Ub⁺¹ polyclonal ant

Polyclonal and Monoclonal Antibodies

too yellow. Induce 2 more times , each time with 1 ug/ml LPS and collect sup after 2 days each. Pool the sups, filter and use as recomended. (Could be aliquoted and stored at -20 C.) Preparation of Media: for P3X63-Ag8.653: IMDM complete to 425 ml

Purification of acidic phosphatase from mustard seedlings

after 1 h by centrifugation in 50 ml centrifuge tube (approx. 35 ml per tube) at 31,000 x g ( estimate the rpm value using the rotor radius) for 30 min in the Hermle centrifuge at 4 °C (room B8220). 11. Decant the supernatant (SN2) into a grad

Purification of acidic phosphatase from mustard seedlings

. 10. Precipitate these proteins after 1 h by centrifugation in 50 ml centrifuge tube (approx. 35 ml per tube) at 31,000 x g (estimate the rpm value using the rotor radius) for 30 min in the Hermle centrifuge at 4 °C (room B8220). 11. Decant