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1 开机 1.1 电源线插入仪器电源插座，仪器必须有良好接地！ 1.2 按电源开关，接通电源，预热30分钟后，进行校准。 2 校准 仪器使用前必须进行校准！ 将“选择”开关量程 选择开关旋钮指向“检查”，“常数”补偿调节旋钮指向“1”刻度线，“温度”补偿调节旋钮指向&ldquo ...

一、仪器特点 1、仪器采用高性能集成电路组成性能稳定的正弦波发生器，因此显示稳定、漂移小； 2、仪器采用了相敏检波器，抑制了由电极引线分布电容对测量的影响，因而本仪器不用电容补偿调节器，换档自动补偿并能测量低电导值； 3、仪器信号源频率和量程同步切换，使用方便； 4、仪器可在全量程范围内直读电导，只需校正一次电极常数，使用极为方便； 5、仪器内装有热反馈电路和驱湿元件，仪器使用不受温度和湿度影响； ...

Passaging cells Pour out media from flasks. Wash with Hanks. 5 ml per flask. Tilt around then dump. Add 4 ml of Trypsin / EDTA to each flask. Tip then bang. Add 20% FBS NCTC media and tilt. 4 ml p ...

基因突变(gene mutation)是遗传病和肿瘤发生的根本原因，检测与遗传病及恶 性肿瘤发生有关的突变基因(mutant gene)是分子生物学，医学遗传学及肿瘤学研究 的热点，它对阐明遗传病和肿瘤发生的分子生物学基础及其诊断和早期诊断具有重要 ＼意义，分子生物学技术的发展，尤其是PCR技术的出现及近年来以PCR技术为基础， 结合传统技术的突变基因分析方法为人们提供了许多快速、简便、准确的基因 ...

荧光定量PCR技术具有简便、灵敏、准确等优点，目前已经在乙肝和性病的诊断和治疗中得到了广泛的应用，但在肿瘤方面的应用还处在研究和开发阶段。本文综述近年国内外相关荧光定量PCR技术在肿瘤研究中的应用

由Mullis等在1983年建立的PCR技术已成为分子生物学、遗传学等学科研究领域的经典实验方法。近年来该技术本身获得了长足发展可靠性不断提高在聚合酶链式反应基本原理的基础上发展了一系列新的概念和实验方法在生命科学研究中具有重要价值。 实时定量RT - PCR的原理、方法和应用 1、实时定量RT - PCR的原理 实时定量PCR的发明归功于两个重要的发现:一是发现DNA Taq酶有5′ ...

PCR技术基本原理 类似于DNA的天然复制过程，其特异性依赖于与靶序列两端互补的寡核苷酸引物。PCR由变性--退火--延伸三个基本反应步骤构成： 1、模板DNA的变性：模板DNA经加热至93℃左右一定时间后，使模板DNA双链或经PCR扩增形成的双链DNA解离，使之成为单链，以便它与引物结合，为下轮反应作准备； 2、模板DNA与引物的退火(复性)：模板DNA经加热变性成单链后，温度降至55℃左右，引 ...

FocusonPCR IntroductionMore than 15 years after its initial description the polymerase chain reaction (PCR) has become a standard molecularbiology tool and is the most widely used method o ...

DNA Marker产品选择指南 1.Marker选择标准（1）应选择在目标片段大小附近ladder较密的marker，这样对目标片段大小的估计较准确。（2）所选marker应能清楚反映目标片段的大小，且次要片段大小也能反映出来。如作酶切鉴定时，目的片段和切后载体片段最好能在同一个marker中反映出来，若二者不能兼顾，将前者作为主要考虑要素。 2.常见问题分析Q：为什么marker条带非常模糊， ...

Disruption by Fusion PCR 1) In separate PCR reactions amplify the 5' and 3' ends of the gene of interest with primers about 200 bases apart. Primer 2 should begin with 24 nts complementary to t ...

Terminal deoxynucleotidyl Transferase-mediated dUTP nick end labeling (TUNEL) is an in situ method for detecting the 3'-OH ends of DNA exposed during the internucleosomal cleavage that occurs during a ...

Detection of Alu by PCR In this experiment polymerase chain reaction (PCR) is used to amplify a nucleotide sequence from chromosome 8 to look for an insertion of a short DNA sequence called Alu ...

Protocol for Paraffin Sections: Dewax paraffin sections: Incubate slides 55°C 30 min. Xylenes 2 times 2 min. each 100% EtOH 2 times 2 min. each&nb ...

Protocol I: Triton X-100 Lysis Buffer In 96 flat-wells plate incubate 4x10 6 target cells (40 wells of 105 per well) with desired concentration of effectors (105 target cells per well). After incubat ...

Designing PCR programs 1Basic Principles The requirement of an optimal PCR reaction is to amplify a specific locus without any unspecific by-products. Therefore annealing needs to take place at a suff ...

Materials from a workshop held March 1997 in Modena Italy. All materials reproduced with permission. Introduction A. Cossarizza Workshop Sponsors Methods in analysis of apoptosis and cell necrosis. Z. ...

BrdU Incorporation Protocol Day 1 Label cells with media containing100 μM BrdU (6 μL/mL of 5 mg/mL stock): Diploid Fibroblasts 4-6 hours ES cells 30 minutes 3T3 cells 2.5 hours Trypsinize ce ...

immunofluorescence labeling; that is the antibodies have the fluorescent dye attached. Direct labeling is simpler and quicker than indirect labeling. We strongly recommend direct labeling if you're pl ...

FIXATION and DNA Staining for Cell Cycle Analysis BackgroundThis method of DNA staining utilizes ethanol to fix the cells and permeabilize the membrane which allows the dye (Propidium Iodide) to enter ...

A method to re-PCR unique bands from products of mixed size INTRODUCTION The products of a PCR reaction - especially when this is done on eukaryotic genomic DNA and when using degenerate ...