相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
细胞周期调控蛋白D1抗体
- 抗体英文名:
MSF/Cell division control protein septin D1
- 靶点:
细胞浆 细胞膜 分泌型蛋白
- 浓度:
1mg/ml
- 应用范围:
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主:
Rabbit
- 适应物种:
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期:
一年
- 抗原来源:
Rabbit
- 目录编号:
TF7755R
- 级别:
I级
- 库存:
10
- 供应商:
晶风生物
- 标记物:
FITC/Alexa/CY357/BIo/HRP
- 克隆性:
Polyclonal
- 保存条件:
-20
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived
- 规格:
50ul/100ul/200ul
产品规格:100ul/200ul(部分有50ul,如需更大包装或其他具体规格,请咨询客服)
研究领域:肿瘤 细胞生物 免疫学等
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用:WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状:Liquid
浓 度:1mg/ml
免 疫 原:KLH conjugated synthetic peptide derived
亚 型:IgG
纯化方法:affinity purified by Protein A
储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
MSF/CelldivisioncontrolproteinseptinD1细胞周期调控蛋白D1抗体相关抗体示例(非本抗体,如需本抗体,请联系客服索要说明书):
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.

paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.

MSF/CelldivisioncontrolproteinseptinD1细胞周期调控蛋白D1抗体
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
欢迎新老客户咨询订购:MSF/CelldivisioncontrolproteinseptinD1细胞周期调控蛋白D1抗体
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验51D的分子能够阻止DNA损伤。但这种分子同时也阻止了染色体保护端的自然消退,也就阻止了细胞逐步衰老死亡的过程。科学家使用免疫荧光标记检测的方法追踪癌细胞中的不同分子,结果发现,癌细胞染色体的端粒位置一直存在RD51D分子。而当科学家采用RNA干涉的方法屏蔽掉这种分子的作用之后,癌细胞受到严重损伤。多数癌细胞在7天内死亡,而使用“安慰剂”的对照组癌细胞则安然无恙。屏蔽RD51D分子功能还导致不同染色体在分子分裂过程中经常发生的融合现像消失。更重要的是,屏蔽RD51D分子功能之后,染色体中少于6千碱基
克隆的前癌基因,其转第活性已被证明。 c-myb 禽类成髓细胞增多症病毒辣-转化基因,c-myb的正常细胞性同源物,表达一种细胞核DNA结合蛋白质,被认为调节造血体系统的细胞增生和分化。 c-myc 一种前癌基因,在调节DNA合成、细胞凋亡、分化及细胞周期的进行中起重要作用。c-Myc蛋白质是一种短寿的细胞核磷酸蛋白质,通过联系转录机器和E-合盒无件在转录中起直接作用。c-Myc是一种大性螺旋-环-螺旋亮氨酸的拉链蛋白,相似于USFEY E2F蛋白质家族
的蛋白质衣壳。 Caspases:一个蛋白质酶家族,其成员在调亡(细胞程序性死亡)中起作用。 Catabolite repression(分解代谢物阻碍):由于葡萄糖增加引起一些细菌操纵子表达降低。是 cAMP 水平降低使CAP 调控蛋白质失活所导致。 cDNA:与RNA 互补的单链DNA,通过体内RNA 逆转录而合成。 cDNA clone (cDNA 克隆):代表一个RNA 的双链DNA 进入一个克隆载体。 Cell cycle (细胞周期):一次细胞分裂到另一次分裂
技术资料暂无技术资料 索取技术资料









