- ￥1880
- 晶风生物
- TF5015R
- 中国
- 2025年07月04日
- Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- Rabbit
- Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
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- 文献和实验
- 技术资料
- 抗体名：
酰基辅酶A脱氢酶长链抗体
- 抗体英文名：
ACADL
- 靶点：
细胞浆 细胞膜 分泌型蛋白
- 浓度：
1mg/ml
- 应用范围：
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主：
Rabbit
- 适应物种：
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期：
一年
- 抗原来源：
Rabbit
- 目录编号：
TF5015R
- 级别：
I级
- 库存：
10
- 供应商：
晶风生物
- 标记物：
FITC/Alexa/CY357/BIo/HRP
- 克隆性：
Polyclonal
- 保存条件：
-20
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated synthetic peptide derived
- 规格：
50ul/100ul/200ul
产品规格：100ul/200ul(部分有50ul，如需更大包装或其他具体规格，请咨询客服)
研究领域：肿瘤 细胞生物 免疫学等
抗体来源：Rabbit
克隆类型：Polyclonal
交叉反应：Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用：WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状：Liquid
浓 度：1mg/ml
免 疫 原：KLH conjugated synthetic peptide derived
亚 型：IgG
纯化方法：affinity purified by Protein A
储 存 液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
ACADL酰基辅酶A脱氢酶长链抗体相关抗体示例（非本抗体，如需本抗体，请联系客服索要说明书）：
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.
paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.
ACADL酰基辅酶A脱氢酶长链抗体
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
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文献和实验（Trans-y-enoyl CoA），脱下的两个氢原子由该酶的辅酶FAD接受生成FADH2。后者经电子传递链传递给氧而生成水，同时伴有1.5分子ATP的生成。 酰基CoA脱氢酶是该过程的关键酶，根据其催化的底物长度和类型，主要分为长链酰基辅酶A脱氢酶（LACD）、中链酰基辅酶A脱氢酶（MACD）和短链酰基辅酶A脱氢酶（SACD）。这类酶缺陷会导致严重的代谢紊乱，多为常染色体隐性遗传病，最常见的如酰基辅酶A缺乏症。 （2）加水： α、β烯脂酰CoA在烯酰CoA水合酶的催化下，加水生成β-羟
。这种"代谢-免疫-表观"的三维调控，使丙酮酰化成为连接Warburg效应与细胞命运的核心节点。 相关产品推荐 货号 名称 产品类别 S0B60315 S-RMabMix™ Pyruvoyl Lysine Rabbit mAb 丙酮酰化抗体 S0B0655 S-RMabMix™ Acetyllysine Rabbit mAb 乙酰化抗体 S0F0017 Premium Anti-acetyllysine agarose Beads 乙酰化琼脂糖珠 S0B0719 S
过氧化物酶、葡萄糖脱氢酶、谷氨酸转氨酶、乳酸脱氢酶、苹果酸脱氢酶和乙醇脱氢酶等）、DNA ／RNA、抗原／抗体、辅酶（NADH，NAD＋， Q0,Q10）、神经递质、维生素、体内代谢产物和其它生物小分子等多种重要生物活性物质及临床标识因子。具体内容如下： 1.电极材料及其表面功能化 研究生物物质的电子传递和界面行为并加以测定，自然会遇到生物物质电活性中心难以暴露、电子传输缓慢及电极污染、钝化等基本难题。要解决这些问题，必然涉及电极材料的选择和电极表面功能化。
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