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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保质期：
> 4 years at 4oC
- 英文名：
endo-1,3-β-Glucanase (Trichoderma sp.)
- 库存：
请咨询
- 供应商：
上海经科化学科技有限公司
- 保存条件：
2-8℃
- 规格：
100 Units
1,3-β-D-葡聚糖内切酶endo-1,3-β-D-Glucanase (Trichodermasp.)介绍：
中文名称：1,3-β-D-葡聚糖内切酶[木霉菌]
英文名称：endo-1,3-β-D-Glucanase (Trichodermasp.)
货号：E-LAMSE
规格：100 Units
价格：3023元
类别：碳水化合物活性酶
简介：碳水化合物活性酶，活跃在复杂的碳水化合物和糖复合物，
High purity endo-1,3(4)-β-D-Glucanase (Trichoderma sp.) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
| Content: | 100 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 1 year under recommended storage conditions |
| Enzyme Activity: | endo-1,3(4)-β-Glucanase |
| EC Number: | 3.2.1.6 |
| CAZy Family: | GH16 |
| CAS Number: | 62213-14-3 |
| Synonyms: | glucan endo-1,3(4)-beta-D-glucosidase |
| Source: | Trichoderma sp. |
| Molecular Weight: | 32,000 |
| Concentration: | Supplied at ~ 50 U/mL |
| Expression: | Purified from Trichoderma sp. |
| Specificity: | Hydrolysis of (1,3)-β-D-glucosidic linkages in (1,3)-β-D Glucans and (1,3)(1,4)-β-D-glucosidic linkages in mixed linkage β-D-Glucans. |
| Specific Activity: | > 10 U/mg (40oC, pH 4.5 on CM-Curdlan) |
| Unit Definition: | One Unit of endo-1,3-β-D-Glucanase activity is defined as the amount of enzyme required to release one µmole of glucose-reducing-sugar equivalents per minute in the presence of CM-Curdlan (5 mg/mL) in sodium acetate buffer (200 mM) at pH 4.5 and 40oC. |
| Temperature Optima: | 40oC |
| pH Optima: | 4.5 |
| Application examples: | Applications in carbohydrate and biofuels research and in the food and feeds industries. |
1,3-β-D-葡聚糖内切酶endo-1,3-β-D-Glucanase (Trichodermasp.)说明书(PDF)请向客服索取！
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文献和实验1.Structural and mechanistic insights into fungal β-1, 3-glucan synthase FKS1.
Hu, X., Yang, P., Chai, C., Liu, J., Sun, H., Wu, Y., Zhang, M., Zhang, M, Liu, X. & Yu, H. (2023). Nature, 38, 1-9.
2.Topography of UV-melanized Thalli of Lobaria pulmonaria (L.) Hoffm.
Daminova, A. G., Rassabina, A. E., Khabibrakhmanova, V. R., Beckett, R. P. & Minibayeva, F. V. (2023). Plants (Basel), 12(14), 2627.
3.Properties of corn-expressed carbohydrase AC1 in swine diets and its effects on apparent ileal digestibility, performance, hematology, and serum chemistry.
Lessard, P. A., Li, X., Broomhead, J. N., Parker, M. H., Bailey, C. & Raab, R. M. (2021). Heliyon, 7(8), e07696.
Minoia, S., Boualem, A., Marcel, F., Troadec, C., Quemener, B., Cellini, F., Petrozza, A., Vigouroux, J., Lahaye, M., Carriero, F. & Bendahmane, A. (2016). Plant Science, 242, 195-202.
5.Regulation of the cellulose synthase-like gene family by light in the maize mesocotyl.
Van Erp, H. & Walton, J. D. (2009). Planta, 229(4), 885-897.
Strategies to Optimize Protein Expression in E. coli
. Butt, T.R., Jonnalagadda, S., Monia, B.P., Sternberg, E.J., Marsh, J.A., Stadel, J.M., Ecker, D.J., and Crooke, S.T. 1989. Ubiquitin fusion augments the yield of cloned gene‐products in Escherichia coli. Proc
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pGEM-3Z ( 2.74kb, T7/SP6 ). PGEM-3Zf(-) (3.2kb, T7/SP6 ). Bluescript M13- (2.96kb, T7/T3 ). 按照试验的经济有效原则，本试验选用 Bluescript M13-。 B. 目的DNA序列与载体 连接 从所建立的cDNA文库中挑选出带有目的DNA片断的克隆，扩增后提取质粒，采用限制性内切酶处理或采用PCR特异扩增的方法分离出目的DNA片断;采用标准
