Recombinant Mouse GDF15 (N-8Hi

Recombinant Mouse GDF15 (N-8His-Flag) (C16R)

产品说明(Description)

 

Recombinant Mouse Growth Differentiation Factor 15 is produced by our Mammalian expression system and the target gene encoding Ser189-Ala303 is expressed with a 8His, Flag tag at the N-terminus.

Accession #: Q9Z0J7

Known as: Growth Differentiation Factor 15; Macrophage inhibitory cytokine 1; GDF-15; MIC-1; NAG-1; PLAB; PTGFB

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of 4mM HCl.

 

质量控制(Quality Control)

Purity:        Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin: Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. 

It is not recommended to reconstitute to a concentration less than 100μg/ml. 

Dissolve the lyophilized protein in 4mM HCl.

Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. 

Reconstituted protein solution can be stored at 4-7°C for 2-7 days.  

Aliquots of reconstituted samples are stable at < -20°C for 3 months.

 

背景(Background)

Growth Differentiation Factor 15 (GDF-15), also called Macrophage Inhibitory Cytokine 1 (MIC-1), is a divergent member of the TGF-beta superfamily. GDF15 can be secreted by a wide variety of cell types in response to a broad range of stressors. GDF-15 expression is dramatically upregulated during acute brain injury, cancer, cardiovascular disease, and inflammation, suggesting its potential value as a disease biomarker. GDF15 was shown to inhibit proliferation of primitive hematopoietic progenitors and introduced as a putative placental mediator of embryonic development. GDF15 has recently gained scientific and translational prominence with the discovery that its receptor is a GFRAL-RET heterodimer of which GFRAL is expressed solely in the hindbrain.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY