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This a brief protocol for plasmid mini preparation.

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Immediately before indcution it is advisable to test the culture to determine the fraction of cells that still carry the target plasmid. This in ...

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Media Need LB plates (9 cm or 14 cm)to plate the library and do secondary screens.I typically screen about 500000-1 million plaques for a primary cDNA screen.This fits on 11-22 14 cm plates.I do the s ...

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Restriction enzymes are expensive ($40 to $200 a vial): keep the enzymes at -20℃. Plan your digests to be small and convenient. The digest is composed of DNA sample (volume should not be more than abo ...

This protocol gives M13 quality sequence when used with the Quick DNA Plasmid Prep. protocol D.2.1、Solutions Denaturation Solution　　2 M NaOH 200 ml 10N NaOH　　2 mM EDTA 4 ml 0.5 M EDTA pH 8.0　　 ...

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基因工程：有目的的通过分子克隆技术，人为的操作改造基因，改变生物遗传性状的系列过程。 载体：能在连接酶的作用下和外源DNA片段连接并运送DNA分子进入受体细胞的DNA分子。 转化：指质粒DNA或以它为载体构建的重组DNA导入细菌的过程。 感染：以噬菌体、粘性质粒和真核细胞病毒为载体的重组DNA分子，在体外经过包装成具有感染能力的病毒或噬菌体颗粒，才能感染适当的细胞，并在细胞内扩增。 转导：指以噬菌 ...

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