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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20
- 保质期：
2年
- 英文名：
pAAV2-8
- 库存：
100
- 供应商：
上海烜雅生物科技有限公司
- 规格：
干粉/液体
基本信息
名称：pAAV2-8腺相关包装质粒
别称: pAAV2-8
质粒属性
质粒简介
pAAV2-8质粒由上海烜雅生物科技有限公司提供
质粒图谱
质粒序列
LOCUS Exported 21837 bp ds-DNA circular SYN 04-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..21837
/organism="synthetic DNA construct"
/mol_type="other DNA"
repeat_region 21..123
/label=ITR
/note="inverted terminal repeat of human adenovirus
serotype 5"
misc_signal 211..361
/label=Ad5 Psi
/note="packaging signal for adenovirus serotype 5"
CDS 1346..3211
/codon_start=1
/label=Rep
/translation="MPGFYEIVIKVPSDLDGHLPGISDSFVNWVAEKEWELPPDSDMDL
NLIEQAPLTVAEKLQRDFLTEWRRVSKAPEALFFVQFEKGESYFHMHVLVETTGVKSMV
LGRFLSQIREKLIQRIYRGIEPTLPNWFAVTKTRNGAGGGNKVVDECYIPNYLLPKTQP
ELQWAWTNMEQYLSACLNLTERKRLVAQHLTHVSQTQEQNKENQNPNSDAPVIRSKTSA
RYMELVGWLVDKGITSEKQWIQEDQASYISFNAASNSRSQIKAALDNAGKIMSLTKTAP
DYLVGQQPVEDISSNRIYKILELNGYDPQYAASVFLGWATKKFGKRNTIWLFGPATTGK
TNIAEAIAHTVPFYGCVNWTNENFPFNDCVDKMVIWWEEGKMTAKVVESAKAILGGSKV
RVDQKCKSSAQIDPTPVIVTSNTNMCAVIDGNSTTFEHQQPLQDRMFKFELTRRLDHDF
GKVTKQEVKDFFRWAKDHVVEVEHEFYVKKGGAKKRPAPSDADISEPKRVRESVAQPST
SDAEASINYADRYQNKCSRHVGMNLMLFPCRQCERMNQNSNICFTHGQKDCLECFPVSE
SQPVSVVKKAYQKLCYIHHIMGKVPDACTACDLVNVDLDDCIFEQ"
CDS 3228..5135
/codon_start=1
/label=Cap
/translation="MAADGYLPDWLEDNLSEGIREWWALKPGAPKPKANQQKQDDGRGL
VLPGYKYLGPFNGLDKGEPVNAADAAALEHDKAYDQQLQAGDNPYLRYNHADAEFQERL
QEDTSFGGNLGRAVFQAKKRVLEPLGLVEEGAKTAPGKKRPVEPSPQRSPDSSTGIGKK
GQQPARKRLNFGQTGDSESVPDPQPLGEPPAAPSGVGPNTMAAGGGAPM-ADNNEGADGV
GSSSGNWHCDSTWLGDRVITTSTRTWALPTYNNHLYKQISNGTSGGATNDNTYFGYS-TP
WGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLSFKLFNIQVKEVTQNEGTKTIANNLT
STIQVFTDSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQAVGRSSFYCLE
YFPSQMLRTGNNFQFTYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTQTTGGTA
NTQTLGFSQGGPNTMANQAKNWLPGPCYRQQRVSTTTGQNNNSNFAWTAGTKYHLNGRN
SLANPGIAMATHKDDEERFFPSNGILILANKMLPETMRITAMSCSPA?KKSKPLTLWLQ
RNTVSWQITCSSKTRLLKLELSTARGPYPVWSGRTGTCTCRVPSGPRFLTRTATSTRLR
"
repeat_region 19739..19841
/label=ITR
/note="inverted terminal repeat of human adenovirus
serotype 5"
rep_origin complement(20032..20620)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(20772..21632)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPATMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(21633..21737)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ggatccgaat tcttaattaa catcatcaat aatatacctt attttggatt gaagccaata
61 tgataatgag ggggtggagt ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg
121 tagtagtgtg gcggaagtgt gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg
181 tggcaaaagt gacgtttttg gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg
241 gttttaggcg gatgttgtag taaatttggg cgtaaccgag taagatttgg ccattttcgc
301 gggaaaactg aataagagga agtgaaatct gaataatttt gtgttactca tagcgcgtaa
361 tatttgtcta gggccgcggg gactttgacc gtttacgtgg agactcgccc aggtgttttt
421 ctcaggtgtt ttccgcgttc cgggtcaaag ttggcgtttt attattatag tcagggggat
481 cctctagaac tagtggatcc gtccctcagg cctagaagta aaaaagggaa aaaagagtgt
541 gtttgtcaaa ataggagaca ggtggtggca accaaggact tataggggac cttacatcta
601 cagaccaaca gatgccccct taccatatac aggaagatat gacttaaatt gggataggtg
661 ggtcacaatc aacggctata aagtgttata cagatccctc ccctcccctt tcgtgaaaga
721 ctcgccagag ctagacctcc ttggtgtatg ctaactgaga agagaaagac gacatgaaac
781 aacaggtaca tgattatatt tatctaggaa caggaatgca cttttgggga aaggttttcc
841 ataccaagga aggggcagtg gctggactga tagaacatta ttctgcaaaa acttatggta
901 tgagttatta tgattagcct ttatttgccc aaccttgcgg ttcccagggt ttaaataagt
961 ttatggttac aaactgttct taaaacgagg atgtgagaca agtggtttcc tgacttggtt
1021 tggtaatcaa atgttctgat ctgagctctt agtgttctat tttcctatgt tcttttggaa
1081 tctatccaag tcttatgtaa atgcttatgt aaaccataat ataaaagagt gctgattttt
1141 tgagtaaact tgcaacagtc ctaacattct tctctcgtgt gtttgtgtct gttcgccatc
1201 ccgtctccgc tcgtcactta tccttcactt ttcagagggt ccccccgcag atcccggtca
1261 ccctcaggtc gggacctgca gaagacgccc gagtgagcac gcagggtctc cattttgaag
1321 cgggaggttt gaacgcgcag ccgccatgcc ggggttttac gagattgtga ttaaggtccc
1381 cagcgacctt gacgggcatc tgcccggcat ttctgacagc tttgtgaact gggtggccga
1441 gaaggaatgg gagttgccgc cagattctga catggatctg aatctgattg agcaggcacc
1501 cctgaccgtg gccgagaagc tgcagcgcga ctttctgacg gaatggcgcc gtgtgagtaa
1561 ggccccggag gcccttttct ttgtgcaatt tgagaaggga gagagctact tccacatgca
1621 cgtgctcgtg gaaaccaccg gggtgaaatc catggttttg ggacgtttcc tgagtcagat
1681 tcgcgaaaaa ctgattcaga gaatttaccg cgggatcgag ccgactttgc caaactggtt
1741 cgcggtcaca aagaccagaa atggcgccgg aggcgggaac aaggtggtgg atgagtgcta
1801 catccccaat tacttgctcc ccaaaaccca gcctgagctc cagtgggcgt ggactaatat
1861 ggaacagtat ttaagcgcct gtttgaatct cacggagcgt aaacggttgg tggcgcagca
1921 tctgacgcac gtgtcgcaga cgcaggagca gaacaaagag aatcagaatc ccaattctga
1981 tgcgccggtg atcagatcaa aaacttcagc caggtacatg gagctggtcg ggtggctcgt
2041 ggacaagggg attacctcgg agaagcagtg gatccaggag gaccaggcct catacatctc
2101 cttcaatgcg gcctccaact cgcggtccca aatcaaggct gccttggaca atgcgggaaa
2161 gattatgagc ctgactaaaa ccgcccccga ctacctggtg ggccagcagc ccgtggagga
2221 catttccagc aatcggattt ataaaatttt ggaactaaac gggtacgatc cccaatatgc
2281 ggcttccgtc tttctgggat gggccacgaa aaagttcggc aagaggaaca ccatctggct
2341 gtttgggcct gcaactaccg ggaagaccaa catcgcggag gccatagccc acactgtgcc
2401 cttctacggg tgcgtaaact ggaccaatga gaactttccc ttcaacgact gtgtcgacaa
2461 gatggtgatc tggtgggagg aggggaagat gaccgccaag gtcgtggagt cggccaaagc
2521 cattctcgga ggaagcaagg tgcgcgtgga ccagaaatgc aagtcctcgg cccagataga
2581 cccgactccc gtgatcgtca cctccaacac caacatgtgc gccgtgattg acgggaactc
2641 aacgaccttc gaacaccagc agccgttgca agaccggatg ttcaaatttg aactcacccg
2701 ccgtctggat catgactttg ggaaggtcac caagcaggaa gtcaaagact ttttccggtg
2761 ggcaaaggat cacgtggttg aggtggagca tgaattctac gtcaaaaagg gtggagccaa
2821 gaaaagaccc gcccccagtg acgcagatat aagtgagccc aaacgggtgc gcgagtcagt
2881 tgcgcagcca tcgacgtcag acgcggaagc ttcgatcaac tacgcagaca ggtaccaaaa
2941 caaatgttct cgtcacgtgg gcatgaatct gatgctgttt ccctgcagac aatgcgagag
3001 aatgaatcag aattcaaata tctgcttcac tcacggacag aaagactgtt tagagtgctt
3061 tcccgtgtca gaatctcaac ccgtttctgt cgtcaaaaag gcgtatcaga aactgtgcta
3121 cattcatcat atcatgggaa aggtgccaga cgcttgcact gcctgcgatc tggtcaatgt
3181 ggatttggat gactgcatct ttgaacaata aatgatttaa atcaggtatg gctgccgatg
3241 gttatcttcc agattggctc gaggacaacc tctctgaggg cattcgcgag tggtgggcgc
3301 tgaaacctgg agccccgaag cccaaagcca accagcaaaa gcaggacgac ggccggggtc
3361 tggtgcttcc tggctacaag tacctcggac ccttcaacgg actcgacaag ggggagcccg
3421 tcaacgcggc ggacgcagcg gccctcgagc acgacaaggc ctacgaccag cagctgcagg
3481 cgggtgacaa tccgtacctg cggtataacc acgccgacgc cgagtttcag gagcgtctgc
3541 aagaagatac gtcttttggg ggcaacctcg ggcgagcagt cttccaggcc aagaagcggg
3601 ttctcgaacc tctcggtctg gttgaggaag gcgctaagac ggctcctgga aagaagagac
3661 cggtagagcc atcaccccag cgttctccag actcctctac gggcatcggc aagaaaggcc
3721 aacagcccgc cagaaaaaga ctcaattttg gtcagactgg cgactcagag tcagttccag
3781 accctcaacc tctcggagaa cctccagcag cgccctctgg tgtgggacct aatacaatgg
3841 ctgcaggcgg tggcgcacca atggcagaca ataacgaagg cgccgacgga gtgggtagtt
3901 cctcgggaaa ttggcattgc gattccacat ggctgggcga cagagtcatc accaccagca
3961 cccgaacctg ggccctgccc acctacaaca accacctcta caagcaaatc tccaacggga
4021 catcgggagg agccaccaac gacaacacct acttcggcta cagcaccccc tgggggtatt
4081 ttgactttaa cagattccac tgccactttt caccacgtga ctggcagcga ctcatcaaca
4141 acaactgggg attccggccc aagagactca gcttcaagct cttcaacatc caggtcaagg
4201 aggtcacgca gaatgaaggc accaagacca tcgccaataa cctcaccagc accatccagg
4261 tgtttacgga ctcggagtac cagctgccgt acgttctcgg ctctgcccac cagggctgcc
4321 tgcctccgtt cccggcggac gtgttcatga ttccccagta cggctaccta acactcaaca
4381 acggtagtca ggccgtggga cgctcctcct tctactgcct ggaatacttt ccttcgcaga
4441 tgctgagaac cggcaacaac ttccagttta cttacacctt cgaggacgtg cctttccaca
4501 gcagctacgc ccacagccag agcttggacc ggctgatgaa tcctctgatt gaccagtacc
4561 tgtactactt gtctcggact caaacaacag gaggcacggc aaatacgcag actctgggct
4621 tcagccaagg tgggcctaat acaatggcca atcaggcaaa gaactggctg ccaggaccct
4681 gttaccgcca acaacgcgtc tcaacgacaa ccgggcaaaa caacaatagc aactttgcct
4741 ggactgctgg gaccaaatac catctgaatg gaagaaattc attggctaat cctggcatcg
4801 ctatggcaac acacaaagac gacgaggagc gtttttttcc cagtaacggg atcctgattt
4861 tggcaaacaa aatgctgcca gagacaatgc ggattacagc gatgtcatgc tcaccagcga
4921 gnaagaaatc aaaaccacta accctgtggc tacagaggaa tacggtatcg tggcagataa
4981 cttgcagcag caaaacacgg ctcctcaaat tggaactgtc aacagccagg gggccttacc
5041 cggtatggtc tggcagaacc gggacgtgta cctgcagggt cccatctggg ccaagattcc
5101 tcacacggac ggcaacttcc acccgtctcc gctgatgggc ggctttggcc tgaaacatcc
5161 tccgcctcag atcctgatca agaacacgcc tgtacctgcg gatcctccga ccaccttcaa
5221 ccagtcaaag ctgaactctt tcatcacgca atacagcacc ggacaggtca gcgtggaaat
5281 tgaatgggag ctgcagaagg aaaacagcaa gcgctggaac cccgagatcc agtacacctc
5341 caactactac aaatctacaa gtgtggactt tgctgttaat acagaaggcg tgtactctga
5401 accccgcccc attggcaccc gttacctcac ccgtaatctg taattgcctg ttaatcaata
5461 aaccggttga ttcgtttcag ttgaactttg gtctctgcga agggcgaatt cgtttccatc
5521 gatgcatgtc cttgggtccg gcctgctgaa tgcgcaggcg gtcggccatg ccccaggctt
5581 cgttttgaca tcggcgcagg tctttgtagt agtcttgcat gagcctttct accggcactt
5641 cttcttctcc ttcctcttgt cctgcatctc ttgcatctat cgctgcggcg gcggcggagt
5701 ttggccgtag gtggcgccct cttcctccca tgcgtgtgac cccgaagccc ctcatcggct
5761 gaagcagggc taggtcggcg acaacgcgct cggctaatat ggcctgctgc acctgcgtga
5821 gggtagactg gaagtcatcc atgtccacaa agcggtggta tgcgcccgtg ttgatggtgt
5881 aagtgcagtt ggccataacg gaccagttaa cggtctggtg acccggctgc gagagctcgg
5941 tgtacctgag acgcgagtaa gccctcgagt caaatacgta gtcgttgcaa gtccgcacca
6001 ggtactggta tcccaccaaa aagtgcggcg gcggctggcg gtagaggggc cagcgtaggg
6061 tggccggggc tccgggggcg agatcttcca acataaggcg atgatatccg tagatgtacc
6121 tggacatcca ggtgatgccg gcggcggtgg tggaggcgcg cggaaagtcg cggacgcggt
6181 tccagatgtt gcgcagcggc aaaaagtgct ccatggtcgg gacgctctgg ccggtcaggc
6241 gcgcgcaatc gttgacgctc tagaccgtgc aaaaggagag cctgtaagcg ggcactcttc
6301 cgtggtctgg tggataaatt cgcaagggta tcatggcgga cgaccggggt tcgagccccg
6361 tatccggccg tccgccgtga tccatgcggt taccgcccgc gtgtcgaacc caggtgtgcg
6421 acgtcagaca acgggggagt gctccttttg gcttccttcc aggcgcggcg gctgctgcgc
6481 tagctttttt ggccactggc cgcgcgcagc gtaagcggtt aggctggaaa gcgaaagcat
6541 taagtggctc gctccctgta gccggagggt tattttccaa gggttgagtc gcgggacccc
6601 cggttcgagt ctcggaccgg ccggactgcg gcgaacgggg gtttgcctcc ccgtcatgca
6661 agaccccgct tgcaaattcc tccggaaaca gggacgagcc ccttttttgc ttttcccaga
6721 tgcatccggt gctgcggcag atgcgccccc ctcctcagca gcggcaagag caagagcagc
6781 ggcagacatg cagggcaccc tcccctcctc ctaccgcgtc aggaggggcg acatccgcgg
6841 ttgacgcggc agcagatggt gattacgaac ccccgcggcg ccgggcccgg cactacctgg
6901 acttggagga gggcgagggc ctggcgcggc taggagcgcc ctctcctgag cggtacccaa
6961 gggtgcagct gaagcgtgat acgcgtgagg cgtacgtgcc gcggcagaac ctgtttcgcg
7021 accgcgaggg agaggagccc gaggagatgc gggatcgaaa gttccacgca gggcgcgagc
7081 tgcggcatgg cctgaatcgc gagcggttgc tgcgcgagga ggactttgag cccgacgcgc
7141 gaaccgggat tagtcccgcg cgcgcacacg tggcggccgc cgacctggta accgcatacg
7201 agcagacggt gaaccagggc gatcgcaccc tttggcgcat cccattctcc agtaacttta
7261 tgtccatggg cgcactcaca gacctgggcc aaaaccttct ctacgccaac tccgcccacg
7321 cgctagacat gacttttgag gtggatccca tggacgagcc cacccttctt tatgttttgt
7381 ttgaagtctt tgacgtggtc cgtgtgcacc ggccgcaccg cggcgtcatc gaaaccgtgt
7441 acctgcgcac gcccttctcg gccggcaacg ccacaacata aagaagcaag caacatcaac
7501 aacagctgcc gccatgggct ccagtgagca ggaactgaaa gccattgtca aagatcttgg
7561 ttgtgggcca tattttttgg gcacctatga caagcgcttt ccaggctttg tttctccaca
7621 caagctcgcc tgcgccatag tcaatacggc cggtcgcgag actgggggcg tacactggat
7681 ggcctttgcc tggaacccgc actcaaaaac atgctacctc tttgagccct ttggcttttc
7741 tgaccagcga ctcaagcagg tttaccagtt tgagtacgag tcactcctgc gccgtagcgc
7801 cattgcttct tcccccgacc gctgtataac gctggaaaag tccacccaaa gcgtacaggg
7861 gcccaactcg gccgcctgtg gactattctg ctgcatgttt ctccacgcct ttgccaactg
7921 gccccaaact cccatggatc acaaccccac catgaacctt attaccgggg tacccaactc
7981 catgctcaac agtccccagg tacagcccac cctgcgtcgc aaccaggaac agctctacag
8041 cttcctggag cgccactcgc cctacttccg cagccacagt gcgcagatta ggagcgccac
8101 ttctttttgt cacttgaaaa acatgtaaaa ataatgtact agagacactt tcaataaagg
8161 caaatgcttt tatttgtaca ctctcgggtg attatttacc cccacccttg ccgtctgcgc
8221 cgtttaaaaa tcaaaggggt tctgccgcgc atcgctatgc gccactggca gggacacgtt
8281 gcgatactgg tgtttagtgc tccacttaaa ctcaggcaca accatccgcg gcagctcggt
8341 gaagttttca ctccacaggc tgcgcaccat caccaacgcg tttagcaggt cgggcgccga
8401 tatcttgaag tcgcagttgg ggcctccgcc ctgcgcgcgc gagttgcgat acacagggtt
8461 gcagcactgg aacactatca gcgccgggtg gtgcacgctg gccagcacgc tcttgtcgga
8521 gatcagatcc gcgtccaggt cctccgcgtt gctcagggcg aacggagtca actttggtag
8581 ctgccttccc aaaaagggcg cgtgcccagg ctttgagttg cactcgcacc gtagtggcat
8641 caaaaggtga ccgtgcccgg tctgggcgtt aggatacagc gcctgcataa aagccttgat
8701 ctgcttaaaa gccacctgag cctttgcgcc ttcagagaag aacatgccgc aagacttgcc
8761 ggaaaactga ttggccggac aggccgcgtc gtgcacgcag caccttgcgt cggtgttgga
8821 gatctgcacc acatttcggc cccaccggtt cttcacgatc ttggccttgc tagactgctc
8881 cttcagcgcg cgctgcccgt tttcgctcgt cacatccatt tcaatcacgt gctccttatt
8941 tatcataatg cttccgtgta gacacttaag ctcgccttcg atctcagcgc agcggtgcag
9001 ccacaacgcg cagcccgtgg gctcgtgatg cttgtaggtc acctctgcaa acgactgcag
9061 gtacgcctgc aggaatcgcc ccatcatcgt cacaaaggtc ttgttgctgg tgaaggtcag
9121 ctgcaacccg cggtgctcct cgttcagcca ggtcttgcat acggccgcca gagcttccac
9181 ttggtcaggc agtagtttga agttcgcctt tagatcgtta tccacgtggt acttgtccat
9241 cagcgcgcgc gcagcctcca tgcccttctc ccacgcagac acgatcggca cactcagcgg
9301 gttcatcacc gtaatttcac tttccgcttc gctgggctct tcctcttcct cttgcgtccg
9361 cataccacgc gccactgggt cgtcttcatt cagccgccgc actgtgcgct tacctccttt
9421 gccatgcttg attagcaccg gtgggttgct gaaacccacc atttgtagcg ccacatcttc
9481 tctttcttcc tcgctgtcca cgattacctc tggtgatggc gggcgctcgg gcttgggaga
9541 agggcgcttc tttttcttct tgggcgcaat ggccaaatcc gccgccgagg tcgatggccg
9601 cgggctgggt gtgcgcggca ccagcgcgtc ttgtgatgag tcttcctcgt cctcggactc
9661 gatacgccgc ctcatccgct tttttggggg cgcccgggga ggcggcggcg acggggacgg
9721 ggacgacacg tcctccatgg ttgggggacg tcgcgccgca ccgcgtccgc gctcgggggt
9781 ggtttcgcgc tgctcctctt cccgactggc catttccttc tcctataggc agaaaaagat
9841 catggagtca gtcgagaaga aggacagcct aaccgccccc tctgagttcg ccaccaccgc
9901 ctccaccgat gccgccaacg cgcctaccac cttccccgtc gaggcacccc cgcttgagga
9961 ggaggaagtg attatcgagc aggacccagg ttttgtaagc gaagacgacg aggaccgctc
10021 agtaccaaca gaggataaaa agcaagacca ggacaacgca gaggcaaacg aggaacaagt
10081 cgggcggggg gacgaaaggc atggcgacta cctagatgtg ggagacgacg tgctgttgaa
10141 gcatctgcag cgccagtgcg ccattatctg cgacgcgttg caagagcgca gcgatgtgcc
10201 cctcgccata gcggatgtca gccttgccta cgaacgccac ctattctcac cgcgcgtacc
10261 ccccaaacgc caagaaaacg gcacatgcga gcccaacccg cgcctcaact tctaccccgt
10321 atttgccgtg ccagaggtgc ttgccaccta tcacatcttt ttccaaaact gcaagatacc
10381 cctatcctgc cgtgccaacc gcagccgagc ggacaagcag ctggccttgc ggcagggcgc
10441 tgtcatacct gatatcgcct cgctcaacga agtgccaaaa atctttgagg gtcttggacg
10501 cgacgagaag cgcgcggcaa acgctctgca acaggaaaac agcgaaaatg aaagtcactc
10561 tggagtgttg gtggaactcg agggtgacaa cgcgcgccta gccgtactaa aacgcagcat
10621 cgaggtcacc cactttgcct acccggcact taacctaccc cccaaggtca tgagcacagt
10681 catgagtgag ctgatcgtgc gccgtgcgca gcccctggag agggatgcaa atttgcaaga
10741 acaaacagag gagggcctac ccgcagttgg cgacgagcag ctagcgcgct ggcttcaaac
10801 gcgcgagcct gccgacttgg aggagcgacg caaactaatg atggccgcag tgctcgttac
10861 cgtggagctt gagtgcatgc agcggttctt tgctgacccg gagatgcagc gcaagctaga
10921 ggaaacattg cactacacct ttcgacaggg ctacgtacgc caggcctgca agatctccaa
10981 cgtggagctc tgcaacctgg tctcctacct tggaattttg cacgaaaacc gccttgggca
11041 aaacgtgctt cattccacgc tcaagggcga ggcgcgccgc gactacgtcc gcgactgcgt
11101 ttacttattt ctatgctaca cctggcagac ggccatgggc gtttggcagc agtgcttgga
11161 ggagtgcaac ctcaaggagc tgcagaaact gctaaagcaa aacttgaagg acctatggac
11221 ggccttcaac gagcgctccg tggccgcgca cctggcggac atcattttcc ccgaacgcct
11281 gcttaaaacc ctgcaacagg gtctgccaga cttcaccagt caaagcatgt tgcagaactt
11341 taggaacttt atcctagagc gctcaggaat cttgcccgcc acctgctgtg cacttcctag
11401 cgactttgtg cccattaagt accgcgaatg ccctccgccg ctttggggcc actgctacct
11461 tctgcagcta gccaactacc ttgcctacca ctctgacata atggaagacg tgagcggtga
11521 cggtctactg gagtgtcact gtcgctgcaa cctatgcacc ccgcaccgct ccctggtttg
11581 caattcgcag ctgcttaacg aaagtcaaat tatcggtacc tttgagctgc agggtccctc
11641 gcctgacgaa aagtccgcgg ctccggggtt gaaactcact ccggggctgt ggacgtcggc
11701 ttaccttcgc aaatttgtac ctgaggacta ccacgcccac gagattaggt tctacgaaga
11761 ccaatcccgc ccgccaaatg cggagcttac cgcctgcgtc attacccagg gccacattct
11821 tggccaattg caagccatca acaaagcccg ccaagagttt ctgctacgaa agggacgggg
11881 ggtttacttg gacccccagt ccggcgagga gctcaaccca atccccccgc cgccgcagcc
11941 ctatcagcag cagccgcggg cccttgcttc ccaggatggc acccaaaaag aagctgcagc
12001 tgccgccgcc acccacggac gaggaggaat actgggacag tcaggcagag gaggttttgg
12061 acgaggagga ggaggacatg atggaagact gggagagcct agacgaggaa gcttccgagg
12121 tcgaagaggt gtcagacgaa acaccgtcac cctcggtcgc attcccctcg ccggcgcccc
12181 agaaatcggc aaccggttcc agcatggcta caacctccgc tcctcaggcg ccgccggcac
12241 tgcccgttcg ccgacccaac cgtagatggg acaccactgg aaccagggcc ggtaagtcca
12301 agcagccgcc gccgttagcc caagagcaac aacagcgcca aggctaccgc tcatggcgcg
12361 ggcacaagaa cgccatagtt gcttgcttgc aagactgtgg gggcaacatc tccttcgccc
12421 gccgctttct tctctaccat cacggcgtgg ccttcccccg taacatcctg cattactacc
12481 gtcatctcta cagcccatac tgcaccggcg gcagcggcag cggcagcaac agcagcggcc
12541 acacagaagc aaaggcgacc ggatagcaag actctgacaa agcccaagaa atccacagcg
12601 gcggcagcag caggaggagg agcgctgcgt ctggcgccca acgaacccgt atcgacccgc
12661 gagcttagaa acaggatttt tcccactctg tatgctatat ttcaacagag caggggccaa
12721 gaacaagagc tgaaaataaa aaacaggtct ctgcgatccc tcacccgcag ctgcctgtat
12781 cacaaaagcg aagatcagct tcggcgcacg ctggaagacg cggaggctct cttcagtaaa
12841 tactgcgcgc tgactcttaa ggactagttt cgcgcccttt ctcaaattta agcgcgaaaa
12901 ctacgtcatc tccagcggcc acacccggcg ccagcacctg tcgtcagcgc cattatgagc
12961 aaggaaattc ccacgcccta catgtggagt taccagccac aaatgggact tgcggctgga
13021 gctgcccaag actactcaac ccgaataaac tacatgagcg cgggacccca catgatatcc
13081 cgggtcaacg gaatccgcgc ccaccgaaac cgaattctct tggaacaggc ggctattacc
13141 accacacctc gtaataacct taatccccgt agttggcccg ctgccctggt gtaccaggaa
13201 agtcccgctc ccaccactgt ggtacttccc agagacgccc aggccgaagt tcagatgact
13261 aactcagggg cgcagcttgc gggcggcttt cgtcacaggg tgcggtcgcc cgggcagggt
13321 ataactcacc tgacaatcag agggcgaggt attcagctca acgacgagtc ggtgagctcc
13381 tcgcttggtc tccgtccgga cgggacattt cagatcggcg gcgccggccg ctcttcattc
13441 acgcctcgtc aggcaatcct aactctgcag acctcgtcct ctgagccgcg ctctggaggc
13501 attggaactc tgcaatttat tgaggagttt gtgccatcgg tctactttaa ccccttctcg
13561 ggacctcccg gccactatcc ggatcaattt attcctaact ttgacgcggt aaaggactcg
13621 gcggatggct acgactgaat gttaagtgga gaggcagagc aactgcgcct gaaacacctg
13681 gtccactgtc gccgccacaa gtgctttgcc cgcgactccg gtgagttttg ctactttgaa
13741 ttgcccgagg atcatatcga gggcccggcg cacggcgtcc ggcttaccgc ccagggagag
13801 cttgcccgta gcctgattcg ggagtttacc cagcgccccc tgctagttga gcgggacagg
13861 ggaccctgtg ttctcactgt gatttgcaac tgtcctaacc ctggattaca tcaagatctt
13921 tgttgccatc tctgtgctga gtataataaa tacagaaatt aaaatatact ggggctccta
13981 tcgccatcct gtaaacgcca ccgtcttcac ccgcccaagc aaaccaaggc gaaccttacc
14041 tggtactttt aacatctctc cctctgtgat ttacaacagt ttcaacccag acggagtgag
14101 tctacgagag aacctctccg agctcagcta ctccatcaga aaaaacacca ccctccttac
14161 ctgccgggaa cgtacgagtg cgtcaccggc cgctgcacca cacctaccgc ctgaccgtaa
14221 accagacttt ttccggacag acctcaataa ctctgtttac cagaacagga ggtgagctta
14281 gaaaaccctt agggtattag gccaaaggcg cagctactgt ggggtttatg aacaattcaa
14341 gcaactctac gggctattct aattcaggtt tctctagaaa tggacggaat tattacagag
14401 cagcgcctgc tagaaagacg cagggcagcg gccgagcaac agcgcatgaa tcaagagctc
14461 caagacatgg ttaacttgca ccagtgcaaa aggggtatct tttgtctggt aaagcaggcc
14521 aaagtcacct acgacagtaa taccaccgga caccgcctta gctacaagtt gccaaccaag
14581 cgtcagaaat tggtggtcat ggtgggagaa aagcccatta ccataactca gcactcggta
14641 gaaaccgaag gctgcattca ctcaccttgt caaggacctg aggatctctg cacccttatt
14701 aagaccctgt gcggtctcaa agatcttatt ccctttaact aataaaaaaa aataataaag
14761 catcacttac ttaaaatcag ttagcaaatt tctgtccagt ttattcagca gcacctcctt
14821 gccctcctcc cagctctggt attgcagctt cctcctggct gcaaactttc tccacaatct
14881 aaatggaatg tcagtttcct cctgttcctg tccatccgca cccactatct tcatgttgtt
14941 gcagatgaag cgcgcaagac cgtctgaaga taccttcaac cccgtgtatc catatgacac
15001 ggaaaccggt cctccaactg tgccttttct tactcctccc tttgtatccc ccaatgggtt
15061 tcaagagagt ccccctgggg tactctcttt gcgcctatcc gaacctctag ttacctccaa
15121 tggcatgctt gcgctcaaaa tgggcaacgg cctctctctg gacgaggccg gcaaccttac
15181 ctcccaaaat gtaaccactg tgagcccacc tctcaaaaaa accaagtcaa acataaacct
15241 ggaaatatct gcacccctca cagttacctc agaagcccta actgtggctg ccgccgcacc
15301 tctaatggtc gcgggcaaca cactcaccat gcaatcacag gccccgctaa ccgtgcacga
15361 ctccaaactt agcattgcca cccaaggacc cctcacagtg tcagaaggaa agctagccct
15421 gcaaacatca ggccccctca ccaccaccga tagcagtacc cttactatca ctgcctcacc
15481 ccctctaact actgccactg gtagcttggg cattgacttg aaagagccca tttatacaca
15541 aaatggaaaa ctaggactaa agtacggggc tcctttgcat gtaacagacg acctaaacac
15601 tttgaccgta gcaactggtc caggtgtgac tattaataat acttccttgc aaactaaagt
15661 tactggagcc ttgggttttg attcacaagg caatatgcaa cttaatgtag caggaggact
15721 aaggattgat tctcaaaaca gacgccttat acttgatgtt agttatccgt ttgatgctca
15781 aaaccaacta aatctaagac taggacaggg ccctcttttt ataaactcag cccacaactt
15841 ggatattaac tacaacaaag gcctttactt gtttacagct tcaaacaatt ccaaaaagct
15901 tgaggttaac ctaagcactg ccaaggggtt gatgtttgac gctacagcca tagccattaa
15961 tgcaggagat gggcttgaat ttggttcacc taatgcacca aacacaaatc ccctcaaaac
16021 aaaaattggc catggcctag aatttgattc aaacaaggct atggttccta aactaggaac
16081 tggccttagt tttgacagca caggtgccat tacagtagga aacaaaaata atgataagct
16141 aactttgtgg accacaccag ctccatctcc taactgtaga ctaaatgcag agaaagatgc
16201 taaactcact ttggtcttaa caaaatgtgg cagtcaaata cttgctacag tttcagtttt
16261 ggctgttaaa ggcagtttgg ctccaatatc tggaacagtt caaagtgctc atcttattat
16321 aagatttgac gaaaatggag tgctactaaa caattccttc ctggacccag aatattggaa
16381 ctttagaaat ggagatctta ctgaaggcac agcctataca aacgctgttg gatttatgcc
16441 taacctatca gcttatccaa aatctcacgg taaaactgcc aaaagtaaca ttgtcagtca
16501 agtttactta aacggagaca aaactaaacc tgtaacacta accattacac taaacggtac
16561 acaggaaaca ggagacacaa ctccaagtgc atactctatg tcattttcat gggactggtc
16621 tggccacaac tacattaatg aaatatttgc cacatcctct tacacttttt catacattgc
16681 ccaagaataa agaatcgttt gtgttatgtt tcaacgtgtt tatttttcaa ttgcagaaaa
16741 tttcaagtca tttttcattc agtagtatag ccccaccacc acatagctta tacagatcac
16801 cgtaccttaa tcaaactcac agaaccctag tattcaacct gccacctccc tcccaacaca
16861 cagagtacac agtcctttct ccccggctgg ccttaaaaag catcatatca tgggtaacag
16921 acatattctt aggtgttata ttccacacgg tttcctgtcg agccaaacgc tcatcagtga
16981 tattaataaa ctccccgggc agctcactta agttcatgtc gctgtccagc tgctgagcca
17041 caggctgctg tccaacttgc ggttgcttaa cgggcggcga aggagaagtc cacgcctaca
17101 tgggggtaga gtcataatcg tgcatcagga tagggcggtg gtgctgcagc agcgcgcgaa
17161 taaactgctg ccgccgccgc tccgtcctgc aggaatacaa catggcagtg gtctcctcag
17221 cgatgattcg caccgcccgc agcataaggc gccttgtcct ccgggcacag cagcgcaccc
17281 tgatctcact taaatcagca cagtaactgc agcacagcac cacaatattg ttcaaaatcc
17341 cacagtgcaa ggcgctgtat ccaaagctca tggcggggac cacagaaccc acgtggccat
17401 cataccacaa gcgcaggtag attaagtggc gacccctcat aaacacgctg gacataaaca
17461 ttacctcttt tggcatgttg taattcacca cctcccggta ccatataaac ctctgattaa
17521 acatggcgcc atccaccacc atcctaaacc agctggccaa aacctgcccg ccggctatac
17581 actgcaggga accgggactg gaacaatgac agtggagagc ccaggactcg taaccatgga
17641 tcatcatgct cgtcatgata tcaatgttgg cacaacacag gcacacgtgc atacacttcc
17701 tcaggattac aagctcctcc cgcgttagaa ccatatccca gggaacaacc cattcctgaa
17761 tcagcgtaaa tcccacactg cagggaagac ctcgcacgta actcacgttg tgcattgtca
17821 aagtgttaca ttcgggcagc agcggatgat cctccagtat ggtagcgcgg gtttctgtct
17881 caaaaggagg tagacgatcc ctactgtacg gagtgcgccg agacaaccga gatcgtgttg
17941 gtcgtagtgt catgccaaat ggaacgccgg acgtagtcat atttcctgaa gcaaaaccag
18001 gtgcgggcgt gacaaacaga tctgcgtctc cggtctcgcc gcttagatcg ctctgtgtag
18061 tagttgtagt atatccactc tctcaaagca tccaggcgcc ccctggcttc gggttctatg
18121 taaactcctt catgcgccgc tgccctgata acatccacca ccgcagaata agccacaccc
18181 agccaaccta cacattcgtt ctgcgagtca cacacgggag gagcgggaag agctggaaga
18241 accatgtttt tttttttatt ccaaaagatt atccaaaacc tcaaaatgaa gatctattaa
18301 gtgaacgcgc tcccctccgg tggcgtggtc aaactctaca gccaaagaac agataatggc
18361 atttgtaaga tgttgcacaa tggcttccaa aaggcaaacg gccctcacgt ccaagtggac
18421 gtaaaggcta aacccttcag ggtgaatctc ctctataaac attccagcac cttcaaccat
18481 gcccaaataa ttctcatctc gccaccttct caatatatct ctaagcaaat cccgaatatt
18541 aagtccggcc attgtaaaaa tctgctccag agcgccctcc accttcagcc tcaagcagcg
18601 aatcatgatt gcaaaaattc aggttcctca cagacctgta taagattcaa aagcggaaca
18661 ttaacaaaaa taccgcgatc ccgtaggtcc cttcgcaggg ccagctgaac ataatcgtgc
18721 aggtctgcac ggaccagcgc ggccacttcc ccgccaggaa ccatgacaaa agaacccaca
18781 ctgattatga cacgcatact cggagctatg ctaaccagcg tagccccgat gtaagcttgt
18841 tgcatgggcg gcgatataaa atgcaaggtg ctgctcaaaa aatcaggcaa agcctcgcgc
18901 aaaaaagaaa gcacatcgta gtcatgctca tgcagataaa ggcaggtaag ctccggaacc
18961 accacagaaa aagacaccat ttttctctca aacatgtctg cgggtttctg cataaacaca
19021 aaataaaata acaaaaaaac atttaaacat tagaagcctg tcttacaaca ggaaaaacaa
19081 cccttataag cataagacgg actacggcca tgccggcgtg accgtaaaaa aactggtcac
19141 cgtgattaaa aagcaccacc gacagctcct cggtcatgtc cggagtcata atgtaagact
19201 cggtaaacac atcaggttga ttcacatcgg tcagtgctaa aaagcgaccg aaatagcccg
19261 ggggaataca tacccgcagg cgtagagaca acattacagc ccccatagga ggtataacaa
19321 aattaatagg agagaaaaac acataaacac ctgaaaaacc ctcctgccta ggcaaaatag
19381 caccctcccg ctccagaaca acatacagcg cttccacagc ggcagccata acagtcagcc
19441 ttaccagtaa aaaagaaaac ctattaaaaa aacaccactc gacacggcac cagctcaatc
19501 agtcacagtg taaaaaaggg ccaagtgcag agcgagtata tataggacta aaaaatgacg
19561 taacggttaa agtccacaaa aaacacccag aaaaccgcac gcgaacctac gcccagaaac
19621 gaaagccaaa aaacccacaa cttcctcaaa tcgtcacttc cgttttccca cgttacgtca
19681 cttcccattt taagaaaact acaattccca acacatacaa gttactccgc cctaaaacct
19741 acgtcacccg ccccgttccc acgccccgcg ccacgtcaca aactccaccc cctcattatc
19801 atattggctt caatccaaaa taaggtatat tattgatgat gttaattaag aattcggatc
19861 tgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg
19921 gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct
19981 tcacggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
20041 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
20101 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
20161 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
20221 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
20281 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
20341 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
20401 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
20461 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
20521 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
20581 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
20641 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
20701 atcaaaaagg atcttcacct agatcctttt aaatcaatct aaagtatata tgagtaaact
20761 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
20821 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
20881 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
20941 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
21001 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
21061 agtttgcgca acgttgttgc cattgttgca ggcatcgtgg tgtcacgctc gtcgtttggt
21121 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
21181 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
21241 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
21301 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
21361 cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact
21421 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
21481 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
21541 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
21601 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
21661 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
21721 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
21781 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaa
//
质粒菌株产品操作说明书
一、扩增流程
收到产品后，请先根据产品管壁标签来判断产品形式，并在扩增前准确查找该质粒菌株的抗性、感受态和培养温度。
1、质粒干粉（常温运输，存于-20度，90天保质期，请务必转化挑单克隆培养，不要直接使用和测序）
①收到质粒干粉后请先5000rpm离心1min，再加入20μl ddH2O去离子水溶解质粒；
②取1支100μl 感受态于冰上解冻10min，加入2μl质粒，再冰浴30min后，42℃热激60s，不要搅动，再冰浴2min；（从第二步开始均要在超净工作台中无菌操作）
③加入900μl无抗的LB液体培养基，180rpm震荡37℃培养45min；
④6000rpm离心5min，仅留100μl上清液重悬细菌沉淀，并涂布至目标质粒抗性的LB平板上；（可使用本平台的平板涂布专用玻璃珠进行涂布，可以比传统涂布方法获得更多转化子）
⑤将平板正向培养1h，再倒置37℃培养14h。如果要求是30度则培养20h；
（菌落过多则将质粒稀释后再转化。没有菌落则加入10μl质粒转化。另不要直接转表达感受态，要先转克隆感受态，重提质粒后再导入表达感受态）
⑥挑取单菌落至LB液体培养基中，加入对应抗生素，220rpm震荡培养14h，根据实验需要和质粒提取试剂盒说明书提取质粒。
2、甘油菌种（冰袋运输，存于-80℃，保质期90天，请务必划线挑单克隆培养）
四区划线后挑单菌落培养，酵母菌需要先液体复苏再四区划线，再挑单菌落液体培养。
3、穿刺菌种（冰袋运输，存于4℃，保质期7天）
穿刺接种，液体培养后四区划线，再挑单菌落液体培养。
4、菌落平板（冰袋运输，存于4℃，保质期7天）
直接挑取单菌落至液体培养基中。
5、液体质粒（冰袋运输，存于-20℃，保质期90天）
单独提取的液体质粒收到后可直接使用。
6、滤纸质粒（常温运输，存于-20度，90天保质期，请务必转化挑单克隆培养，不要直接使用和测序）
收到货后将滤纸画圈部分剪下放入EP管中，加100ul无菌水将滤纸浸湿并浸泡5min，吸取5ul质粒转化，离心全涂。
二、转化图片
名称：pAAV2-8腺相关包装质粒
别称: pAAV2-8
| 别名: | 和P13271不一样 |
|---|---|
| 原核抗性: | Amp |
| 克隆菌株: | Stbl3 |
| 培养条件: | 37度 |
质粒属性
| 质粒宿主： | 哺乳细胞,腺相关病毒 |
|---|---|
| 质粒用途： | |
| 片段类型： | |
| 片段物种： | |
| 原核抗性： | Amp |
| 真核抗性： | |
| 荧光标记： |
质粒简介
pAAV2-8质粒由上海烜雅生物科技有限公司提供
质粒图谱
质粒序列
LOCUS Exported 21837 bp ds-DNA circular SYN 04-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..21837
/organism="synthetic DNA construct"
/mol_type="other DNA"
repeat_region 21..123
/label=ITR
/note="inverted terminal repeat of human adenovirus
serotype 5"
misc_signal 211..361
/label=Ad5 Psi
/note="packaging signal for adenovirus serotype 5"
CDS 1346..3211
/codon_start=1
/label=Rep
/translation="MPGFYEIVIKVPSDLDGHLPGISDSFVNWVAEKEWELPPDSDMDL
NLIEQAPLTVAEKLQRDFLTEWRRVSKAPEALFFVQFEKGESYFHMHVLVETTGVKSMV
LGRFLSQIREKLIQRIYRGIEPTLPNWFAVTKTRNGAGGGNKVVDECYIPNYLLPKTQP
ELQWAWTNMEQYLSACLNLTERKRLVAQHLTHVSQTQEQNKENQNPNSDAPVIRSKTSA
RYMELVGWLVDKGITSEKQWIQEDQASYISFNAASNSRSQIKAALDNAGKIMSLTKTAP
DYLVGQQPVEDISSNRIYKILELNGYDPQYAASVFLGWATKKFGKRNTIWLFGPATTGK
TNIAEAIAHTVPFYGCVNWTNENFPFNDCVDKMVIWWEEGKMTAKVVESAKAILGGSKV
RVDQKCKSSAQIDPTPVIVTSNTNMCAVIDGNSTTFEHQQPLQDRMFKFELTRRLDHDF
GKVTKQEVKDFFRWAKDHVVEVEHEFYVKKGGAKKRPAPSDADISEPKRVRESVAQPST
SDAEASINYADRYQNKCSRHVGMNLMLFPCRQCERMNQNSNICFTHGQKDCLECFPVSE
SQPVSVVKKAYQKLCYIHHIMGKVPDACTACDLVNVDLDDCIFEQ"
CDS 3228..5135
/codon_start=1
/label=Cap
/translation="MAADGYLPDWLEDNLSEGIREWWALKPGAPKPKANQQKQDDGRGL
VLPGYKYLGPFNGLDKGEPVNAADAAALEHDKAYDQQLQAGDNPYLRYNHADAEFQERL
QEDTSFGGNLGRAVFQAKKRVLEPLGLVEEGAKTAPGKKRPVEPSPQRSPDSSTGIGKK
GQQPARKRLNFGQTGDSESVPDPQPLGEPPAAPSGVGPNTMAAGGGAPM-ADNNEGADGV
GSSSGNWHCDSTWLGDRVITTSTRTWALPTYNNHLYKQISNGTSGGATNDNTYFGYS-TP
WGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLSFKLFNIQVKEVTQNEGTKTIANNLT
STIQVFTDSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQAVGRSSFYCLE
YFPSQMLRTGNNFQFTYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTQTTGGTA
NTQTLGFSQGGPNTMANQAKNWLPGPCYRQQRVSTTTGQNNNSNFAWTAGTKYHLNGRN
SLANPGIAMATHKDDEERFFPSNGILILANKMLPETMRITAMSCSPA?KKSKPLTLWLQ
RNTVSWQITCSSKTRLLKLELSTARGPYPVWSGRTGTCTCRVPSGPRFLTRTATSTRLR
"
repeat_region 19739..19841
/label=ITR
/note="inverted terminal repeat of human adenovirus
serotype 5"
rep_origin complement(20032..20620)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(20772..21632)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPATMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(21633..21737)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ggatccgaat tcttaattaa catcatcaat aatatacctt attttggatt gaagccaata
61 tgataatgag ggggtggagt ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg
121 tagtagtgtg gcggaagtgt gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg
181 tggcaaaagt gacgtttttg gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg
241 gttttaggcg gatgttgtag taaatttggg cgtaaccgag taagatttgg ccattttcgc
301 gggaaaactg aataagagga agtgaaatct gaataatttt gtgttactca tagcgcgtaa
361 tatttgtcta gggccgcggg gactttgacc gtttacgtgg agactcgccc aggtgttttt
421 ctcaggtgtt ttccgcgttc cgggtcaaag ttggcgtttt attattatag tcagggggat
481 cctctagaac tagtggatcc gtccctcagg cctagaagta aaaaagggaa aaaagagtgt
541 gtttgtcaaa ataggagaca ggtggtggca accaaggact tataggggac cttacatcta
601 cagaccaaca gatgccccct taccatatac aggaagatat gacttaaatt gggataggtg
661 ggtcacaatc aacggctata aagtgttata cagatccctc ccctcccctt tcgtgaaaga
721 ctcgccagag ctagacctcc ttggtgtatg ctaactgaga agagaaagac gacatgaaac
781 aacaggtaca tgattatatt tatctaggaa caggaatgca cttttgggga aaggttttcc
841 ataccaagga aggggcagtg gctggactga tagaacatta ttctgcaaaa acttatggta
901 tgagttatta tgattagcct ttatttgccc aaccttgcgg ttcccagggt ttaaataagt
961 ttatggttac aaactgttct taaaacgagg atgtgagaca agtggtttcc tgacttggtt
1021 tggtaatcaa atgttctgat ctgagctctt agtgttctat tttcctatgt tcttttggaa
1081 tctatccaag tcttatgtaa atgcttatgt aaaccataat ataaaagagt gctgattttt
1141 tgagtaaact tgcaacagtc ctaacattct tctctcgtgt gtttgtgtct gttcgccatc
1201 ccgtctccgc tcgtcactta tccttcactt ttcagagggt ccccccgcag atcccggtca
1261 ccctcaggtc gggacctgca gaagacgccc gagtgagcac gcagggtctc cattttgaag
1321 cgggaggttt gaacgcgcag ccgccatgcc ggggttttac gagattgtga ttaaggtccc
1381 cagcgacctt gacgggcatc tgcccggcat ttctgacagc tttgtgaact gggtggccga
1441 gaaggaatgg gagttgccgc cagattctga catggatctg aatctgattg agcaggcacc
1501 cctgaccgtg gccgagaagc tgcagcgcga ctttctgacg gaatggcgcc gtgtgagtaa
1561 ggccccggag gcccttttct ttgtgcaatt tgagaaggga gagagctact tccacatgca
1621 cgtgctcgtg gaaaccaccg gggtgaaatc catggttttg ggacgtttcc tgagtcagat
1681 tcgcgaaaaa ctgattcaga gaatttaccg cgggatcgag ccgactttgc caaactggtt
1741 cgcggtcaca aagaccagaa atggcgccgg aggcgggaac aaggtggtgg atgagtgcta
1801 catccccaat tacttgctcc ccaaaaccca gcctgagctc cagtgggcgt ggactaatat
1861 ggaacagtat ttaagcgcct gtttgaatct cacggagcgt aaacggttgg tggcgcagca
1921 tctgacgcac gtgtcgcaga cgcaggagca gaacaaagag aatcagaatc ccaattctga
1981 tgcgccggtg atcagatcaa aaacttcagc caggtacatg gagctggtcg ggtggctcgt
2041 ggacaagggg attacctcgg agaagcagtg gatccaggag gaccaggcct catacatctc
2101 cttcaatgcg gcctccaact cgcggtccca aatcaaggct gccttggaca atgcgggaaa
2161 gattatgagc ctgactaaaa ccgcccccga ctacctggtg ggccagcagc ccgtggagga
2221 catttccagc aatcggattt ataaaatttt ggaactaaac gggtacgatc cccaatatgc
2281 ggcttccgtc tttctgggat gggccacgaa aaagttcggc aagaggaaca ccatctggct
2341 gtttgggcct gcaactaccg ggaagaccaa catcgcggag gccatagccc acactgtgcc
2401 cttctacggg tgcgtaaact ggaccaatga gaactttccc ttcaacgact gtgtcgacaa
2461 gatggtgatc tggtgggagg aggggaagat gaccgccaag gtcgtggagt cggccaaagc
2521 cattctcgga ggaagcaagg tgcgcgtgga ccagaaatgc aagtcctcgg cccagataga
2581 cccgactccc gtgatcgtca cctccaacac caacatgtgc gccgtgattg acgggaactc
2641 aacgaccttc gaacaccagc agccgttgca agaccggatg ttcaaatttg aactcacccg
2701 ccgtctggat catgactttg ggaaggtcac caagcaggaa gtcaaagact ttttccggtg
2761 ggcaaaggat cacgtggttg aggtggagca tgaattctac gtcaaaaagg gtggagccaa
2821 gaaaagaccc gcccccagtg acgcagatat aagtgagccc aaacgggtgc gcgagtcagt
2881 tgcgcagcca tcgacgtcag acgcggaagc ttcgatcaac tacgcagaca ggtaccaaaa
2941 caaatgttct cgtcacgtgg gcatgaatct gatgctgttt ccctgcagac aatgcgagag
3001 aatgaatcag aattcaaata tctgcttcac tcacggacag aaagactgtt tagagtgctt
3061 tcccgtgtca gaatctcaac ccgtttctgt cgtcaaaaag gcgtatcaga aactgtgcta
3121 cattcatcat atcatgggaa aggtgccaga cgcttgcact gcctgcgatc tggtcaatgt
3181 ggatttggat gactgcatct ttgaacaata aatgatttaa atcaggtatg gctgccgatg
3241 gttatcttcc agattggctc gaggacaacc tctctgaggg cattcgcgag tggtgggcgc
3301 tgaaacctgg agccccgaag cccaaagcca accagcaaaa gcaggacgac ggccggggtc
3361 tggtgcttcc tggctacaag tacctcggac ccttcaacgg actcgacaag ggggagcccg
3421 tcaacgcggc ggacgcagcg gccctcgagc acgacaaggc ctacgaccag cagctgcagg
3481 cgggtgacaa tccgtacctg cggtataacc acgccgacgc cgagtttcag gagcgtctgc
3541 aagaagatac gtcttttggg ggcaacctcg ggcgagcagt cttccaggcc aagaagcggg
3601 ttctcgaacc tctcggtctg gttgaggaag gcgctaagac ggctcctgga aagaagagac
3661 cggtagagcc atcaccccag cgttctccag actcctctac gggcatcggc aagaaaggcc
3721 aacagcccgc cagaaaaaga ctcaattttg gtcagactgg cgactcagag tcagttccag
3781 accctcaacc tctcggagaa cctccagcag cgccctctgg tgtgggacct aatacaatgg
3841 ctgcaggcgg tggcgcacca atggcagaca ataacgaagg cgccgacgga gtgggtagtt
3901 cctcgggaaa ttggcattgc gattccacat ggctgggcga cagagtcatc accaccagca
3961 cccgaacctg ggccctgccc acctacaaca accacctcta caagcaaatc tccaacggga
4021 catcgggagg agccaccaac gacaacacct acttcggcta cagcaccccc tgggggtatt
4081 ttgactttaa cagattccac tgccactttt caccacgtga ctggcagcga ctcatcaaca
4141 acaactgggg attccggccc aagagactca gcttcaagct cttcaacatc caggtcaagg
4201 aggtcacgca gaatgaaggc accaagacca tcgccaataa cctcaccagc accatccagg
4261 tgtttacgga ctcggagtac cagctgccgt acgttctcgg ctctgcccac cagggctgcc
4321 tgcctccgtt cccggcggac gtgttcatga ttccccagta cggctaccta acactcaaca
4381 acggtagtca ggccgtggga cgctcctcct tctactgcct ggaatacttt ccttcgcaga
4441 tgctgagaac cggcaacaac ttccagttta cttacacctt cgaggacgtg cctttccaca
4501 gcagctacgc ccacagccag agcttggacc ggctgatgaa tcctctgatt gaccagtacc
4561 tgtactactt gtctcggact caaacaacag gaggcacggc aaatacgcag actctgggct
4621 tcagccaagg tgggcctaat acaatggcca atcaggcaaa gaactggctg ccaggaccct
4681 gttaccgcca acaacgcgtc tcaacgacaa ccgggcaaaa caacaatagc aactttgcct
4741 ggactgctgg gaccaaatac catctgaatg gaagaaattc attggctaat cctggcatcg
4801 ctatggcaac acacaaagac gacgaggagc gtttttttcc cagtaacggg atcctgattt
4861 tggcaaacaa aatgctgcca gagacaatgc ggattacagc gatgtcatgc tcaccagcga
4921 gnaagaaatc aaaaccacta accctgtggc tacagaggaa tacggtatcg tggcagataa
4981 cttgcagcag caaaacacgg ctcctcaaat tggaactgtc aacagccagg gggccttacc
5041 cggtatggtc tggcagaacc gggacgtgta cctgcagggt cccatctggg ccaagattcc
5101 tcacacggac ggcaacttcc acccgtctcc gctgatgggc ggctttggcc tgaaacatcc
5161 tccgcctcag atcctgatca agaacacgcc tgtacctgcg gatcctccga ccaccttcaa
5221 ccagtcaaag ctgaactctt tcatcacgca atacagcacc ggacaggtca gcgtggaaat
5281 tgaatgggag ctgcagaagg aaaacagcaa gcgctggaac cccgagatcc agtacacctc
5341 caactactac aaatctacaa gtgtggactt tgctgttaat acagaaggcg tgtactctga
5401 accccgcccc attggcaccc gttacctcac ccgtaatctg taattgcctg ttaatcaata
5461 aaccggttga ttcgtttcag ttgaactttg gtctctgcga agggcgaatt cgtttccatc
5521 gatgcatgtc cttgggtccg gcctgctgaa tgcgcaggcg gtcggccatg ccccaggctt
5581 cgttttgaca tcggcgcagg tctttgtagt agtcttgcat gagcctttct accggcactt
5641 cttcttctcc ttcctcttgt cctgcatctc ttgcatctat cgctgcggcg gcggcggagt
5701 ttggccgtag gtggcgccct cttcctccca tgcgtgtgac cccgaagccc ctcatcggct
5761 gaagcagggc taggtcggcg acaacgcgct cggctaatat ggcctgctgc acctgcgtga
5821 gggtagactg gaagtcatcc atgtccacaa agcggtggta tgcgcccgtg ttgatggtgt
5881 aagtgcagtt ggccataacg gaccagttaa cggtctggtg acccggctgc gagagctcgg
5941 tgtacctgag acgcgagtaa gccctcgagt caaatacgta gtcgttgcaa gtccgcacca
6001 ggtactggta tcccaccaaa aagtgcggcg gcggctggcg gtagaggggc cagcgtaggg
6061 tggccggggc tccgggggcg agatcttcca acataaggcg atgatatccg tagatgtacc
6121 tggacatcca ggtgatgccg gcggcggtgg tggaggcgcg cggaaagtcg cggacgcggt
6181 tccagatgtt gcgcagcggc aaaaagtgct ccatggtcgg gacgctctgg ccggtcaggc
6241 gcgcgcaatc gttgacgctc tagaccgtgc aaaaggagag cctgtaagcg ggcactcttc
6301 cgtggtctgg tggataaatt cgcaagggta tcatggcgga cgaccggggt tcgagccccg
6361 tatccggccg tccgccgtga tccatgcggt taccgcccgc gtgtcgaacc caggtgtgcg
6421 acgtcagaca acgggggagt gctccttttg gcttccttcc aggcgcggcg gctgctgcgc
6481 tagctttttt ggccactggc cgcgcgcagc gtaagcggtt aggctggaaa gcgaaagcat
6541 taagtggctc gctccctgta gccggagggt tattttccaa gggttgagtc gcgggacccc
6601 cggttcgagt ctcggaccgg ccggactgcg gcgaacgggg gtttgcctcc ccgtcatgca
6661 agaccccgct tgcaaattcc tccggaaaca gggacgagcc ccttttttgc ttttcccaga
6721 tgcatccggt gctgcggcag atgcgccccc ctcctcagca gcggcaagag caagagcagc
6781 ggcagacatg cagggcaccc tcccctcctc ctaccgcgtc aggaggggcg acatccgcgg
6841 ttgacgcggc agcagatggt gattacgaac ccccgcggcg ccgggcccgg cactacctgg
6901 acttggagga gggcgagggc ctggcgcggc taggagcgcc ctctcctgag cggtacccaa
6961 gggtgcagct gaagcgtgat acgcgtgagg cgtacgtgcc gcggcagaac ctgtttcgcg
7021 accgcgaggg agaggagccc gaggagatgc gggatcgaaa gttccacgca gggcgcgagc
7081 tgcggcatgg cctgaatcgc gagcggttgc tgcgcgagga ggactttgag cccgacgcgc
7141 gaaccgggat tagtcccgcg cgcgcacacg tggcggccgc cgacctggta accgcatacg
7201 agcagacggt gaaccagggc gatcgcaccc tttggcgcat cccattctcc agtaacttta
7261 tgtccatggg cgcactcaca gacctgggcc aaaaccttct ctacgccaac tccgcccacg
7321 cgctagacat gacttttgag gtggatccca tggacgagcc cacccttctt tatgttttgt
7381 ttgaagtctt tgacgtggtc cgtgtgcacc ggccgcaccg cggcgtcatc gaaaccgtgt
7441 acctgcgcac gcccttctcg gccggcaacg ccacaacata aagaagcaag caacatcaac
7501 aacagctgcc gccatgggct ccagtgagca ggaactgaaa gccattgtca aagatcttgg
7561 ttgtgggcca tattttttgg gcacctatga caagcgcttt ccaggctttg tttctccaca
7621 caagctcgcc tgcgccatag tcaatacggc cggtcgcgag actgggggcg tacactggat
7681 ggcctttgcc tggaacccgc actcaaaaac atgctacctc tttgagccct ttggcttttc
7741 tgaccagcga ctcaagcagg tttaccagtt tgagtacgag tcactcctgc gccgtagcgc
7801 cattgcttct tcccccgacc gctgtataac gctggaaaag tccacccaaa gcgtacaggg
7861 gcccaactcg gccgcctgtg gactattctg ctgcatgttt ctccacgcct ttgccaactg
7921 gccccaaact cccatggatc acaaccccac catgaacctt attaccgggg tacccaactc
7981 catgctcaac agtccccagg tacagcccac cctgcgtcgc aaccaggaac agctctacag
8041 cttcctggag cgccactcgc cctacttccg cagccacagt gcgcagatta ggagcgccac
8101 ttctttttgt cacttgaaaa acatgtaaaa ataatgtact agagacactt tcaataaagg
8161 caaatgcttt tatttgtaca ctctcgggtg attatttacc cccacccttg ccgtctgcgc
8221 cgtttaaaaa tcaaaggggt tctgccgcgc atcgctatgc gccactggca gggacacgtt
8281 gcgatactgg tgtttagtgc tccacttaaa ctcaggcaca accatccgcg gcagctcggt
8341 gaagttttca ctccacaggc tgcgcaccat caccaacgcg tttagcaggt cgggcgccga
8401 tatcttgaag tcgcagttgg ggcctccgcc ctgcgcgcgc gagttgcgat acacagggtt
8461 gcagcactgg aacactatca gcgccgggtg gtgcacgctg gccagcacgc tcttgtcgga
8521 gatcagatcc gcgtccaggt cctccgcgtt gctcagggcg aacggagtca actttggtag
8581 ctgccttccc aaaaagggcg cgtgcccagg ctttgagttg cactcgcacc gtagtggcat
8641 caaaaggtga ccgtgcccgg tctgggcgtt aggatacagc gcctgcataa aagccttgat
8701 ctgcttaaaa gccacctgag cctttgcgcc ttcagagaag aacatgccgc aagacttgcc
8761 ggaaaactga ttggccggac aggccgcgtc gtgcacgcag caccttgcgt cggtgttgga
8821 gatctgcacc acatttcggc cccaccggtt cttcacgatc ttggccttgc tagactgctc
8881 cttcagcgcg cgctgcccgt tttcgctcgt cacatccatt tcaatcacgt gctccttatt
8941 tatcataatg cttccgtgta gacacttaag ctcgccttcg atctcagcgc agcggtgcag
9001 ccacaacgcg cagcccgtgg gctcgtgatg cttgtaggtc acctctgcaa acgactgcag
9061 gtacgcctgc aggaatcgcc ccatcatcgt cacaaaggtc ttgttgctgg tgaaggtcag
9121 ctgcaacccg cggtgctcct cgttcagcca ggtcttgcat acggccgcca gagcttccac
9181 ttggtcaggc agtagtttga agttcgcctt tagatcgtta tccacgtggt acttgtccat
9241 cagcgcgcgc gcagcctcca tgcccttctc ccacgcagac acgatcggca cactcagcgg
9301 gttcatcacc gtaatttcac tttccgcttc gctgggctct tcctcttcct cttgcgtccg
9361 cataccacgc gccactgggt cgtcttcatt cagccgccgc actgtgcgct tacctccttt
9421 gccatgcttg attagcaccg gtgggttgct gaaacccacc atttgtagcg ccacatcttc
9481 tctttcttcc tcgctgtcca cgattacctc tggtgatggc gggcgctcgg gcttgggaga
9541 agggcgcttc tttttcttct tgggcgcaat ggccaaatcc gccgccgagg tcgatggccg
9601 cgggctgggt gtgcgcggca ccagcgcgtc ttgtgatgag tcttcctcgt cctcggactc
9661 gatacgccgc ctcatccgct tttttggggg cgcccgggga ggcggcggcg acggggacgg
9721 ggacgacacg tcctccatgg ttgggggacg tcgcgccgca ccgcgtccgc gctcgggggt
9781 ggtttcgcgc tgctcctctt cccgactggc catttccttc tcctataggc agaaaaagat
9841 catggagtca gtcgagaaga aggacagcct aaccgccccc tctgagttcg ccaccaccgc
9901 ctccaccgat gccgccaacg cgcctaccac cttccccgtc gaggcacccc cgcttgagga
9961 ggaggaagtg attatcgagc aggacccagg ttttgtaagc gaagacgacg aggaccgctc
10021 agtaccaaca gaggataaaa agcaagacca ggacaacgca gaggcaaacg aggaacaagt
10081 cgggcggggg gacgaaaggc atggcgacta cctagatgtg ggagacgacg tgctgttgaa
10141 gcatctgcag cgccagtgcg ccattatctg cgacgcgttg caagagcgca gcgatgtgcc
10201 cctcgccata gcggatgtca gccttgccta cgaacgccac ctattctcac cgcgcgtacc
10261 ccccaaacgc caagaaaacg gcacatgcga gcccaacccg cgcctcaact tctaccccgt
10321 atttgccgtg ccagaggtgc ttgccaccta tcacatcttt ttccaaaact gcaagatacc
10381 cctatcctgc cgtgccaacc gcagccgagc ggacaagcag ctggccttgc ggcagggcgc
10441 tgtcatacct gatatcgcct cgctcaacga agtgccaaaa atctttgagg gtcttggacg
10501 cgacgagaag cgcgcggcaa acgctctgca acaggaaaac agcgaaaatg aaagtcactc
10561 tggagtgttg gtggaactcg agggtgacaa cgcgcgccta gccgtactaa aacgcagcat
10621 cgaggtcacc cactttgcct acccggcact taacctaccc cccaaggtca tgagcacagt
10681 catgagtgag ctgatcgtgc gccgtgcgca gcccctggag agggatgcaa atttgcaaga
10741 acaaacagag gagggcctac ccgcagttgg cgacgagcag ctagcgcgct ggcttcaaac
10801 gcgcgagcct gccgacttgg aggagcgacg caaactaatg atggccgcag tgctcgttac
10861 cgtggagctt gagtgcatgc agcggttctt tgctgacccg gagatgcagc gcaagctaga
10921 ggaaacattg cactacacct ttcgacaggg ctacgtacgc caggcctgca agatctccaa
10981 cgtggagctc tgcaacctgg tctcctacct tggaattttg cacgaaaacc gccttgggca
11041 aaacgtgctt cattccacgc tcaagggcga ggcgcgccgc gactacgtcc gcgactgcgt
11101 ttacttattt ctatgctaca cctggcagac ggccatgggc gtttggcagc agtgcttgga
11161 ggagtgcaac ctcaaggagc tgcagaaact gctaaagcaa aacttgaagg acctatggac
11221 ggccttcaac gagcgctccg tggccgcgca cctggcggac atcattttcc ccgaacgcct
11281 gcttaaaacc ctgcaacagg gtctgccaga cttcaccagt caaagcatgt tgcagaactt
11341 taggaacttt atcctagagc gctcaggaat cttgcccgcc acctgctgtg cacttcctag
11401 cgactttgtg cccattaagt accgcgaatg ccctccgccg ctttggggcc actgctacct
11461 tctgcagcta gccaactacc ttgcctacca ctctgacata atggaagacg tgagcggtga
11521 cggtctactg gagtgtcact gtcgctgcaa cctatgcacc ccgcaccgct ccctggtttg
11581 caattcgcag ctgcttaacg aaagtcaaat tatcggtacc tttgagctgc agggtccctc
11641 gcctgacgaa aagtccgcgg ctccggggtt gaaactcact ccggggctgt ggacgtcggc
11701 ttaccttcgc aaatttgtac ctgaggacta ccacgcccac gagattaggt tctacgaaga
11761 ccaatcccgc ccgccaaatg cggagcttac cgcctgcgtc attacccagg gccacattct
11821 tggccaattg caagccatca acaaagcccg ccaagagttt ctgctacgaa agggacgggg
11881 ggtttacttg gacccccagt ccggcgagga gctcaaccca atccccccgc cgccgcagcc
11941 ctatcagcag cagccgcggg cccttgcttc ccaggatggc acccaaaaag aagctgcagc
12001 tgccgccgcc acccacggac gaggaggaat actgggacag tcaggcagag gaggttttgg
12061 acgaggagga ggaggacatg atggaagact gggagagcct agacgaggaa gcttccgagg
12121 tcgaagaggt gtcagacgaa acaccgtcac cctcggtcgc attcccctcg ccggcgcccc
12181 agaaatcggc aaccggttcc agcatggcta caacctccgc tcctcaggcg ccgccggcac
12241 tgcccgttcg ccgacccaac cgtagatggg acaccactgg aaccagggcc ggtaagtcca
12301 agcagccgcc gccgttagcc caagagcaac aacagcgcca aggctaccgc tcatggcgcg
12361 ggcacaagaa cgccatagtt gcttgcttgc aagactgtgg gggcaacatc tccttcgccc
12421 gccgctttct tctctaccat cacggcgtgg ccttcccccg taacatcctg cattactacc
12481 gtcatctcta cagcccatac tgcaccggcg gcagcggcag cggcagcaac agcagcggcc
12541 acacagaagc aaaggcgacc ggatagcaag actctgacaa agcccaagaa atccacagcg
12601 gcggcagcag caggaggagg agcgctgcgt ctggcgccca acgaacccgt atcgacccgc
12661 gagcttagaa acaggatttt tcccactctg tatgctatat ttcaacagag caggggccaa
12721 gaacaagagc tgaaaataaa aaacaggtct ctgcgatccc tcacccgcag ctgcctgtat
12781 cacaaaagcg aagatcagct tcggcgcacg ctggaagacg cggaggctct cttcagtaaa
12841 tactgcgcgc tgactcttaa ggactagttt cgcgcccttt ctcaaattta agcgcgaaaa
12901 ctacgtcatc tccagcggcc acacccggcg ccagcacctg tcgtcagcgc cattatgagc
12961 aaggaaattc ccacgcccta catgtggagt taccagccac aaatgggact tgcggctgga
13021 gctgcccaag actactcaac ccgaataaac tacatgagcg cgggacccca catgatatcc
13081 cgggtcaacg gaatccgcgc ccaccgaaac cgaattctct tggaacaggc ggctattacc
13141 accacacctc gtaataacct taatccccgt agttggcccg ctgccctggt gtaccaggaa
13201 agtcccgctc ccaccactgt ggtacttccc agagacgccc aggccgaagt tcagatgact
13261 aactcagggg cgcagcttgc gggcggcttt cgtcacaggg tgcggtcgcc cgggcagggt
13321 ataactcacc tgacaatcag agggcgaggt attcagctca acgacgagtc ggtgagctcc
13381 tcgcttggtc tccgtccgga cgggacattt cagatcggcg gcgccggccg ctcttcattc
13441 acgcctcgtc aggcaatcct aactctgcag acctcgtcct ctgagccgcg ctctggaggc
13501 attggaactc tgcaatttat tgaggagttt gtgccatcgg tctactttaa ccccttctcg
13561 ggacctcccg gccactatcc ggatcaattt attcctaact ttgacgcggt aaaggactcg
13621 gcggatggct acgactgaat gttaagtgga gaggcagagc aactgcgcct gaaacacctg
13681 gtccactgtc gccgccacaa gtgctttgcc cgcgactccg gtgagttttg ctactttgaa
13741 ttgcccgagg atcatatcga gggcccggcg cacggcgtcc ggcttaccgc ccagggagag
13801 cttgcccgta gcctgattcg ggagtttacc cagcgccccc tgctagttga gcgggacagg
13861 ggaccctgtg ttctcactgt gatttgcaac tgtcctaacc ctggattaca tcaagatctt
13921 tgttgccatc tctgtgctga gtataataaa tacagaaatt aaaatatact ggggctccta
13981 tcgccatcct gtaaacgcca ccgtcttcac ccgcccaagc aaaccaaggc gaaccttacc
14041 tggtactttt aacatctctc cctctgtgat ttacaacagt ttcaacccag acggagtgag
14101 tctacgagag aacctctccg agctcagcta ctccatcaga aaaaacacca ccctccttac
14161 ctgccgggaa cgtacgagtg cgtcaccggc cgctgcacca cacctaccgc ctgaccgtaa
14221 accagacttt ttccggacag acctcaataa ctctgtttac cagaacagga ggtgagctta
14281 gaaaaccctt agggtattag gccaaaggcg cagctactgt ggggtttatg aacaattcaa
14341 gcaactctac gggctattct aattcaggtt tctctagaaa tggacggaat tattacagag
14401 cagcgcctgc tagaaagacg cagggcagcg gccgagcaac agcgcatgaa tcaagagctc
14461 caagacatgg ttaacttgca ccagtgcaaa aggggtatct tttgtctggt aaagcaggcc
14521 aaagtcacct acgacagtaa taccaccgga caccgcctta gctacaagtt gccaaccaag
14581 cgtcagaaat tggtggtcat ggtgggagaa aagcccatta ccataactca gcactcggta
14641 gaaaccgaag gctgcattca ctcaccttgt caaggacctg aggatctctg cacccttatt
14701 aagaccctgt gcggtctcaa agatcttatt ccctttaact aataaaaaaa aataataaag
14761 catcacttac ttaaaatcag ttagcaaatt tctgtccagt ttattcagca gcacctcctt
14821 gccctcctcc cagctctggt attgcagctt cctcctggct gcaaactttc tccacaatct
14881 aaatggaatg tcagtttcct cctgttcctg tccatccgca cccactatct tcatgttgtt
14941 gcagatgaag cgcgcaagac cgtctgaaga taccttcaac cccgtgtatc catatgacac
15001 ggaaaccggt cctccaactg tgccttttct tactcctccc tttgtatccc ccaatgggtt
15061 tcaagagagt ccccctgggg tactctcttt gcgcctatcc gaacctctag ttacctccaa
15121 tggcatgctt gcgctcaaaa tgggcaacgg cctctctctg gacgaggccg gcaaccttac
15181 ctcccaaaat gtaaccactg tgagcccacc tctcaaaaaa accaagtcaa acataaacct
15241 ggaaatatct gcacccctca cagttacctc agaagcccta actgtggctg ccgccgcacc
15301 tctaatggtc gcgggcaaca cactcaccat gcaatcacag gccccgctaa ccgtgcacga
15361 ctccaaactt agcattgcca cccaaggacc cctcacagtg tcagaaggaa agctagccct
15421 gcaaacatca ggccccctca ccaccaccga tagcagtacc cttactatca ctgcctcacc
15481 ccctctaact actgccactg gtagcttggg cattgacttg aaagagccca tttatacaca
15541 aaatggaaaa ctaggactaa agtacggggc tcctttgcat gtaacagacg acctaaacac
15601 tttgaccgta gcaactggtc caggtgtgac tattaataat acttccttgc aaactaaagt
15661 tactggagcc ttgggttttg attcacaagg caatatgcaa cttaatgtag caggaggact
15721 aaggattgat tctcaaaaca gacgccttat acttgatgtt agttatccgt ttgatgctca
15781 aaaccaacta aatctaagac taggacaggg ccctcttttt ataaactcag cccacaactt
15841 ggatattaac tacaacaaag gcctttactt gtttacagct tcaaacaatt ccaaaaagct
15901 tgaggttaac ctaagcactg ccaaggggtt gatgtttgac gctacagcca tagccattaa
15961 tgcaggagat gggcttgaat ttggttcacc taatgcacca aacacaaatc ccctcaaaac
16021 aaaaattggc catggcctag aatttgattc aaacaaggct atggttccta aactaggaac
16081 tggccttagt tttgacagca caggtgccat tacagtagga aacaaaaata atgataagct
16141 aactttgtgg accacaccag ctccatctcc taactgtaga ctaaatgcag agaaagatgc
16201 taaactcact ttggtcttaa caaaatgtgg cagtcaaata cttgctacag tttcagtttt
16261 ggctgttaaa ggcagtttgg ctccaatatc tggaacagtt caaagtgctc atcttattat
16321 aagatttgac gaaaatggag tgctactaaa caattccttc ctggacccag aatattggaa
16381 ctttagaaat ggagatctta ctgaaggcac agcctataca aacgctgttg gatttatgcc
16441 taacctatca gcttatccaa aatctcacgg taaaactgcc aaaagtaaca ttgtcagtca
16501 agtttactta aacggagaca aaactaaacc tgtaacacta accattacac taaacggtac
16561 acaggaaaca ggagacacaa ctccaagtgc atactctatg tcattttcat gggactggtc
16621 tggccacaac tacattaatg aaatatttgc cacatcctct tacacttttt catacattgc
16681 ccaagaataa agaatcgttt gtgttatgtt tcaacgtgtt tatttttcaa ttgcagaaaa
16741 tttcaagtca tttttcattc agtagtatag ccccaccacc acatagctta tacagatcac
16801 cgtaccttaa tcaaactcac agaaccctag tattcaacct gccacctccc tcccaacaca
16861 cagagtacac agtcctttct ccccggctgg ccttaaaaag catcatatca tgggtaacag
16921 acatattctt aggtgttata ttccacacgg tttcctgtcg agccaaacgc tcatcagtga
16981 tattaataaa ctccccgggc agctcactta agttcatgtc gctgtccagc tgctgagcca
17041 caggctgctg tccaacttgc ggttgcttaa cgggcggcga aggagaagtc cacgcctaca
17101 tgggggtaga gtcataatcg tgcatcagga tagggcggtg gtgctgcagc agcgcgcgaa
17161 taaactgctg ccgccgccgc tccgtcctgc aggaatacaa catggcagtg gtctcctcag
17221 cgatgattcg caccgcccgc agcataaggc gccttgtcct ccgggcacag cagcgcaccc
17281 tgatctcact taaatcagca cagtaactgc agcacagcac cacaatattg ttcaaaatcc
17341 cacagtgcaa ggcgctgtat ccaaagctca tggcggggac cacagaaccc acgtggccat
17401 cataccacaa gcgcaggtag attaagtggc gacccctcat aaacacgctg gacataaaca
17461 ttacctcttt tggcatgttg taattcacca cctcccggta ccatataaac ctctgattaa
17521 acatggcgcc atccaccacc atcctaaacc agctggccaa aacctgcccg ccggctatac
17581 actgcaggga accgggactg gaacaatgac agtggagagc ccaggactcg taaccatgga
17641 tcatcatgct cgtcatgata tcaatgttgg cacaacacag gcacacgtgc atacacttcc
17701 tcaggattac aagctcctcc cgcgttagaa ccatatccca gggaacaacc cattcctgaa
17761 tcagcgtaaa tcccacactg cagggaagac ctcgcacgta actcacgttg tgcattgtca
17821 aagtgttaca ttcgggcagc agcggatgat cctccagtat ggtagcgcgg gtttctgtct
17881 caaaaggagg tagacgatcc ctactgtacg gagtgcgccg agacaaccga gatcgtgttg
17941 gtcgtagtgt catgccaaat ggaacgccgg acgtagtcat atttcctgaa gcaaaaccag
18001 gtgcgggcgt gacaaacaga tctgcgtctc cggtctcgcc gcttagatcg ctctgtgtag
18061 tagttgtagt atatccactc tctcaaagca tccaggcgcc ccctggcttc gggttctatg
18121 taaactcctt catgcgccgc tgccctgata acatccacca ccgcagaata agccacaccc
18181 agccaaccta cacattcgtt ctgcgagtca cacacgggag gagcgggaag agctggaaga
18241 accatgtttt tttttttatt ccaaaagatt atccaaaacc tcaaaatgaa gatctattaa
18301 gtgaacgcgc tcccctccgg tggcgtggtc aaactctaca gccaaagaac agataatggc
18361 atttgtaaga tgttgcacaa tggcttccaa aaggcaaacg gccctcacgt ccaagtggac
18421 gtaaaggcta aacccttcag ggtgaatctc ctctataaac attccagcac cttcaaccat
18481 gcccaaataa ttctcatctc gccaccttct caatatatct ctaagcaaat cccgaatatt
18541 aagtccggcc attgtaaaaa tctgctccag agcgccctcc accttcagcc tcaagcagcg
18601 aatcatgatt gcaaaaattc aggttcctca cagacctgta taagattcaa aagcggaaca
18661 ttaacaaaaa taccgcgatc ccgtaggtcc cttcgcaggg ccagctgaac ataatcgtgc
18721 aggtctgcac ggaccagcgc ggccacttcc ccgccaggaa ccatgacaaa agaacccaca
18781 ctgattatga cacgcatact cggagctatg ctaaccagcg tagccccgat gtaagcttgt
18841 tgcatgggcg gcgatataaa atgcaaggtg ctgctcaaaa aatcaggcaa agcctcgcgc
18901 aaaaaagaaa gcacatcgta gtcatgctca tgcagataaa ggcaggtaag ctccggaacc
18961 accacagaaa aagacaccat ttttctctca aacatgtctg cgggtttctg cataaacaca
19021 aaataaaata acaaaaaaac atttaaacat tagaagcctg tcttacaaca ggaaaaacaa
19081 cccttataag cataagacgg actacggcca tgccggcgtg accgtaaaaa aactggtcac
19141 cgtgattaaa aagcaccacc gacagctcct cggtcatgtc cggagtcata atgtaagact
19201 cggtaaacac atcaggttga ttcacatcgg tcagtgctaa aaagcgaccg aaatagcccg
19261 ggggaataca tacccgcagg cgtagagaca acattacagc ccccatagga ggtataacaa
19321 aattaatagg agagaaaaac acataaacac ctgaaaaacc ctcctgccta ggcaaaatag
19381 caccctcccg ctccagaaca acatacagcg cttccacagc ggcagccata acagtcagcc
19441 ttaccagtaa aaaagaaaac ctattaaaaa aacaccactc gacacggcac cagctcaatc
19501 agtcacagtg taaaaaaggg ccaagtgcag agcgagtata tataggacta aaaaatgacg
19561 taacggttaa agtccacaaa aaacacccag aaaaccgcac gcgaacctac gcccagaaac
19621 gaaagccaaa aaacccacaa cttcctcaaa tcgtcacttc cgttttccca cgttacgtca
19681 cttcccattt taagaaaact acaattccca acacatacaa gttactccgc cctaaaacct
19741 acgtcacccg ccccgttccc acgccccgcg ccacgtcaca aactccaccc cctcattatc
19801 atattggctt caatccaaaa taaggtatat tattgatgat gttaattaag aattcggatc
19861 tgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg
19921 gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct
19981 tcacggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
20041 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
20101 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
20161 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
20221 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
20281 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
20341 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
20401 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
20461 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
20521 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
20581 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
20641 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
20701 atcaaaaagg atcttcacct agatcctttt aaatcaatct aaagtatata tgagtaaact
20761 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
20821 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
20881 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
20941 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
21001 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
21061 agtttgcgca acgttgttgc cattgttgca ggcatcgtgg tgtcacgctc gtcgtttggt
21121 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
21181 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
21241 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
21301 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
21361 cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact
21421 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
21481 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
21541 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
21601 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
21661 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
21721 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
21781 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaa
//
质粒菌株产品操作说明书
一、扩增流程
收到产品后，请先根据产品管壁标签来判断产品形式，并在扩增前准确查找该质粒菌株的抗性、感受态和培养温度。
1、质粒干粉（常温运输，存于-20度，90天保质期，请务必转化挑单克隆培养，不要直接使用和测序）
①收到质粒干粉后请先5000rpm离心1min，再加入20μl ddH2O去离子水溶解质粒；
②取1支100μl 感受态于冰上解冻10min，加入2μl质粒，再冰浴30min后，42℃热激60s，不要搅动，再冰浴2min；（从第二步开始均要在超净工作台中无菌操作）
③加入900μl无抗的LB液体培养基，180rpm震荡37℃培养45min；
④6000rpm离心5min，仅留100μl上清液重悬细菌沉淀，并涂布至目标质粒抗性的LB平板上；（可使用本平台的平板涂布专用玻璃珠进行涂布，可以比传统涂布方法获得更多转化子）
⑤将平板正向培养1h，再倒置37℃培养14h。如果要求是30度则培养20h；
（菌落过多则将质粒稀释后再转化。没有菌落则加入10μl质粒转化。另不要直接转表达感受态，要先转克隆感受态，重提质粒后再导入表达感受态）
⑥挑取单菌落至LB液体培养基中，加入对应抗生素，220rpm震荡培养14h，根据实验需要和质粒提取试剂盒说明书提取质粒。
2、甘油菌种（冰袋运输，存于-80℃，保质期90天，请务必划线挑单克隆培养）
四区划线后挑单菌落培养，酵母菌需要先液体复苏再四区划线，再挑单菌落液体培养。
3、穿刺菌种（冰袋运输，存于4℃，保质期7天）
穿刺接种，液体培养后四区划线，再挑单菌落液体培养。
4、菌落平板（冰袋运输，存于4℃，保质期7天）
直接挑取单菌落至液体培养基中。
5、液体质粒（冰袋运输，存于-20℃，保质期90天）
单独提取的液体质粒收到后可直接使用。
6、滤纸质粒（常温运输，存于-20度，90天保质期，请务必转化挑单克隆培养，不要直接使用和测序）
收到货后将滤纸画圈部分剪下放入EP管中，加100ul无菌水将滤纸浸湿并浸泡5min，吸取5ul质粒转化，离心全涂。
二、转化图片
| P4475/pCMV-SPORT6-HLA-DPB1人源基因质粒 |
| P4476/pCMV-SPORT6-TMEM263人源基因质粒 |
| P4477/pCMV-SPORT6-CDC42SE1人源基因质粒 |
| P4478/pCMV-SPORT6-EFNA3人源基因质粒 |
| P4479/pCMV-SPORT6-CHCHD1人源基因质粒 |
| P4480/pCMV-SPORT6-ARL11人源基因质粒 |
| P4481/pCMV-SPORT6-IFI27人源基因质粒 |
| P4482/pCMV-SPORT6-RTP4人源基因质粒 |
| P4483/pCMV-SPORT6-DCUN1D1人源基因质粒 |
| P4484/pCMV-SPORT6-PDZK1IP1人源基因质粒 |
| P4485/pCMV-SPORT6-RPL32人源基因质粒 |
| P4486/pCMV-SPORT6-DNAJC25-GNG10人源基因质粒 |
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文献和实验相关实验
信息。慢病毒包装质粒可提供所有的转录并包装 RNA 到重组的假病毒载体所需要的所有辅助蛋白。 为产生高滴度的病毒颗粒，需要利用表达载体和包装质粒同时共转染细胞，在细胞中进行病毒的包装，包装好的假病毒颗粒分泌到细胞外的培养基中，离心取得上清液后，可以直接用于宿主细胞的感染，目的基因进入到宿主细胞之后，经过反转录，整合到基因组，从而高水平的表达效应分子。 二、这一系统的目的，主要是为了解决以下问题： 对于一些较难转染的细胞，如原代细胞、干细胞、不分化的细胞等，能大大提高目的基因转导效率，而且目的
毒载体（ Lentiviral vector ）较逆转录病毒载体有更广的宿主范围，慢病毒能够有效感染非周期性和有丝分裂后的细胞。慢病毒载体能够产生表达 shRNA 的高滴度的慢病毒，在周期性和非周期性细胞、干细胞、受精卵以及分化的后代细胞中表达 shRNA ，实现在多种类型的细胞和转基因小鼠中特异而稳定的基因表达的功能性沉默，为在原代的人和动物细胞组织中快速而高效地研究基因功能，以及产生特定基因表达降低的动物提供了可能性。 慢病毒表达载体包含了包装、转染、稳定整合所需要的遗传信息。慢病毒包装质粒可提供所有的转录并包装
摘要: 腺相关病毒(AAV)是一种人细小病毒，目前因为能作为一种基因治疗载体而受到广泛关注。构建重组AAV(rAAV)涉及到用一个目的基因替换病毒基因组的大部分，然后将这个重组基因组包装到一个有感染性的病毒颗粒里。目前大多数生成rAAV的实验方案需要共转染一个载体质粒和一个表达病毒复制和结构基因的包装质粒到腺病毒(Ad)感染的培养细胞中。此方法的局限性包括(1) Ad辅助病毒污染rAAV，(2)rAAV的低产出，(3)生成有复制能力的AAV
pAAV2-8腺相关包装质粒
￥100 - 1000
