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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
见详情说明
- 保质期:
见详情说明
- 库存:
50
- 供应商:
博尔森生物
- 规格:
100 mL
| DiscoverX 提供具有化学发光和荧光读数的检测试剂,以满足您的药物发现计划的不同需求。DiscoverX 的化学发光检测基于专有的酶片段互补 (EFC) 技术,而荧光检测基于激活的荧光染料。这些检测试剂针对各种应用进行了优化,例如检测基于细胞的蛋白质-蛋白质相互作用、化合物-蛋白质结合、易位和第二信使测定。 DiscoverX 的检测试剂专为 DiscoverX 细胞系设计,包括 cAMP Hunter™、InCELL Hunter™ 或 PathHunter® 细胞系、InCELL 检测和 ProLabel®/ProLink™ (PL/PK) 表达或全细胞内的克隆载体。 |
产品亮点
- 灵敏的检测和可重现的结果提供您可以信赖的可靠检测
- 简单的一步添加方案,非常适合多种基础研究和药物发现应用
- 可从 96 孔扩展到 2867 孔格式,与高通量筛选或小型化兼容
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文献和实验Isolation of Mutagen-Sensitive Chinese Hamster Cell Lines by Replica Plating
Cell lines with an increased sensitivity to mutagens, such as ultraviolet (UV) light, X-rays, alkylating compounds, and crosslinking agents, are defective in a cellular response to these agents. These responses include mechanisms that process
The MTT Cell Proliferation Assay
Cell number per well too high. Decrease cell density at plating.Contamination of culture with bacteria or yeast. Remedy Discard. View wells prior to addition of MTT Reagent to check for contamination. 4、Problem: Absorbance readings are too low
,and the cell pellets were frozen and stored at –80ºC. Lysis Lysis was accomplished with B-PER reagent supplemented with 1/50 vol 10-mg/ml chicken egg-white lysozyme (stored at –80ºC in aliquots),1/125 vol 5-mg/ml DNaseI (in 20 mM sodium acetate,pH adjusted
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