- 移动端
angiotensin ii receptor type-1 抗体
- 参考价格： ¥3025.0 - 5225.0
- 品 牌： abgent
- 货 号： AG1463
- 适应物种： 人,小鼠,大鼠
- 应用范围： 免疫印迹,免疫组化,流式细胞技术,免疫细胞化学
- 抗体类型： 一抗
- 基因id： 185
- 配制后对照抗原保存条件： -20ºC.
产品概览
| 背景： | Angiotensin II receptor type 1 or AT1 receptor is one of the receptors that binds the octapeptide hormone Angiotensin II (Ang II). Ang II is the peptide hormone that generates most of the known effects of the renin-angiotensin system (RAS). Ang II is generated from the angiotensinogen protein by the actions of renin, angiotensin converting enzyme (ACE) and other peptidases. Ang II has a central role in cardiovascular homeostasis by regulating vasoconstriction, renal Na+ and water readsorption. In addition, Ang II induces cell growth and proliferation and has pro-inflammatory effects. Most of the physiological actions of Ang II are mediated by AT1 receptor a member of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.The AT1 receptor is coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (InsP3) and intracellular Ca2+ mobilization. In addition to the rapid actions mediated by InsP3 and Ca2+ signaling, AT1 receptor elicits other signaling responses including activation of the MAPK and Jak/STAT. Together, these signaling pathways mediate most of Ang II cellular responses that include, control of hormone secretion, regulation of cell growth and apoptosis, regulation of ion channels activation and cell migration. In accordance with the pleiotropic actions of Ang II, the AT1 receptor has a wide distribution with high levels detected in the adrenal gland, kidney, brain, heart, liver, etc. As is the case with many other peptide receptors, the AT1 receptor undergoes ligand-induced endocytosis, a process that contributes to receptor desensitization and sequestration and contributes to regulate receptor signaling.REFERENCES1. Sasaki, K. et al. (1991) Nature 351, 230.2. Murphy, T.J. et al. (1991) Nature 351, 233.3. De Gasparo, M. et al. (2000) Pharmacol. Rev. 52, 415.4. Hunyady, L. and Catt, K.J. (2006) Mol. Endocrinol. 20, 953. |
| 其他名字： | AGTR1 |
应用与保存
| 配制： | 25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size. |
| 配制前保存条件： | Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C. |
| 配制前对照抗原保存条件： | Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C. |
| 配制后保存条件： | The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min). |
| 配制后抗体浓度： | 0.7 mg/ml. |
| 应用详情： | Western blot analysis (WB):- Rat heart lysate (1:250) (see Wang, H. et al. (2013) in Product Citations).- Mouse lung lysate (1:1000) (see Rodriguez-Machado, M.G. et al. (2013) in Product Citations).- Sheep brain medulla lysate (1:200) (see Marshall, A.C. et al. (2013) in Product Citations). Indirect flow cytometry (IFC):- Mouse gastrocnemius muscle cells (1:100) (see Yoshida, T. et al. (2013) in Product Citations).Immunocytochemistry (IC):- Mouse isolated myofibers (1:100) (see Yoshida, T. et al. (2013) in Product Citations). |
| 稀释浓度： | WB~~1:200ICC~~1:100 |
其他
| 参考文献： | 1. Sasaki, K. et al. (1991) Nature 351, 230.2. Murphy, T.J. et al. (1991) Nature 351, 233.3. De Gasparo, M. et al. (2000) Pharmacol. Rev. 52, 415.4. Hunyady, L. and Catt, K.J. (2006) Mol. Endocrinol. 20, 953. |
| 对照实验相关产品： | Control peptide antigen (supplied with the antibody free of charge). |
| 对照抗原配制： | 100 µl water. |
| 标记物： | Affinity purified antibody, lyophilized powder |
| 主要序列号： | P30556 |
| 分子量： | 41061 Da |
| 同源性： | Mouse, rat, rabbit, dog - identical. |
| 提取： | Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3. |
| 预吸附控制： | 1 µg peptide per 1 µg antibody. |
| 验证肽： | Confirmed by amino acid analysis. |
| 规格： | 50 µl | 价格： | ¥4125.00 |
| 规格： | 0.2 ml | 价格： | ¥5225.00 |
| 规格： | 25 µl | 价格： | ¥3025.00 |
- 相关信息
| 产品名 | 品牌名 |
|---|---|
| vpac1 抗体 | abgent |
| trka 抗体 | abgent |
| p2x1 receptor 抗体 | abgent |
| nectin-1 抗体 | abgent |
| mglur7 抗体 | abgent |