Recombinant Human APRIL (N-Fla

Recombinant Human APRIL (N-Flag-His)(CU89)

产品说明(Description)

Recombinant Human A proliferation-inducing ligand is produced by our Mammalian expression system and the target gene encoding Lys112-Leu250 is expressed with a His, Flag tag at the N-terminus. 

Accession #: O75888

Known as: Tumor necrosis factor ligand superfamily member 13; A proliferation-inducing ligand; APRIL; TNF- and APOL-related leukocyte expressed ligand 2; TALL-2; TNF-related death ligand 1; TRDL-1; CD256; TNFSF13

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

 

质量控制(Quality Control) 

 

Purity:            Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/μg) as determined by LAL test.

 

Bioactivity-BLI:
Loaded Cynomolgus BCMA-Fc(Cat#CP59) on Protein A Biosensor, can bind  Human APRIL-His(Cat#CU89) with an affinity constant of 0.005 nM as determined in BLI assay.

 

复溶(Reconstitution)

Dissolve the lyophilized protein in distilled water.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily. It is synthesized as a 32 kDa proprotein which is cleaved by furin in the Golgi to release the active 17 kDa soluble molecule. Secreted human APRIL, which consists almost entirely of a single TNF homology domain, shares 85% amino acid sequence identity with mouse and rat APRIL. Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R. APRIL binds to heparan sulfate proteoglycans (HSPGs) independently of its binding to TACI and BCMA. APRIL can form bioactive heterotrimers with BAFF, and these circulate in the serum of patients with rheumatic immune disorders. APRIL enhances the proliferation and survival of plasma cells and also promotes T cell-dependent humoral responses. APRIL levels are elevated in the serum during coronary artery disease, and it is also elevated in many cancers primarily due to expression by tumor-infiltrating neutrophils.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY