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This is a scaled up version of the Baron protocol which has been modified to achieve purity comparable to CsCl preps. I have not sorted out strain variations yet but HB101 works well. 1、Solutions Sol ...

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琼脂糖凝胶层析的技术数据 琼脂糖凝胶作为核酸检测主要也是理想材料，使用十分广泛，其理化性质一些参数如下表： （琼脂糖是琼脂内非离子型的组分，它在 0 ~ 4 ℃， pH 4~9 范围内是稳定的。） 名称、型号 ...

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10X TBE, per liter Sequencing dye ------------------- ---------------- 108g Tris base 80% formamide 55g boric acid 10mM NaOH 40ml 0.5M EDTA 1mM EDTA 0.1% xylene cyanol 10% APS 0.1% bromophenol blue ------------------- dissolve 0.1g ammonium persulfate in 10ml dH2O and store frozenGEL COMPOSITIONS per liter 6% 8% 20% ------------------------------------------------ acrylamide 57g 76g 190g bis acrylamide 3g 4g 10g urea 500g 500g 500g 10X TBE 100ml 100ml 100ml ddH2O 300ml 300ml 300ml

Phenol (removes protein) 1、add equal volume of Phenol (= tris-saturated Phenol-Chloroform-Isoamyethanol)2、vortex3、spin 2 minutes at 12000 rpm 4°C4、transfer supernatant to a fresh tube (avoi ...

1. Add an equal volume (equal to sample volume) of P/C to sample. 2. Mix (shake don't vortex). 3. Take aqueous (upper) layer. (If dirty sample repeat Ph/Chl step until interface is fairly clean). 4. A ...

1. For double-stranded DNA templates: a. Denature template: 10ml DNA (5-10g or alkaline lysis mini prepDNA) 8ml ddH2O　　2ml 2N NaOH　　-incubate 30' at 37℃. b. Dry-ice precipitate: +10ml 4M NH4OAc 100ml ...

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This procedure is used to analyze inserts in pUC-derived plasimids. 1. Suspend E. coli colonies harbouring plasmids in 50 microliters of TE. 2. Incubate for 5 min at 95 degrees or in boiling water. 3. ...

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1) Prior to any ligation reaction you should always run one gel in which purified insert and cut backbone are run side by side preferably beside a known amount of cut DNA.You can then use this t ...

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