自噬抑制剂CQ或者3-MA干预条件

我做高糖，如果用CQ或者3-MA干预，是CQ和高糖一起培养细胞24h（假设是24h），还是先高糖培养XX时间，再CQ/3-MA干预24h，还是先CQ/3-MA干预24h，再高糖培养XX时间。

比如有一篇文献里面说：To evaluate the effect of high-glucose conditions on autophagy,

HK-2 cells were incubated for up to 72 h in DMEM contain­ing either low levels of glucose (5 mM, LG), high levels of glucose (30 mM, HG) or CQ (40 μM) with/without parical­citol (0.5 ng/ml) treatment

上面这个文献说的是CQ和高糖一起培养吗