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文献和实验Fastfilter Plasmid Midi Kit Spin Protocol
to completely neutralize the solution. Complete neutralization of the solution is vital of obtaining good yields. 6. Add 2 ml Buffer GBT, cover and gently mix by inverting tube 3-4 times. 7. Prepare the HiBind Midi Column. Place a HiBind Midi Column
Fungal Midi DNA Kit Protocol for Fresh/Frozen Specimens
that may have formed) to a HiBind® DNA Midi-column placed in a 15 mL collection tube (supplied) . Centrifuge the column at 8,000 x g for 5 min to bind DNA. Discard the flow-through liquid and reuse the collection tube. 6. Place column to a same collection tube
Materials Qiagen RNEasy Midi Kit Dounce homogenizers (for tissues) Syringes and needles (for cultured cells) 70% EtOH Sterile 15 mL conical tubes Procedure 1) Before using the reagents
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