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文献和实验:NuPAGE® 凝胶可在250V下跑24分钟 标准运行步骤 为了获得有效的分离,传统Laemmli凝胶需要跑90分钟。相比之下,NuPAGE® 凝胶的标准步骤则为200V,35分钟。此外,如果使用加速步骤,仅需24分钟就能获得有效分离(250V)。 图2. 加快运行时间,而不牺牲性能。NuPAGE® 样品上样:(1) Mark12™ 未染色标准品,10 μL;(2) 大鼠肝裂解液,10 μg;(3) 溶菌酶,6 μg;(4) Bio
E.Z.N.A.® Mag-Bind® Viral DNARNA Spin Protocol
containing detergent. DNA/RNA was bound to the surface of Mag-Bind® magnetic particles under proper condition. Proteins and cellular debris are efficiently washed with few wash steps. Pure RNA and DNA is then eluted in nuclease-free water or low ionic
E.Z.N.A.® Mag-Bind® Viral DNA/RNA Spin Protocol
containing detergent. DNA/RNA was bound to the surface of Mag-Bind® magnetic particles under proper condition. Proteins and cellular debris are efficiently washed with few wash steps. Pure RNA and DNA is then eluted in nuclease-free water or low ionic
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