| 背景： | In order to function in optimal conditions, cells must maintain a close to neutral intracellular pH. They have adopted various mechanisms in order to do so, one of which is via Na+/H+exchangers (NHEs). Genes belonging to this group are expressed along a very broad range of organisms and are essential for protecting cells against intracellular acidification1. To date, nine genes have been identified in mammals; NHE1-9. These membrane proteins have 10-12 transmembrane domains depending on whether a splice variant is expressed and an intracellular N-terminal. The C-terminal domain can be either intracellular or extracellular, also depending whether a splice variant of the protein is involved. The C-terminal part of the protein also undergoes posttranslational modification such as phosphorylation2. Both NHE-1 and NHE-2 have an extracellular loop which is glycosylated1,3,4. Under physiological conditions, the Na+/H+exchanger mediates the exchange of one extracellular Na+ion for one intracellular proton, thereby keeping the overall charge neutral1. The extracellular binding site of Na+is not selective as it can also bind Li+and H+ 1,5,6. K+ions inhibit NHE-1 but have no effect on NHE-27. The activation of NHE-1 and NHE-2 is sensitive to intracellular acidic pH. Under physiological conditions, both exchangers are not active and upon a drop of intracellular pH, they are rapidly activated1,5,8. NHE-1 expression is ubiquitous and may serve as a housekeeping gene9. Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat NHE-1. Anti-Na+/H+Exchanger 1 (NHE-1) antibody (#AG1160) can be used in western blot immunocytochemistry and indirect flow cytometry applications. It has been designed to recognize NHE-1 from human, rat and mouse samples.REFERENCESReferences 1. Counillon, L. and Pouyssegur, J. (2000)J. Biol. Chem.275, 1. 2. Kemp, G.et al.(2008)Channels2, 329. 3. Counillon, L.et al.(1994)Biochemistry33, 10463. 4. Tse, C.et al.(1994)Biochemistry33, 12954. 5. Paris, S. and Poouyssegur, J. (1983)J. Biol. Chem.258, 3503. 6. Aronson, P.S.et al.(1983)J. Biol. Chem.258, 6767. 7. Yu, F.H.et al.(1993)J. Biol. Chem.268, 25536. 8. Aronson, P.S.et al.(1982)Nature299, 161. 9. Orlowski, J. and Grinstein, S. (1997)J. Biol. Chem.272, 22373. |
