全部

Secondary structure of prion mRNA Luck R; Steger G; Riesner D Institut fur Physikalische Biologie Heinrich-Heine-Universitat Dusseldorf FRG. J Mol Biol 258: 813-26 (1996) An algorithm for prediction ...

All solutions used in RNA preparation should be treated with DEPC as follows: add DEPC as follows: add DEPC to 0.1% and vigorously stir for one hour to O/N followed by autoclaving for 30 minutes ...

RNAi相关文章（for free） Prospects of RNA interference therapy for cancer S IPai Y-YLin BMacaes AMeneshian C-FHung and T-CWu Gene Ther 13: 464-477; ...

(1) Core out a single well separated plaque from the agar plate using a 200µl pipette tip and place it into 500µl of SM buffer in a 1.5ml microtube: SM buffer 5.8g of NaCl 2.0g of M ...

Using siRNA for gene silencing is a rapidly evolving tool in molecular biology. There are several methods for preparing siRNA such as chemical synthesis in vitro transcription siRNA expression vectors ...

We use the Promega Ribomax Large Scale RNA Production System T7 (Cat. No.: P1300) to produce dsRNAs and the RNAi Exp.html" target=_blank> Jack Dixon protocol ( RNAi _Dixon.html" target=_b ...

In the past poly(A)+ RNA has not been detected in prokaryotes. In the early 80s a new method of isolating RNA from bacteria was developed involving lysis by protease K in the presence of SDS and ...

Background Information of RNA i RNA interference (RNA i) is a biological process in which the introduction of double-stranded RNA (dsRNA ) into a cell results in targeted post-tranional gene silencing ...

RNA for S1 or PE analysis must be free of chromosomal DNA. This can be accomplished by extraction of the RNA with hot phenol but phenol can especially if its pH is acidic cause the specific loss ...

alimama_pid="mm_10043573_137377_1822161"; alimama_titlecolor="FF9900"; alimama_descolor ="552c55"; alimama_bgcolor="f7f7f7"; alimama_bordercolor="f7f ...

A modification of the basic immunofluorescence staining and flow cytometric analysis protocol can be used for the simultaneous analysis of surface molecules and intracellular cytokines at single-cell ...

1. Snap freeze ~107 cells or ~100 mg of tissue in liquid nitrogen or dry ice/ethanol. 2. Transfer the frozen sample to a mortar and pestle and grind to a fine powder. Or use a hand-held tissue grinder ...

In vitro transcription with yeast nuclear extract Steve Hahn Last Modified Fri Apr 25 2003 Wear gloves throughout use RNAse free solutions (either autoclaved or sterile filtered) and clean bench and ...

siRNA Design RNAi target selection rules : Targeted regions on the cDNA sequence of a targeted gene should be located 50-100 nt downstream of the start codon (ATG). Search for sequence motif AA ...

alimama_pid="mm_10043573_137377_1822161"; alimama_titlecolor="FF9900"; alimama_descolor ="552c55"; alimama_bgcolor="f7f7f7"; alimama_bor ...

000" height="300" width="500" classid="clsid:D27CDB6E-AE6D-11cf-96B8-444553540000" How does RNAi work? Genetic and biochemical data indicate a possible two-step mechanism for RNA i ...

Reagents and Equipments TRIzol Reagent (Life Technologies cat# 15596-026) or TRI reagent (Sigma cat # T-9424) DEPC (RNase free) water or 0.5% SDS solution in DEPC treated water Chloroform (Fishe ...

1.RNAi： 近年来的研究表明一些小的双链RNA可以高效、特异的阻断体内特定基因表达，促使mRNA降解，诱使细胞表现出特定基因缺失的表型，称为RNA干扰（RNA interference，RNAi，也译作RNA干预或者干涉）。它也是体内抵御外在感染的一种重要保护机制。 2.siRNA： siRNA（small interfering RNAs）是一种短片断双链RNA分子，能够以同 ...

RNA一度被认为仅仅是DNA和蛋白质之间的“过渡”，但越来越多的证据清楚的表明，RNA在生命的进程中扮演的角色远比RNA我们早前设想的更为重要。RNA干扰（RNA interference）的发现使得人们对RNA调控基因表达的功能有了全新的认识，更因为可以简化替代基因敲除而成为研究基因功能的有力工具，因此格外引人注意，在2002年度Science评选的10大科 ...

将制备好的siRNA，siRNA表达载体或表达框架转导至真核细胞 中的方法主要有以下几种： 1.磷酸钙共沉淀 将氯化钙，RNA(或DNA )和磷酸缓冲液混合，沉淀形成包含DNA 且极小的不溶的磷酸钙颗粒。磷酸钙-DNA 复合物粘附到细胞 膜并通过胞饮进入目的细胞 的细胞 质。沉淀物的大小和质量对于磷酸钙转染的成功至关重要。在实验中使用的每种试剂都必须小心校准，保证质量，因为甚至偏离最优条件十分之 ...