Recombinant Human HLA-A*0201 N

Recombinant Human HLA-A*0201 NY-ESO-1 complex Protein (C-10His) (C10A)

产品说明(Description)

Recombinant Human HLA-A*0201 NY-ESO-1 complex Protein is produced by our Mammalian expression system and the target gene encoding (SLLMWITQC)&Ile21-Met119&Gly25-Ile308 (Ala269Val) is expressed with a 10His tag at the C-terminus.

Accession #: P61769&P01892

Known as: MHC; MY-ESO-1; HLA-A*0201 NY-ESO-1 complex Protein

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 500mM NaCl, pH7.4.

 

质量控制(Quality Control)

Purity:        Greater than 90% as determined by reducing SDS-PAGE.    

Endotoxin: Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

 

复溶(Reconstitution)

Always centrifuge tubes before opening.  Do not mix by vortex or pipetting. 

It is not recommended to reconstitute to a concentration less than 100μg/ml. 

Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. 
Aliquots of reconstituted samples are stable at < -20°C for 3 months. 

 

背景(Background)

NY-ESO-1 is a well-known cancer-testis antigen (CTAs) with re-expression in numerous cancer types. Its ability to elicit spontaneous humoral and cellular immune responses, together with its restricted expression pattern, have rendered it a good candidate target for cancer immunotherapy. Optimally assembled peptide-HLA-B2M trimer translocates to the surface of antigen-presenting cells, where it interacts with TCR and CD8 coreceptor on the surface of T cells.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY