Recombinant Human C5a

Recombinant Human C5a(CR52)
 

 

产品说明(Description)

Recombinant Human C5a is produced by our E.coli expression system and the target gene encoding Thr678-Arg751 is expressed. 

Accession #: P01031

Known as: Complement C5; C5a anaphylatoxin; C5a 
 

 

制剂（Formulation）

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
 

 

质量控制（Quality Control） 

 

Purity:            Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

   

 

复溶（Reconstitution）

Dissolve the lyophilized protein in distilled water.    

 

保存（Storage）

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.    

 

背景（Background）

Human Complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 74 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 α-chain. Human C5a has four α-helices plus three intrachain disulfide bonds that create a triple loop structure. Human C5a is 60% and 54% aa identical to mouse and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (C5aR/CD88) and a nonsignaling GPCR termed C5L2. Activation of Cd88 results in neutrophil chemotaxis and endothelial cell activation. It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY