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Protocol for extracting DNA from ES Cells starting from the 96-well plate but processing in an eppendorf tube to recover more of the DNA . NOTE- THIS TAKES A LOT OF TIME if you do the whole plate this ...

Materials: 10X restriction enzyme buffer (see manufacturer's recommendation) DNA sterile water restriction enzyme phenol:chloroform (1:1) 1.Add the following to a microfuge tube: 2 μl of appropria ...

CAT ASSAY 1. Transfer cells to a 15 ml tube. 2. Add 5 ml TBS- to flasks shake & pour into tubes. 3. Spin down the cells @ 1k rpm for 5'. 4. Resuspend in 1 ml TBS- . 5. Transfer to 1.5 ml tube ...

Prepare a ligation mix: Ligation Mix (2x) ...

Purification of DNA from agarose gels is an essential method involved in the sub-cloning of DNA fragments. The following method describes a variation of the method of Vogelstein and Gillespie ...

Subcloning should be easy and fast and work every time. The following protocols minimize the number of manipulations required to prepare DNA fragments for ligations thereby both saving time and increa ...

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Can anyone recommend a good kit for genome walking? I would like to find out the promoter sequence of a known gene. Is genome walking the way to do it? thanks a million for any suggestions! -kiwi- Kiw ...

Purpose: Purification of oligonucleotides (which have already been purified by reverse phase) can increase the efficiency of site directed mutagenesis from around 30% to ~75% or greater in some cases ...

Prepare a 100 µl reaction mixture containing the DNA of interest in the appropriate 1X restriction enzyme buffer. Divide the reaction mixture between five prechilled microcentrifuge tubes such t ...

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下表中列出了最适温度不是37°C的限制性内切酶在37°C条件下的酶活性。 酶 最适温度 37℃%活性 ...

Molecularpourous membrane tubing is used for desalting protein and DNA isolation / purification. It comes in several diameters and pore sizes (for molecular weight conversions for nucleic acids see ge ...

Used to removed unincorporated nucleotides from labelling reactions. Prepare Sephadex G-50 (medium) by adding appropriate amount of dry beads to 100 ml TE buffer such that the beads will swell to 50 m ...

(adapted from Bruce A. Roe Department of Chemistry and Biochemistry The University of Oklahoma Norman Oklahoma 73019 broe@ou.edu) A. Sonication The generation of DNA fragments by sonication is perform ...

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Terminal Deoxynucleotidyl Transferase (TdT) is an enzyme that catalyzes the repetitive addition of mononucleotides from dNTPs to the terminal 3´-OH of a DNA initiator accompanied b ...

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Material/reagents: • 2X binding buffer: 20 mM Tris pH 7.5 100 mM NaCl 2 mM EDTA ...