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nmda receptor 2b 抗体

  • 参考价格： ¥4125.0 - 5225.0
  • 品  牌：  abgent
  • 货  号：  AG1265
  • 适应物种：  人,小鼠,大鼠
  • 应用范围：  免疫印迹,免疫组化,免疫沉淀
  • 抗体类型：  一抗
  • 主要序列号：  Q00960
  • 分 子 量：  166071 Da
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  • 详细信息
  • 图片

详细信息

产品概览

背景： The NMDA receptors are members of the glutamate receptor family of ion channels that also include the AMPA and Kainate receptors. The NMDA receptors are encoded by seven genes: one NMDAR1 (or NR1) subunit, four NR2 (NR2A-NR2D) and two NR3 (NR3A-NR3B) subunits. The functional NMDA receptor appears to be a heterotetramer composed of two NMDAR1 and two NMDAR2 subunits. Whereas the NMDAR2 subunits that assemble with the NMDAR1 subunit can be either of the same kind (i.e. two NMDAR2A subunits) or different (one NMDAR2A with one NMDAR2B). NMDAR3 subunits can substitute the NMDAR2 subunits in their complex with the NMDAR1 subunit. The NMDAR is unique among ligand-gated ion channels in that it requires the simultaneous binding of two obligatory agonists: glycine and glutamate that bind to the NMDAR1 and NMDAR2 binding sites respectively. Another unique characteristic of the NMDA receptors is their dependence on membrane potential. At resting membrane potentials the channels are blocked by extracellular Mg2+. Neuronal depolarization relieves the Mg2+ blockage and allows ion influx into the cells. NMDA receptors are strongly selective for Ca2+ influx differing from the other glutamate receptor ion channels that are non-selective cation channels. Ca2+ entry through the NMDAR regulates numerous downstream signaling pathways including long term potentiation (a molecular model of memory) and synaptic plasticity that may underlie learning. In addition, the NMDA receptors have been implicated in a variety of neurological disorders including epilepsy, ischemic brain damage, Parkinson’s and Alzheimer’s disease. NMDA receptors expression and function are modulated by a variety of factors including receptor trafficking to the synapses and internalization as well as phosphorylation and interaction with other intracellular proteins.REFERENCESReferences 1. Dingledine, R.et al. (1999)Pharmacol. Rev. 51, 7. 2. Mayer, M.L. and Armstrong, N. (2004)Annu. Rev. Physiol. 66, 161. 3. Prybylowski, K. and Wenthold, R.J. (2004)J. Biol. Chem. 279, 9673. 4. Mayer, M.L. (2006)Nature440, 456.
其他名字： GLUN2B

靶点信息

基因id： 24410

应用与保存

配制： 50 µl or 0.2 ml deionized water, depending on the sample size.
配制前保存条件： Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
配制前对照抗原保存条件： Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
配制后保存条件： The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
配制后对照抗原保存条件： -20ºC.
配制后抗体浓度： 0.4 mg/ml.
配制后缓冲液： Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
稀释浓度： WB~~1:600IHC~~1:100

其他

参考文献： References 1. Dingledine, R.et al. (1999)Pharmacol. Rev. 51, 7. 2. Mayer, M.L. and Armstrong, N. (2004)Annu. Rev. Physiol. 66, 161. 3. Prybylowski, K. and Wenthold, R.J. (2004)J. Biol. Chem. 279, 9673. 4. Mayer, M.L. (2006)Nature440, 456.
对照实验相关产品： Control peptide antigen (supplied with the antibody free of charge).
对照抗原配制： 100 µl water.
标记物： Affinity purified antibody, lyophilized powder
同源性： Human, mouse - identical.
提取： Lyophilized powder. Reconstituted antibody comtains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
预吸附控制： 1 µg peptide per 1 µg antibody.
验证肽： Confirmed by amino acid analysis.
规格： 0.2 ml 价格： ¥5225.00
规格： 50 µl 价格： ¥4125.00

  • Expression of NMDA Receptor 2B in rat cortexImmunohistochemical staining of rat parietal cortex frozen sections stained withAnti-NMDA Receptor 2B (GluN2B) (extracellular)antibody (#AG1265), (1:100). A. NMDA Receptor 2B (green) appears in the pyramidal layer of layer 5. B. Staining of neurofilament 200 (red) identifies neuronal processes. C. Confocal merge demonstrates localization of NMDA Receptor 2B in cells.

  • Expression of NMDA Receptor 2B in rat hippocampusImmunohistochemical staining of rat hippocampal CA1 frozen sections stained withAnti-NMDA Receptor 2B (GluN2B) (extracellular)antibody (#AG1265), (1:100). A.  NMDA Receptor 2B (green) appears in the pyramidal layer of CA1. B.  Staining of neurofilament 200 (red) identifies neuronal processes. C. Confocal merge demonstrates localization of NMDA Receptor 2B in cells and not in processes.

  • Immunoprecipitation of rat brain lysates:1. Cell lysates.2. Cell lysates + protein A beads +Anti-NMDA Receptor 2B (GluN2B) (extracellular)antibody (#AG1265). 3. Cell lysates + protein A beads + pre-immune rabbit serum.  Red arrow indicates the NMDA Receptor 2B protein while the black arrow shows the IgG heavy chain. Immunoblot was performed with Anti-NMDA Receptor 2B (GluN2B) (extracellular) antibody.

  • Western blot analysis of rat brain lysates:1.Anti-NMDA Receptor 2B (GluN2B) (extracellular)antibody (#AG1265), (1:600).2. Anti-NMDA Receptor 2B (GluN2B) (extracellular) antibody, preincubated with the control peptide antigen.

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