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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20
- 保质期：
2年
- 英文名：
SIR2L6
- 库存：
100
- 供应商：
上海烜雅生物科技有限公司
- 规格：
干粉/液体
基本信息
名称：pCDNA3-HA-p53人源基因质粒
别称: NM_001308090.1;HD7;HD7A;HDAC7A
启动子：CMV
复制子: pUC
原核抗性: Amp
筛选标记：G418
克隆菌株: Stbl3
培养条件: 37度
质粒属性
载体宿主：哺乳细胞
载体用途：蛋白表达
基因种属：人
基因类型：ORF
原核抗性：Amp
真核抗性：Neo/G418
荧光蛋白：
质粒简介
pCDNA3.0-HA-p53质粒由上海烜雅生物科技有限公司提供
质粒图谱
质粒序列
LOCUS Exported 9317 bp ds-DNA circular SYN 12-JUN-2017
FEATURES Location/Qualifiers
source 1..9317
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2028..2143
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
enhancer 2201..2504
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2505..2708
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 2815..3882
/codon_start=1
/label=SIRT6
/translation="MSVNYAAGLSPYADKGKCGLPEIFDPPEELERKVWELARLVWQSS
NVVFHTGAGISTASGIPDFRGPHGVWTMEERGLAPKFDTTFESARPTQTHMALVQLERV
GLLRFLVSQNVDGLHVRSGFPRDKLAELHGNMFVEECAKCKTQYVRDTVVGTMGLKATG
RLCTVAKARGLRACRGELRDTILDWEDSLPDRDLALADEASRNADLSITLGTSLQIRPS
GNLPLATKRRGGRLVIVNLQPTKHDRHADLRIHGYVDEVMTRLMKHLGLEIPAWDGPRV
LERALPPLPRPPTPKLEPKEESPTRINGSIPAGPKQEPCAQHNGSEPASPKRERPTSPA
PHRPPKRVKAKAVPS"
misc_feature 3891..4464
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 4478..5272
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPAT-CPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
misc_feature 5286..5874
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(5757..5768)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 6081..6714
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
primer_bind complement(6842..6858)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 6866..6882
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6890..6920)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6935..6956
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(7244..7832)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(8003..8863)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(8864..8968)
/gene="bla"
/label=AmpR promoter
polyA_signal 9016..9150
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatgtcg
2821 gtgaattacg cggcggggct gtcgccgtac gcggacaagg gcaagtgcgg cctcccggag
2881 atcttcgacc ccccggagga gctggagcgg aaggtgtggg aactggcgag gctggtctgg
2941 cagtcttcca atgtggtgtt ccacacgggt gccggcatca gcactgcctc tggcatcccc
3001 gacttcaggg gtccccacgg agtctggacc atggaggagc gaggtctggc ccccaagttc
3061 gacaccacct ttgagagcgc gcggcccacg cagacccaca tggcgctggt gcagctggag
3121 cgcgtgggcc tcctccgctt cctggtcagc cagaacgtgg acgggctcca tgtgcgctca
3181 ggcttcccca gggacaaact ggcagagctc cacgggaaca tgtttgtgga agaatgtgcc
3241 aagtgtaaga cgcagtacgt ccgagacaca gtcgtgggca ccatgggcct gaaggccacg
3301 ggccggctct gcaccgtggc taaggcaagg gggctgcgag cctgcagggg agagctgagg
3361 gacaccatcc tagactggga ggactccctg cccgaccggg acctggcact cgccgatgag
3421 gccagcagga acgccgacct gtccatcacg ctgggtacat cgctgcagat ccggcccagc
3481 gggaacctgc cgctggctac caagcgccgg ggaggccgcc tggtcatcgt caacctgcag
3541 cccaccaagc acgaccgcca tgctgacctc cgcatccatg gctacgttga cgaggtcatg
3601 acccggctca tgaagcacct ggggctggag atccccgcct gggacggccc ccgtgtgctg
3661 gagagggcgc tgccacccct gccccgcccg cccaccccca agctggagcc caaggaggaa
3721 tctcccaccc ggatcaacgg ctctatcccc gccggcccca agcaggagcc ctgcgcccag
3781 cacaacggct cagagcccgc cagccccaaa cgggagcggc ccaccagccc tgccccccac
3841 agacccccca aaagggtgaa ggccaaggcg gtccccagct gaggatcccg cccctctccc
3901 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
3961 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
4021 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
4081 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
4141 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
4201 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
4261 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
4321 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
4381 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
4441 ttcctttgaa aaacacgatg ataagcttgc cacaaccatg gctgaacaag atggattgca
4501 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
4561 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
4621 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
4681 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
4741 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
4801 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
4861 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
4921 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
4981 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
5041 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
5101 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
5161 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
5221 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaacgcgtct
5281 ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
5341 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
5401 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
5461 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
5521 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
5581 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
5641 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
5701 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
5761 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
5821 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
5881 ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
5941 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
6001 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
6061 tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct
6121 tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg
6181 gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc
6241 agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct
6301 gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc
6361 atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc
6421 ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg
6481 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc
6541 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
6601 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
6661 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
6721 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
6781 agaggccttg acattgctag cgtttaccgt cgacctctag ctagagcttg gcgtaatcat
6841 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
6901 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
6961 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
7021 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
7081 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
7141 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
7201 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
7261 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
7321 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
7381 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
7441 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
7501 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
7561 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
7621 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
7681 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
7741 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
7801 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
7861 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
7921 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
7981 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
8041 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
8101 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
8161 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
8221 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
8281 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
8341 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
8401 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
8461 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
8521 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
8581 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
8641 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
8701 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
8761 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
8821 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
8881 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
8941 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
9001 acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc
9061 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
9121 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
9181 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
9241 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
9301 actacaaact tagtagt
//
质粒菌株产品操作说明书
一、扩增流程
收到产品后，请先根据产品管壁标签来判断产品形式，并在扩增前准确查找该质粒菌株的抗性、感受态和培养温度。
1、质粒干粉（常温运输，存于-20度，90天保质期，请务必转化挑单克隆培养，不要直接使用和测序）
①收到质粒干粉后请先5000rpm离心1min，再加入20μl ddH2O去离子水溶解质粒；
②取1支100μl 感受态于冰上解冻10min，加入2μl质粒，再冰浴30min后，42℃热激60s，不要搅动，再冰浴2min；（从第二步开始均要在超净工作台中无菌操作）
③加入900μl无抗的LB液体培养基，180rpm震荡37℃培养45min；
④6000rpm离心5min，仅留100μl上清液重悬细菌沉淀，并涂布至目标质粒抗性的LB平板上；（可使用本平台的平板涂布专用玻璃珠进行涂布，可以比传统涂布方法获得更多转化子）
⑤将平板正向培养1h，再倒置37℃培养14h。如果要求是30度则培养20h；
（菌落过多则将质粒稀释后再转化。没有菌落则加入10μl质粒转化。另不要直接转表达感受态，要先转克隆感受态，重提质粒后再导入表达感受态）
⑥挑取单菌落至LB液体培养基中，加入对应抗生素，220rpm震荡培养14h，根据实验需要和质粒提取试剂盒说明书提取质粒。
2、甘油菌种（冰袋运输，存于-80℃，保质期90天，请务必划线挑单克隆培养）
四区划线后挑单菌落培养，酵母菌需要先液体复苏再四区划线，再挑单菌落液体培养。
3、穿刺菌种（冰袋运输，存于4℃，保质期7天）
穿刺接种，液体培养后四区划线，再挑单菌落液体培养。
4、菌落平板（冰袋运输，存于4℃，保质期7天）
直接挑取单菌落至液体培养基中。
5、液体质粒（冰袋运输，存于-20℃，保质期90天）
单独提取的液体质粒收到后可直接使用。
6、滤纸质粒（常温运输，存于-20度，90天保质期，请务必转化挑单克隆培养，不要直接使用和测序）
收到货后将滤纸画圈部分剪下放入EP管中，加100ul无菌水将滤纸浸湿并浸泡5min，吸取5ul质粒转化，离心全涂。
二、转化图片
名称：pCDNA3-HA-p53人源基因质粒
别称: NM_001308090.1;HD7;HD7A;HDAC7A
启动子：CMV
复制子: pUC
原核抗性: Amp
筛选标记：G418
克隆菌株: Stbl3
培养条件: 37度
质粒属性
载体宿主：哺乳细胞
载体用途：蛋白表达
基因种属：人
基因类型：ORF
原核抗性：Amp
真核抗性：Neo/G418
荧光蛋白：
质粒简介
pCDNA3.0-HA-p53质粒由上海烜雅生物科技有限公司提供
质粒图谱
质粒序列
LOCUS Exported 9317 bp ds-DNA circular SYN 12-JUN-2017
FEATURES Location/Qualifiers
source 1..9317
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2028..2143
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
enhancer 2201..2504
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2505..2708
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 2815..3882
/codon_start=1
/label=SIRT6
/translation="MSVNYAAGLSPYADKGKCGLPEIFDPPEELERKVWELARLVWQSS
NVVFHTGAGISTASGIPDFRGPHGVWTMEERGLAPKFDTTFESARPTQTHMALVQLERV
GLLRFLVSQNVDGLHVRSGFPRDKLAELHGNMFVEECAKCKTQYVRDTVVGTMGLKATG
RLCTVAKARGLRACRGELRDTILDWEDSLPDRDLALADEASRNADLSITLGTSLQIRPS
GNLPLATKRRGGRLVIVNLQPTKHDRHADLRIHGYVDEVMTRLMKHLGLEIPAWDGPRV
LERALPPLPRPPTPKLEPKEESPTRINGSIPAGPKQEPCAQHNGSEPASPKRERPTSPA
PHRPPKRVKAKAVPS"
misc_feature 3891..4464
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 4478..5272
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPAT-CPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
misc_feature 5286..5874
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(5757..5768)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 6081..6714
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
primer_bind complement(6842..6858)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 6866..6882
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6890..6920)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6935..6956
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(7244..7832)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(8003..8863)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(8864..8968)
/gene="bla"
/label=AmpR promoter
polyA_signal 9016..9150
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatgtcg
2821 gtgaattacg cggcggggct gtcgccgtac gcggacaagg gcaagtgcgg cctcccggag
2881 atcttcgacc ccccggagga gctggagcgg aaggtgtggg aactggcgag gctggtctgg
2941 cagtcttcca atgtggtgtt ccacacgggt gccggcatca gcactgcctc tggcatcccc
3001 gacttcaggg gtccccacgg agtctggacc atggaggagc gaggtctggc ccccaagttc
3061 gacaccacct ttgagagcgc gcggcccacg cagacccaca tggcgctggt gcagctggag
3121 cgcgtgggcc tcctccgctt cctggtcagc cagaacgtgg acgggctcca tgtgcgctca
3181 ggcttcccca gggacaaact ggcagagctc cacgggaaca tgtttgtgga agaatgtgcc
3241 aagtgtaaga cgcagtacgt ccgagacaca gtcgtgggca ccatgggcct gaaggccacg
3301 ggccggctct gcaccgtggc taaggcaagg gggctgcgag cctgcagggg agagctgagg
3361 gacaccatcc tagactggga ggactccctg cccgaccggg acctggcact cgccgatgag
3421 gccagcagga acgccgacct gtccatcacg ctgggtacat cgctgcagat ccggcccagc
3481 gggaacctgc cgctggctac caagcgccgg ggaggccgcc tggtcatcgt caacctgcag
3541 cccaccaagc acgaccgcca tgctgacctc cgcatccatg gctacgttga cgaggtcatg
3601 acccggctca tgaagcacct ggggctggag atccccgcct gggacggccc ccgtgtgctg
3661 gagagggcgc tgccacccct gccccgcccg cccaccccca agctggagcc caaggaggaa
3721 tctcccaccc ggatcaacgg ctctatcccc gccggcccca agcaggagcc ctgcgcccag
3781 cacaacggct cagagcccgc cagccccaaa cgggagcggc ccaccagccc tgccccccac
3841 agacccccca aaagggtgaa ggccaaggcg gtccccagct gaggatcccg cccctctccc
3901 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
3961 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
4021 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
4081 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
4141 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
4201 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
4261 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
4321 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
4381 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
4441 ttcctttgaa aaacacgatg ataagcttgc cacaaccatg gctgaacaag atggattgca
4501 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
4561 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
4621 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
4681 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
4741 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
4801 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
4861 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
4921 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
4981 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
5041 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
5101 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
5161 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
5221 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaacgcgtct
5281 ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
5341 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
5401 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
5461 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
5521 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
5581 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
5641 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
5701 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
5761 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
5821 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
5881 ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
5941 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
6001 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
6061 tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct
6121 tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg
6181 gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc
6241 agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct
6301 gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc
6361 atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc
6421 ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg
6481 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc
6541 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
6601 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
6661 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
6721 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
6781 agaggccttg acattgctag cgtttaccgt cgacctctag ctagagcttg gcgtaatcat
6841 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
6901 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
6961 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
7021 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
7081 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
7141 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
7201 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
7261 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
7321 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
7381 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
7441 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
7501 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
7561 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
7621 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
7681 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
7741 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
7801 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
7861 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
7921 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
7981 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
8041 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
8101 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
8161 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
8221 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
8281 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
8341 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
8401 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
8461 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
8521 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
8581 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
8641 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
8701 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
8761 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
8821 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
8881 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
8941 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
9001 acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc
9061 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
9121 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
9181 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
9241 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
9301 actacaaact tagtagt
//
质粒菌株产品操作说明书
一、扩增流程
收到产品后，请先根据产品管壁标签来判断产品形式，并在扩增前准确查找该质粒菌株的抗性、感受态和培养温度。
1、质粒干粉（常温运输，存于-20度，90天保质期，请务必转化挑单克隆培养，不要直接使用和测序）
①收到质粒干粉后请先5000rpm离心1min，再加入20μl ddH2O去离子水溶解质粒；
②取1支100μl 感受态于冰上解冻10min，加入2μl质粒，再冰浴30min后，42℃热激60s，不要搅动，再冰浴2min；（从第二步开始均要在超净工作台中无菌操作）
③加入900μl无抗的LB液体培养基，180rpm震荡37℃培养45min；
④6000rpm离心5min，仅留100μl上清液重悬细菌沉淀，并涂布至目标质粒抗性的LB平板上；（可使用本平台的平板涂布专用玻璃珠进行涂布，可以比传统涂布方法获得更多转化子）
⑤将平板正向培养1h，再倒置37℃培养14h。如果要求是30度则培养20h；
（菌落过多则将质粒稀释后再转化。没有菌落则加入10μl质粒转化。另不要直接转表达感受态，要先转克隆感受态，重提质粒后再导入表达感受态）
⑥挑取单菌落至LB液体培养基中，加入对应抗生素，220rpm震荡培养14h，根据实验需要和质粒提取试剂盒说明书提取质粒。
2、甘油菌种（冰袋运输，存于-80℃，保质期90天，请务必划线挑单克隆培养）
四区划线后挑单菌落培养，酵母菌需要先液体复苏再四区划线，再挑单菌落液体培养。
3、穿刺菌种（冰袋运输，存于4℃，保质期7天）
穿刺接种，液体培养后四区划线，再挑单菌落液体培养。
4、菌落平板（冰袋运输，存于4℃，保质期7天）
直接挑取单菌落至液体培养基中。
5、液体质粒（冰袋运输，存于-20℃，保质期90天）
单独提取的液体质粒收到后可直接使用。
6、滤纸质粒（常温运输，存于-20度，90天保质期，请务必转化挑单克隆培养，不要直接使用和测序）
收到货后将滤纸画圈部分剪下放入EP管中，加100ul无菌水将滤纸浸湿并浸泡5min，吸取5ul质粒转化，离心全涂。
二、转化图片
| P4824/pOTB7-MCL1人源基因质粒 |
| P4825/pOTB7-NDUFC2（1同义突变）人源基因质粒 |
| P3733/pMD18-TBK1人源基因质粒 |
| P4093/pMD18-STAT2人源基因质粒 |
| P3210/pUC18-YWHAE人源基因质粒 |
| P3734/pUC19-DDX58人源基因质粒 |
| P4335/pUC19-FEN1人源基因质粒 |
| P4094/pUC19-STAT1人源基因质粒 |
| P1423/pUC57-CNGA1-FLAG人源基因质粒 |
| P1632/pUC57s-GLO1人源基因质粒 |
| P1767/pUC57s-N6AMT1人源基因质粒 |
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