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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20℃
- 保质期:
3个月
- 英文名:
pCR-XL-TOPO-SHROOM2
- 库存:
50
- 供应商:
LSM BIO
- 规格:
2ug
pCR-XL-TOPO-SHROOM2
PVT33167 2ug
pCR-XL-TOPO-SHROOM2 Description
Alias:NM_001649.4;APXL; HSAPXLReplicator:pUC
Clone strain:DH5a
Culture conditions:37centigrade
Note:4851bp
Host:Escherichia coli
Use:Gene template
Species:Human
Gene type:cDNA
Prokaryotic resistance:Kan
Eukaryotic resistance:
Fluorescent protein:
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20ul ddH2O in to the tube of plasmid.
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文献和实验XL PCR Amplification of Long Targets from Genomic DNA
Long polymerase chain reaction (PCR)>(1 5 ), specifically, XL PCR (Extra-Long Polymerase Chain Reaction), has enabled amplification of expanded trinucleotide repeats of the neuromuscular disease myotonic dystrophy (6 ) a 9-kb HIV
Cloning vector to 1ml of the Taq-amplified PCR product2. Incubate for 5 minutes on your bench top3. Transform One Shot® Competent E. coli.Topo平端克隆方法和长PCR片断克隆法(Zero Blunt Topo PCR Cloning Kit)(Topo XL PCR Cloning Kit)因为越来越多高确限度热稳定酶如Pfx、Pfu被用于PCR扩增,使到PCR
PCR Cloning Kit)(Topo XL PCR Cloning Kit) 因为越来越多高确限度热稳定酶如Pfx、Pfu被用于PCR 扩增,使到PCR 后都只是平端产物。这种平端克隆 往往效率低,并且有非特异性背景菌落产生,造成筛选困难。 Topo平端克隆 主要改良载体设计,把ccdB (Control of cell death)基因并入到mcs中,如果没有DNA片断插入,ccdB基因则会表达
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