Recombinant Human TIGIT (C-mFc

Recombinant Human TIGIT (C-mFc) (CW15)

产品说明(Description)

Recombinant Human T Cell Immunoreceptor With Ig And ITIM Domains is produced by our Mammalian expression system and the target gene encoding Met22-Pro141 is expressed with a mFc tag at the C-terminus. 

Accession #: Q495A1

Known as: T-cell immunoreceptor with Ig and ITIM domains;;VSIG9; VSTM3;TIGIT;V-set and transmembrane domain-containing protein 3;V-set and immunoglobulin domain-containing protein 9

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

 

质量控制(Quality Control) 

 

Purity:         Greater than 90% as determined by reducing SDS-PAGE.    

Endotoxin:  Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Bioactivity-BLI:   

 

Loaded Human PVR-His(Cat#C622) on HIS1K Biosensor, can bind Human TIGIT-mFc(Cat#CW15) with an affinity constant of 7.87 nM as determined in BLI assay.

 

 

复溶(Reconstitution)

Dissolve the lyophilized protein in distilled water.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

T cell immunoreceptor with Ig and ITIM domains (TIGIT) is a member of the CD28 family within the Ig superfamily of proteins. TIGIT is expressed on NK cells and subsets of activated, memory and regulatory T cells, and particularly on follicular helper T cells within secondary lymphoid organs. It binds to CD155 and Nectin-2 that appear on dendritic cells (DC) and endothelium. Ligation of TIGIT on T cells down-regulates TCR-mediated activation and subsequent proliferation, while NK cell TIGIT ligation blocks NK cell cytotoxicity. Through CD155 and Nectin-2, which also interact with DNAM-1/CD226 and CD96/Tactile, TIGIT is part of an interacting network of Ig superfamily members that may augment or oppose each other. In particular, TIGIT binding to CD155 can antagonize the effects of DNAM1.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY