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文献和实验Clone Genes From a Phage Library
, LB tet, LB Kan, LB amp; Top agar: NZY or TB plus 0.7 percent agar autoclave); Cells: XLBlue MR strain, which is tetracycline resistant (on an F prime element; 25 micrograms/ml tet), and the SolR strain, which is kanamycin resistant (use 25 micrograms
在伯内特(F. M. Burnet)关于免疫的无性繁殖系选择学说中,对自身构成物质有发生免疫反应可能的某淋巴系细胞群,称为禁忌细胞株。禁忌细胞株虽依靠对自身的免疫耐受性而制止活动;但可以假定那种抑制机理一失败,那些细胞就对自身发生免疫反应,而出现自身免疫病。
Detection of Anti-GOR Antibodies in HCV Infection
The interactions of the hepatitis C virus (HCV) with the immune system are numerous. As one of the many results, a considerable number of autoantibodies occur in serum. This chapter describes methods to detect anti-GOR antibodies (1 –
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