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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
常温
- 库存:
现货
- 供应商:
联硕生物
- CAS号:
796963-92-3
- 规格:
1瓶
一般描述
磷脂酰甘油(PG)是在大肠埃希菌内膜中发现的膜脂质。[1]
应用
大肠埃希菌PG或L-α-磷脂酰甘油(大肠埃希菌)(钠盐)可用于:
作为脂质抗原以触发人类T细胞反应[2]
作为质谱法(MS)中与磷脂酰甘油(PG)结合的哺乳动物细胞进入(MCE)蛋白的标准品[3]
用于细菌模型膜中,以评估脂质-脂质各种相互作用,并有可能作为新型验证的模型[
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文献和实验A molecular basis of human T cell receptor autoreactivity toward self-phospholipids.
Adam Shahine et al.
Science immunology, 2(16) (2017-10-22)
Transformation of E. coli by Electroporation
Materials: SOB medium E. coli host strain such as DH5α WB tRNA 5 M ammonium acetate
In recent years several techniques have been described for the introduction of DNA molecules into Escherichia coli . These are based on the findings of Mandel and Higa (1 ), who demonstrated that incubation of cells with naked bacteriophage DNA
1.O/N (5ml E.coli in LB-->1ml into 200ml LB/37℃ 2.Grow 2-3H to A660 ~0.3-0.4 3.Chill cells 10'/ice Spin down 10',5K,4℃ 4.Wash 1X 200ml 10% glycerol (sterile)40℃; Spin down 10',5K,4℃ 5.Wash 1X 40 ml 10% glycerol--> transfer to S34 tubes & spin
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