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下面的简单方案是Clhen等（1972）所用方法的变通方案，常用于成批制备感受态细菌，这些细菌可使每微克螺旋质粒ＤＮＡ产生5x106-2x107个转化菌落，这样的转化效率足以满足所有在质粒中进行的常规克隆 的需要。该方法完全适用于大多数大肠杆菌菌株，并且比方案Ｉ快速，重复性更好。该法制备的感受态细胞 可贮存于-70℃，但保存时间过长会使转化效率在一定程度上受到影响。

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Probes should be stored frozen both when lyopholized or in solution. Aliquoting your probe into additional vials after resuspension can help to minimize potential breakdown due to ...

This a brief protocol for plasmid mini preparation.

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Immediately before indcution it is advisable to test the culture to determine the fraction of cells that still carry the target plasmid. This in ...

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Media Need LB plates (9 cm or 14 cm)to plate the library and do secondary screens.I typically screen about 500000-1 million plaques for a primary cDNA screen.This fits on 11-22 14 cm plates.I do the s ...

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Restriction enzymes are expensive ($40 to $200 a vial): keep the enzymes at -20℃. Plan your digests to be small and convenient. The digest is composed of DNA sample (volume should not be more than abo ...

This protocol gives M13 quality sequence when used with the Quick DNA Plasmid Prep. protocol D.2.1、Solutions Denaturation Solution　　2 M NaOH 200 ml 10N NaOH　　2 mM EDTA 4 ml 0.5 M EDTA pH 8.0　　 ...