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ampa receptor 3 抗体

  • 参考价格： ¥4125.0 - 5225.0
  • 品  牌：  abgent
  • 货  号：  AG1259
  • 适应物种：  人,小鼠,大鼠
  • 应用范围：  免疫印迹,免疫组化,免疫沉淀
  • 抗体类型：  一抗
  • 配制后对照抗原保存条件：  -20ºC.
  • 预吸附控制：  1 µg peptide per 1 µg antibody.
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  • 详细信息
  • 图片

详细信息

产品概览

背景： L-Glutamate, the major excitatory neurotransmitter in the central nervous system, operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein coupled receptors).The ligand-gated ion channel family consists of 15 members that have been subdivided into three families based on their pharmacological profile: the a-amino-3-hydroxy-5-methyl-4-isoazolepropionic acid (AMPA) preferring receptors, the N-methyl-D-aspartate (NMDA) preferring and the kainate preferring receptors. The AMPA receptor subfamily includes four members AMPA1 to AMPA4, also known as GluR1 to GluR4 respectively. The functional AMPA channel is believed to be a tetramer, with most neuronal AMPA receptors being heterotetramers composed of AMPA1 plus AMPA2 or AMPA2 plus AMPA3 channels, although homotetramers can also been found.AMPA receptors are permeable to cations Na+, K+and Ca2+.  The Ca2+permeability is dependent on the presence of AMPA2: whenever this subunit is present, the channel will be impermeable to Ca2+.1 Gating of AMPA receptors by glutamate is extremely fast and therefore the AMPA receptors mediate most excitatory (depolarizing) currents in the brain during basal neuronal activity. The depolarization caused by the activation of post-synaptic AMPA receptors is necessary for the activation of NMDA receptors that will open only in the presence of both glutamate and a depolarized membrane potential. Synaptic strength that is defined as the level of post-synaptic depolarization can be long term (hence the term long term potentiation, LTP) and therefore induce changes in signaling and protein synthesis in the activated neuron. These changes are associated with memory formation and learning. Changes in synaptic strength are thought to involve rapid movement of the AMPA receptors in and out of the synapses and a great deal of effort has focused in understanding the mechanisms that govern AMPA receptor trafficking.2 The exact physiological role of the AMPA3 receptor is not clear but a role in the modulation of oscillatory networks affecting sleep and breathing has been suggested.3 Alomone labs is pleased to offer a highly specific antibody directed against a well conserved epitope located in the extracellular N-terminal region of the rat AMPA Receptor 3. The antibody does not cross react with the related AMPA Receptor 2 subunit. Anti-AMPA Receptor 3 (GluA3) (extracellular) (#AG1259) antibody can be used in western blot, immunohistochemical, and immunoprecipitation applications and will recognize AMPA Receptor 3 from rat, mouse, human and dog samples.REFERENCESReferences 1. Dingledine, R.et al.(1999)Pharmacol. Rev.51, 7. 2. Lynch, M.A. (2004)Physiol. Rev.84, 87. 3. Steenland, H.W.et al.(2008)Eur. J. Neurosci.27, 1166.
其他名字： GLUA3

靶点信息

基因id： 29628

应用与保存

配制： 50 µl or 0.2 ml deionized water, depending on the sample size.
配制前保存条件： Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
配制前对照抗原保存条件： Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
配制后保存条件： The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
配制后抗体浓度： 0.6 mg/ml.
配制后缓冲液： Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
稀释浓度： WB~~1:400

其他

参考文献： References 1. Dingledine, R.et al.(1999)Pharmacol. Rev.51, 7. 2. Lynch, M.A. (2004)Physiol. Rev.84, 87. 3. Steenland, H.W.et al.(2008)Eur. J. Neurosci.27, 1166.
对照实验相关产品： Control peptide antigen (supplied with the antibody free of charge).
对照抗原配制： 100 µl water.
标记物： Affinity purified antibody, lyophilized powder
主要序列号： P19492
分子量： 100373 Da
同源性： Mouse, human, dog - identical.
验证肽： Confirmed by mass-spectrography and amino acid analysis.
规格： 0.2 ml 价格： ¥5225.00
规格： 50 µl 价格： ¥4125.00

  • Expression of AMPA Receptor 3 in mouse cerebellumImmunohistochemical staining of frozen perfusion-fixed free floating sections of mouse cerebellum using  Anti-AMPA Receptor 3 (GluA3) (extracellular)antibody (#AG1259). A. Distribution of AMPA Receptor 3 (red). B. Distribution of glial fibrillary acidic protein (green). C. Merge of the two images indicates that AMPA Receptor 3 is localized to Bergmann glia (vertical arrow) and to Purkinje cell soma (horizontal arrow). DAPI is used as the counterstain (blue).

  • Immunoprecipitation of rat cerebellum lysates:1. Cerebellum lysates.2. Cerebellum lysates +Anti-AMPA Receptor 3 (GluA3) (extracellular)antibody (#AG1259) + protein A  beads.3. Cerebellum lysates + pre-immune rabbit serum + protein A beads.Black arrow indicates the AMPA Receptor 3 (GluR3) while the red arrow shows the IgG heavy chain. Immunoblot was performed with Anti-AMPA Receptor 3 (GluA3) (extracellular) antibody (#AG1259).

  • Western blot analysis of rat cerebellum lysates:1.Anti-AMPA Receptor 3 (GluA3) (extracellular)antibody (#AG1259), (1:400).2. Anti-AMPA Receptor 3 (GluA3) (extracellular) antibody, preincubated with the control peptide antigen.

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