
| Effects of Donor-, Pancreas-, and Isolation-Related Variables on Human Islet Isolation Outcome: A Systematic Review | Hilling, D. et al. Cell Transplant. 2014, 23, 921-928. | Islets of Langerhans |
| Islet Product Characteristics and Factors Related to Successful Human Islet Transplantation From the Collaborative Islet Transplant Registry (CITR) 1999–2010 | Balamurugan, A.N. et al. Am J Transplant 2014, 14, 2595-2606. | Islets of Langerhans |
| Autologous Islet Transplantation With Remote Islet Isolation After Pancreas Resection for Chronic Pancreatitis | Tai, D.S. et al. JAMA Surg. 2015, 150, 118-124. | Islets of Langerhans |
| National Institutes of Health–Sponsored Clinical Islet Transplantation Consortium Phase 3 Trial: Manufacture of a Complex Cellular Product at Eight Processing Facilities | Ricordi, C. et al. Diabetes 2016, 65, 3418-3428. | Islets of Langerhans |
| Enzyme Development for Human Islet Isolation: Five Decades of Progress or Stagnation? | Brandhorst, D. et al. Rev Diabet Stud, 2016, 14, 22-38. | Islets of Langerhans |
| Quantifying the Effects of Different Neutral Proteases on Human Islet Integrity | Brandhorst, H. et al. Cell Transplant 2017, 26, 1733-1741. | Islets of Langerhans |
| Evaluation of collagenase gold plus BP protease in isolating islets from human pancreata | Khiatah, B. Islets 2018, 4, 51-59. | Islets of Langerhans |
| Low Cost, Enriched Collagenase-Purified Protease Enzyme Mixtures Successfully Used for Human Islet Isolation | Loganathan, G. et al. OBM Transplantation 2019, 3. | Islets of Langerhans |
| Hypoxia-Preconditioned Wharton’s Jelly-Derived Mesenchymal Stem Cells Mitigate Stress-Induced Apoptosis and Ameliorate Human Islet Survival and Function in Direct Contact Coculture System |
Keshtkar, S. et al. StemCell Int. 2020. | Islets of Langerhans |
| Comparison of islet isolation result and clinical applicability according to GMP-grade collagenase enzyme blend in adult porcine islet isolation and culture | Kwak, K. et al. Xenotransplant. 2021, e12703. | Islets of Langerhans |
| Hepatocyte differentiation of mesenchymal stem cells from human adipose tissue in vitro promotes hepatic integration in vivo | Aurich, H. et al. Gut 2009, 58, 570-581. | Mesenchymal Stem Cells |
| Nonexpanded mesenchymal stem cells for regenerative medicine - yield in stromal vascular fraction from adipose-tissue | Faustini, M. et al. Tissue Engin. 2010, 16, 1515-1521. | Mesenchymal Stem Cells |
| A new standardized clinical-grade protocol for banking human umbilical cord tissue cells | Fazzina, R. et al. Transfusion 2015, 00, 1-10. | Mesenchymal Stem Cells |
| Efficient animal-serum free 3D cultivation method for adult human neural crest-derived stem cell therapeutics | Greiner, J. et al. Eur. Cells Mat. 2011, 22, 403-419. | Neural crest-derived stem cells |
| Culture bag systems for clinical applications of adult human neural crest-derived stem cells | Greiner, J. et al. Stem Cell Res Ther. 2014, 5, 34. | Neural crest-derived stem cells |
| Efficient Schwann cell purification by differential cell detachment using multiplex collagenase treatment | Jin,Y-Q. et al. J Neurosci Methods 2008, 170, 140-148. | Schwann Cells |
| The use of glandular-derived stem cells to improve vascularization in scaffold-mediated dermal regeneration | Egana, J. T. et al. Biomterials 2009, 30, 5918-5926. | Stem cells |
| Glandular Stem Cells: A New Source for Myocardial Repair? | Guldner, N. W. et al. Chapter in Reg. Medicine and Tiss. Eng. - Cells and Biomat. 2011 | Stem cells |
| Cellular modulation of polymeric device surfaces - promise of adult stem cells for neuro-prosthetics | Richter, A. et al. Front Neurosci. 2011, 5, 1-10. | Stem cells |
| Validation of an Automated Procedure to Isolate Human Adipose Tissue–Derived Cells by Using the Sepax Technology | Güven, S. et al. Tissue Engin. 2012, 18, 575-582. | Stromal Vascular Fraction |
| Development and Validation of a Fully GMP-Compliant Process for Manufacturing Stromal Vascular Fraction - A Cost-Effective Alternative to Automated Methods | Francois, P.Cells 2020, 9, 2158-2182. | Stromal Vascular Fraction |
| Pluripotency of adult stem cells derived from human and rat pancreas | Kruse,C. et al. Appl Phys.A 2004 | Pancreatic stellate-like cells (PSLCs) |
| Phenotypic heterogeneity among tumorigenic melanoma cells from patients that is reversible and not hierarchically organized | Quintana, E. et al. Cancer Cell 2010, 5, 510-523. | Tumor cells |
| A nonaggressive, highly efficient, enzymatic method for dissociation of human braintumors and braintissues to viable singlecells | Volovitz, I. et al. BMC Neurosci. 2016, 17,1-10. | Tumor cells |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
用户评价
暂无用户评价
文献和实验1-Cathepsin B Activity Initiates Apoptosis via Digestive Protease Activation in Pancreatic Acinar Cells and Experimental Pancreatitis
2-Signaling pathways involved in LPS induced TNFalpha production in human adipocytes
3-Ceiling Culture-Derived Proliferative Adipocytes are A Possible Delivery Vehicle for Enzyme Replacement Therapy in Lecithin
4-Autologous fat transfer with in-situ mediation AIM a novel and compliant method of adult mesenchymal stem cell therapy
5-Development of fully defined xeno-free culture system for the preparation and propagation of cell therapy-compliant human adipose stem cells
6-gmp_level_adipose_stem_cells_combined_with.3
7-Xenofree Enzymatic Products for the Isolation of Human Adipose-Derived Stromal
8-Human Adipose-Derived Mesenchymal Stem Cells in Cell Therapy
9-GMP Compliant Production of a Cryopreserved Adipose-Derived
10-Robot-assisted pancreatoduodenectomy with preservation of the vascular supply for autologous islet cell isolati
11-Atoh8 acts as a regulator of chondrocyte proliferation and differentiation in endochondral bones
12-Severe hepatic injury in interleukin 18 transgenic mice
13-Hepatocyte differentiation of mesenchymal stem cells from human adipose tissue in vitro promotes hepatic integration in vivo
14-Cold Storage of Rat Hepatocyte Suspensions for One Week in a Customized Cold Storage Solution – Preservation of Cell Attachment and Metabolism
15-Comparison of methods for isolating primary hepatocytes from mini pigs
16-Good manufacturing practice and clinical-grade human embryonic stem cell lines
17-Teratoma formation by human embryonic stem cells
18-Intrahepatic Administration of Human Liver Stem Cells in Infants with Inherited Neonatal-Onset Hyperammonemia
19-Cryopreservation of human vascular umbilical cord cells under good manufacturing practice conditions for future cell banks
20-Successful Single Donor Islet Allotransplantation in the Streptozotocin Diabetes Rat Model
21-Tyrosine hydroxylase activity in the endocrine pancreas changes induced by short-term dietary manipulation
22-Influence of Collagenase Loading on Long-Term Preservation of Pig Pancreas by the Two-Layer Method for Subsequent Islet Isolation
23-Pancreas storage in oxygenated perfluorodecalin does not restore post-transplant function of isolated pig islets pre-damaged by warm ischemia.
24-Current status of pancreatic islet transplantation
25-Quantification of Basal and Stimulated ROS Levels as Predictors of Islet Potency and Function
取出所需分装管，并在规定时间内使用完毕，避免整管长时间暴露于室温。 4. 机械解离法 vs 酶解法 vs 组合法？ 解离方法各有优缺点，根据不同组织类型和需求选择合适的解离方式。 （1）机械解离法：通过剪切、研磨、吹打等物理力破坏细胞间连接，无需酶试剂无需孵育时间成本较低，但是容易造成细胞损伤（如碎片多活率低）和解离不完全（如致密组织），常用于快速解离脾脏等柔软组织。 （2）酶解法：利用蛋白酶（胶原酶、胰酶、木瓜蛋白酶等）降解细胞外基质，温和解离保护细胞膜完整性，但是易破坏表位，且需要挑选合适
之一，其制备方案需根据脑区、发育阶段和疾病模型进行精细化调整和优化。如对于胚胎或者新生脑组织，其髓鞘化低、ECM 松散以透明质酸为主，需要注重解离过程的温和处理。而成年神经组织因髓鞘致密，ECM 交联显著。必须采用更高强度、多酶联合且配合机械剪切和密度梯度去髓鞘的策略，以兼顾解离效率与细胞存活。 针对不同肿瘤类型，单细胞悬液制备需「因瘤施策」：血液瘤离心即可。实体瘤则先机械剪切，再以胶原酶、透明质酸酶和 DNA 酶破除致密细胞外基质，其中富含纤维和 ECM 胶原的胰腺癌或乳腺癌需更长酶解。对于常规
酶法组织消化中常用的胶原酶（Collagenase），能特异性地降解胞外胶原蛋白，使胶原蛋白和纤维粘连蛋白的网状结构解离，而不损坏细胞表面结构的完整性。由于细胞外基质的成分复杂，除胶原蛋白外，还含有弹性蛋白，糖蛋白等其他大分子结构。而且不同组织类型，不同物种来源，不同年龄的组织中胞外基质组份不同。所以商业化的胶原酶产品一般是梭状芽孢杆菌裂解液的粗提物，是胶原酶、胰蛋白酶等蛋白水解酶混合物，能降解胶原蛋白和其他蛋白，及多糖等胞外大分子物质，适用于多种组织的消化应用。 但是对于不同实验
技术资料需要更多技术资料 索取更多技术资料
