Anti-PECAM-1 (CD31), clone C10

Detection of Anti-GOR Antibodies in HCV Infection

The interactions of the hepatitis C virus (HCV) with the immune system are numerous. As one of the many results, a considerable number of autoantibodies occur in serum. This chapter describes methods to detect anti-GOR antibodies (1 –

从小鼠中分离内皮细胞

离心管中，用1 ml PBS洗磁珠两次，加入100 μg anti-CD31抗体到100 μl PBS到免疫磁珠并4°C慢摇24小时。用磁铁收集anti-CD31包被的磁珠。当管在磁铁里时去除上清，4°C用PBS洗磁珠洗3次，一次10分钟，最终4°C过夜。用磁铁收集磁珠，去除上清。4°C，用500 μl PBS/0.1%BSA重悬，使到达一个2×107beads/ml 的终浓度。3. 用磁珠分离内皮细胞 （1）在离心细胞后（200×g，5 min），加入15%FBS+M199。37°C

Immunodetection of cyclin D1 and D2/D3 using flow cytometry

-4326) or cyclin D2/D3 (cat. no. 14711A, clone G107-22). Other cyclin antibodies may be appropriate as well. Cy5-conjugated anti-mouse IgG (Jackson ImmunoResearch and Caltag) 0.5% paraformaldehyde in PBS 80% EtOH in ddH20 (stored at -20°C) cyclin