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COL G，I型重组胶原酶

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  • ￥1817 - 11753
  • Abiel Biotech已认证
  • 意大利
  • 001-001/002/003
  • 2025年11月23日
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    青木生物技术（武汉）有限公司
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    细胞/组织解离新选择——重组胶原酶，批间一致性更佳，稳定性更好

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    • 保存条件：

      Lyophilized COL G and COL H are stable at -80℃ up to two years. We recommend splitting in aliquots the reconstituted solutions at need and storing them at -20℃ up to one month or -80℃ up to 6 months

    • 保质期：

      Lyophilized COL G and COL H are stable at -80℃ up to two years. We recommend splitting in aliquots the reconstituted solutions at need and storing them at -20℃ up to one month or -80℃ up to 6 months

    • 英文名：

      Recombinant Collagenases Class I (COL G)

    • 库存：

      请询

    • 供应商：

      青木生物技术（武汉）有限公司

    • 规格：

      75 U/300 U/750U

    规格：75 U产品价格：￥1817.0
    规格：300 U产品价格：￥5773.0
    规格：750U产品价格：￥11753.0
    1. DESCRIPTION
    COL G and COL H are recombinant collagenases (metalloproteinases) class I and class II respectively. COL G and COL H are synthesized separately from C. Histolyticum genes by DNA recombination in E. Coli BL21 AI strain, bearing a Maltose Binding Protein (MBP) tag at the N-terminal end. COL G and COL H are affinity chromatography purified proteins, highly pure, highly stable, lot-tolot consistent, endotoxin-free (≤ 10 EU/mg, LAL assay) and animal-free.
     
    2. APPLICATIONS
    Due to their high purity and specificity, COL G and COL H are especially indicated for the isolation of primary cells from liver, pancreas,
    heart, cartilage and stem cells from adipose tissue and others. In these applications we recommend using a combination of COL G and COL H in a specific activity ratio, or according to the relevant isolation protocol in order to obtain an optimal collagen digestion in cell isolation. F


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      图标文献和实验
      该产品被引用文献
      1. A poly-L-lactic acid/ collagen/glycosaminoglycan matrix for tissue engineering applications
      2. Isolation of Adult Rat Cardiomyocytes Using Recombinant Collagenases
      3. Biochemical Comparison Between Clostridium Hystoliticum Collagenases G and H obtained by DNA Recombinant and Extractive Procedures
      4. Tissue Dissociation and Primary Cells Isolation Using Recombinant Collagenases Class I and II
      5. A novel enzyme blend for efficient tissue dissociation and primary cells isolation
      6. Characterization of Collagenolytic/Proteolytic Marine Enzymes
      7. Production of Injectable Marine Collagen-Based Hydrogel for the Maintenance of Differentiated Chondrocytes in Tissue Engineering Applications
      8. Formulation of Different Chitosan Hydrogels for Cartilage Tissue Repair
      9. Neural Crest-Derived Chondrocytes Isolation for Tissue Engineering in Regenerative Medicine
      10. Vibrio Proteases for Biomedical Applications: Modulating the Proteolytic Secretome of V. alginolyticus and V. parahaemolyticus for Improved Enzymes Production
      11. APPLICATION OF RECOMBINANT COLLAGENASES ABIEL FOR FIBROBLAST EXTRACTION FROM HUMAN DERMA
      12. Hepatic NAPE-PLD Is a Key Regulator of Liver Lipid Metabolism
      13. 3D Collagen matrix improves islets of Langerhans differentiation and function
      14. Novel inulin-based mucoadhesive micelles loaded with corticosteroids as potential transcorneal permeation enhancers
      15. Mesenchymal stem cells derived from inflamed dental pulpal and gingival tissue: a potential application for bone formation
      16. Expansion of alpha beta T-cell receptor-bearing intestinal intraepithelial lymphocytes after microbial colonization in germ-free mice and its independence from thymus.
      17. Differential regulation of the NLRP 3 inflammasome and Caspase-1 by cigarette smoke extract in human macrophages
      18. Production of recombinant collagenases colg and colh in escherichia coli
      19. In Vitro and in Vivo investigation on stem cells isolated from pulp and gingival tissues from periodontally compromised teeth
      20. Comprehensive Analysis of a Vibrio parahaemolyticus Strain Extracellular Serine Protease VpSP37
      21. Optimization of a Biotechnological Process for Production and Purification of Two Recombinant Proteins: Col G and Col H
      22. "A New Method to Value Efficiency of Enzyme Blends for Pancreatic Tissue Digestion"
      23. Clostridium histolyticum recombinant collagenases and method for the manufacture thereof
      相关实验

      • 电穿孔转化 E.col

        transformation. Preparation of Electrocompetent Cells Materials •LB Broth Base [Life Technologies, cat # 12795-027]: add 25.0 g per liter of dH2O; autoclave at 121 ℃ for at least 15 minutes. Store at 4 ℃. •10% Glycerol: autoclave

      • 兔I型胶原（COL-1）酶联免疫分析（ELISA）

        兔 I型胶原（COL-1） 酶联免疫 分析（ ELISA ） 试剂 盒使用说明书 本试剂仅供研究使用          目的：本试剂盒用于测定兔血清，血浆及相关液体样本中 I型胶原（COL-1） 的 含量。 实验原理 ：    本试剂盒应用双抗体夹心法测定 标本 中 兔 I型胶原（ COL-1 ） 水平。用纯化的 兔 I型胶原 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入 I型胶原（ COL-1 ） 再与HRP 标记的 I型

      • 人I型胶原（COL-I）酶联免疫分析（ELISA）

        人 I 型胶原（COL-I ） 酶联免疫分析（ ELISA ） 试剂盒使用说明书 本试剂仅供研究使用         目的：本试剂盒用于测定人血清，血浆及相关液体样本中 I 型胶原（COL-1 ） 的 含量。 实验原理：   本试剂盒应用双抗体夹心法测定标本中人 I 型胶原（ COL-1 ） 水平。用纯化的人 I 型胶原 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入 I 型胶原（ COL

      查看更多相关实验 >
      图标技术资料

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      资料下载：

      COL-G-COL-H-Data-Sheet-2019.pdf 附 (下载 1 次)

      研选
      COL G，I型重组胶原酶
      ￥1817 - 11753