Recombinant Human Neuropilin-1

Recombinant Human Neuropilin-1 (C-mFc) (C11P)

产品说明(Description)

Recombinant Human Neuropilin-1 is produced by our Mammalian expression system and the target gene encoding Phe22-Lys644 is expressed with a mFc tag at the C-terminus.

Accession #: AAH07533.1

Known as: Neuropilin-1, CD304; NRP1; NRPNP1; VEGF165R; BDCA4

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

 

质量控制(Quality Control)

Purity:        Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin: Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

Bioactivity-ELISA: 

 

Immobilized Anti-Human NRP1 mAb(Cat#NC064) at 2μg/ml (100 μl/well) can bind Human Neuropilin-1-mFc(Cat#C11P). The ED50 of Human Neuropilin-1-mFc(Cat#C11P) is 35.16 ng/ml.

 

复溶(Reconstitution)

Always centrifuge tubes before opening.  Do not mix by vortex or pipetting. 

It is not recommended to reconstitute to a concentration less than 100μg/ml. 

Dissolve the lyophilized protein in distilled water.

Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. 

Reconstituted protein solution can be stored at 4-7°C for 2-7 days.  

Aliquots of reconstituted samples are stable at < -20°C for 3 months. 

 

背景(Background)

Neuropilin-1 (Npn-1, previously neuropilin; also CD304) is a 130 - 140 kDa type I transmembrane (TM) glycoprotein that regulates axon guidance and angiogenesis. Two homologues, Neuropilin-1 and Neuropilin-2, are identified. Neuropilin-1 binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis. The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells. NRP1 expression is regulated in EC by tumor necrosis factor-alpha, the transcription factors dHAND and Ets-1, and vascular injury. NRP1 upregulation is positively correlated with the progression of various tumors.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY