- ￥3701
- Megazyme
- 爱尔兰
- E-MALTS
- 2025年12月14日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保质期：
> 4 years at 4oC.
- 英文名：
α-Glucosidase (yeast maltase)
- 库存：
请咨询
- 供应商：
上海经科化学科技有限公司
- 保存条件：
2-8℃
- 规格：
2000 Units
alpha-葡萄糖苷酶alpha-Glucosidase介绍：
中文名：α-葡萄糖苷酶[酵母麦芽糖酶]
英文名：α-Glucosidase (yeast maltase)
货号：E-MALTS
CAS:9001-42-7
规格：2000 Units
价格：3701元
类别：碳水化合物活性酶
High purity α-Glucosidase (yeast maltase) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
| Content: | 2,000 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 1 year under recommended storage conditions |
| Enzyme Activity: | α-Glucosidase |
| EC Number: | 3.2.1.20 |
| CAZy Family: | GH13 |
| CAS Number: | 9001-42-7 |
| Synonyms: | alpha-glucosidase; alpha-D-glucoside glucohydrolase |
| Source: | Yeast |
| Molecular Weight: | 52,000 |
| Concentration: | Supplied at ~ 1,000 U/mL |
| Expression: | From Yeast |
| Specificity: | Hydrolysis of terminal, non-reducing (1,4)-linked α-D-glucose residues with release of D-glucose. |
| Specific Activity: | ~ 120 U/mg (40oC, pH 6.8 on pNP-α-Glucosidase) |
| Unit Definition: | One Unit of α-glucosidase activity is defined as the amount of enzyme required to produce one µmole of p-nitrophenol from pNP-α-Glucosidase (10 mM) in sodium phosphate buffer (100 mM), pH 6.8 at 40oC. |
| Temperature Optima: | 40oC |
| pH Optima: | 6.8 |
| Application examples: | Applications in carbohydrate research and in the food and feeds, brewing and biofuels industries. |
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- 作者
- 内容
- 询问日期
文献和实验1.Development and validation of two analytical strategies for the determination of glucosides of acidic herbicides in cereals and oilseed matrices.
Aloisi, I. & Mol, H. (2023). Analytical and Bioanalytical Chemistry, 1-11.
2.Preliminary Phytochemical Screening, In Vitro Antidiabetic, Antioxidant Activities, and Toxicity of Leaf Extracts of Psychotria malayana Jack.
Nipun, T. S., K-hatib, A., Ahmed, Q. U., Nasir, M. H. M., Supandi, F., Taher, M. & Saiman, M. Z. (2021). Plants, 10(12), 2688.
3.Diurnal patterns of growth and transient reserves of sink and source tissues are affected by cold nights in barley.
Barros, K. A., Esteves‐Ferreira, A. A., Inaba, M., Meally, H., Finnan, J., Barth, S., Davis, S. J. & Sulpice, R. (2020). Plant, Cell & Environment, 43(6), 1404-1420.
4.Identification of α-glucosidase inhibitors from Clinacanthus nutans leaf extract using liquid chromatography-mass spectrometry-based metabolomics and protein-ligand interaction with molecular docking.
Murugesu, S., Ibrahim, Z., Ahmed, Q. U., Uzir, B. F., Yusoff, N. I. N., Perumal, V., Saari, K., K-hatib, A. & K-hatib, A. (2019). Journal of Pharmaceutical Analysis, 9(2), 91-99.
5.Rapid investigation of α-glucosidase inhibitory activity of Clinacanthus nutans leaf using infrared fingerprinting.
Murugesu, S., Ahmed, Q. U., Uzir, B. F., Yusoff, N. I. N., Perumal, V., Ibrahim, Z., Abas, F., Saari, K. & K-hatib, A. (2019). Vibrational Spectroscopy, 100, 22-29.
Revanna, R., Turnbull, M. H., Shaw, M. L., Wright, K. M., Butler, R. C., Jameson, P. E. & McCallum, J. A. (2013). Journal of the Science of Food and Agriculture, 93(10), 2470-2477.
原来是对可使麦芽糖水解生成2分子葡萄糖的酶所用的名称，但现在一般地是作为作用于结合各种配糖基的α -D-葡萄糖苷的α -葡萄糖苷酶的别名来使用。
缺乏维生素 E，就会阻碍受精卵在子宫内着床后的发育，可使精细胞变性，发生退化，引起不孕症。此外，还能引起肌肉萎缩和运动麻痹。在许多哺乳动物中都可见到 E缺乏症，但在人体中，还没有得到确实的 E缺乏症的证据，一般在生物体内有防止维生素 A、胡萝卜素、脂肪等氧化的作用。亦有报道其磷酸脂可抑制琥珀酸氧化系统和抑制磷酸酶的作用，但都还未最后确定。成人每天需要量约为 30毫克的混合生育酚。定量法有吸收测定和比色法等，但因而为α，β，γ、δ都作为同一效力被测定，所以测定数值比生物学效力高，所以并不是太好的方法。
E.Z.N.A.® Yeast Plasmid Mini Spin Protocol
实验步骤 1. Inoculate 5 ml YPD medium placed in a 10-20 ml culture tube with Yeast carrying desired plasmid and grow at 30°C with agitation for 20-24 h. 2. Pellet 1-3 ml yeast culture (use 7 cells) by centrifugation at 4,000×g
