- ￥1880
- 晶风生物
- TF13880R
- 中国
- 2025年07月06日
- Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- Rabbit
- Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
cGMP依赖性蛋白激酶2抗体
- 抗体英文名：
CGK2
- 靶点：
细胞浆 细胞膜 分泌型蛋白
- 浓度：
1mg/ml
- 应用范围：
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主：
Rabbit
- 适应物种：
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期：
一年
- 抗原来源：
Rabbit
- 目录编号：
TF13880R
- 级别：
I级
- 库存：
10
- 供应商：
晶风生物
- 标记物：
FITC/Alexa/CY357/BIo/HRP
- 克隆性：
Polyclonal
- 保存条件：
-20
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated synthetic peptide derived
- 规格：
50ul/100ul/200ul
产品规格：100ul/200ul(部分有50ul，如需更大包装或其他具体规格，请咨询客服)
研究领域：肿瘤 细胞生物 免疫学等
抗体来源：Rabbit
克隆类型：Polyclonal
交叉反应：Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用：WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状：Liquid
浓 度：1mg/ml
免 疫 原：KLH conjugated synthetic peptide derived
亚 型：IgG
纯化方法：affinity purified by Protein A
储 存 液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
CGK2cGMP依赖性蛋白激酶2抗体相关抗体示例（非本抗体，如需本抗体，请联系客服索要说明书）：
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.
paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.
CGK2cGMP依赖性蛋白激酶2抗体
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
欢迎新老客户咨询订购：CGK2cGMP依赖性蛋白激酶2抗体
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文献和实验举世闻名的「伟哥」，可能存在致命副作用？JAHA：促进这种疾病恶化，相关人群需谨慎使用
探讨了西地那非对实验性小鼠腹主动脉瘤（Abdominal Aortic Aneurysm, AAA）的影响，研究结果表明，西地那非可通过调节 SMC 收缩功能而加重了主动脉退变和腹主动脉瘤的进展。 图片来源：JAHA 研究背景 SMC 收缩主要依赖于肌球蛋白轻链（MLC）的磷酸化状态，受肌球蛋白轻链激酶（MLCK）和肌球蛋白轻链磷酸酶（MLCP）活性的调节。MLCK 激活导致平滑肌收缩，主要依赖于 Ca2+ 和钙调素的水平；相反，MLCP 的激活会导致平滑肌松弛。 cGMP 可以通过多
kinase，CDK）家族；丝裂原活化蛋白激酶（mitogen-activated protein kinase，MAPK）家族；糖原合成酶激酶 3（glycogen synthetase kinase 3，GSK3）；CDK 样激酶（CDK-like kinase，CLK）家族。 （5）蛋白激酶 G，即 cGMP 依赖性蛋白激酶（cGMP –dependent protein kinase，PKG），以 cGMP 为变构剂，在脑和平滑肌中含量较丰富。 （6）G 蛋白偶联受体激酶
Kinase,简称为cAPK),亦称为蛋白激酶A(Protein Kinase A, PKA)，目前后一种命名较为公认。cAMP可以作为该激酶的变构激活剂，使无活性的蛋白激酶A转变为有活性的蛋白激酶A(详见第十一章，图11－4)。活化了的蛋白激酶A可作用于多种与糖脂代谢相关的酶类、一些离子通道和某些转导因子，使它们发生磷酸化并改变其活性状态。 (2)蛋白激酶G 细胞内的另一种环核苷酸信使为环鸟苷酸－cGMP。cGMP作用于cGMP依赖性蛋白激酶－cGMP�dependent Protein
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