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nectin-1 抗体
- 参考价格： ¥3025.0 - 5225.0
- 品 牌： abgent
- 货 号： AG1418
- 适应物种： 人,小鼠,大鼠
- 应用范围： 免疫印迹,免疫组化,流式细胞技术,免疫细胞化学
- 抗体类型： 一抗
- 对照抗原配制： 100 µl DDW.
- 配制后对照抗原保存条件： -20ºC.
产品概览
| 背景： | Nectins, which were originally identified as virus receptors, are members of the cell-cell adhesion molecule (CAM) family. They are Ca2+-independent immunoglobulin-like CAMs. The nectin family comprises four members, nectin-1, nectin-2, nectin-3 and nectin-4, which are encoded by the PVRL1, PVRL2, PVRL3 and PVRL4 genes, respectively1. In the central nervous system, these cell adhesion molecules aggregate in formations, termed puncta adherentia junctions, which are mechanical adhesive sites that connect pre- and postsynaptic membranes2. All nectins contain an extracellular region with three immunoglobulin-like loops (one V type and two C2 types), a single membrane-spanning region and a cytoplasmic tail. Nectins directly bind afadin, an F-actin-binding protein, through their cytoplasmic tails1. Nectin-1 is expressed at cell-cell junctions in human and mouse epidermis. In mice lacking the gene encoding nectin-1 (Pvrl1−/−mice), the expression of loricrin, a differentiation marker and a major component of cornified cell envelopes in the epidermis, is downregulated and newborn pups have a shiny and slightly reddish skin3. Mutations in human PVRL1 are implicated in cleft lip or palate-ectodermal dysplasia syndromes, which includes Zlotogora–Ogur syndrome and Margarita Island ectodermal dysplasia4. Both of these are autosomal recessive disorders that are clinically characterized by unusual facial appearance, dental anomalies, hypotrichosis, palmoplantar hyperkeratosis and onychodysplasia, syndactyly, cleft lip or palate, and in some cases, mental retardation5. REFERENCESReferences 1. Rikitake, Y.et al.(2012)J. Cell Sci.125, 3713. 2. Fantin, M.et al. (2013)PLoS One8, e56897. 3. Wakamatsu, K.et al. (2007)J. Biol. Chem. 282, 1817. 4. Sözen, M.A.et al. (2001)Nat. Genet.29, 141. 5. Suzuki, K.et al. (2000)Nat. Genet.25, 427. |
| 其他名字： | Nectin-1 |
靶点信息
| 基因id： | 5818 |
应用与保存
| 配制： | 25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size. |
| 配制前保存条件： | Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C. |
| 配制前对照抗原保存条件： | Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C. |
| 配制后保存条件： | The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min). |
| 配制后抗体浓度： | 0.8 mg/ml. |
| 稀释浓度： | WB~~1:200IHC~~1:100ICC~~1:100FC~~1:15 |
其他
| 参考文献： | References 1. Rikitake, Y.et al.(2012)J. Cell Sci.125, 3713. 2. Fantin, M.et al. (2013)PLoS One8, e56897. 3. Wakamatsu, K.et al. (2007)J. Biol. Chem. 282, 1817. 4. Sözen, M.A.et al. (2001)Nat. Genet.29, 141. 5. Suzuki, K.et al. (2000)Nat. Genet.25, 427. |
| 对照实验相关产品： | Control peptide antigen (supplied with the antibody free of charge). |
| 标记物： | Affinity purified antibody, lyophilized powder |
| 主要序列号： | Q15223 |
| 分子量： | 57158 Da |
| 同源性： | Rat - identical; mouse - 13/14 amino acid residues identical. |
| 提取： | Lyophilized powder. Resuspended antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3. |
| 预吸附控制： | 1 µg peptide per 1 µg antibody. |
| 验证肽： | Confirmed by mass-spectrography and amino acid analysis. |
| 规格： | 0.2 ml | 价格： | ¥5225.00 |
| 规格： | 50 µl | 价格： | ¥4125.00 |
| 规格： | 25 µl | 价格： | ¥3025.00 |
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