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Transcription of cRNA probe Reagents/Solutions DNA (PCR or plasmid) with bacteriophage RNA polymerase promoter in suitable orientation (see note 1 ) ~1µg/µl in TE buffer 10mM MgCl2 5x Tr ...

The ribonuclease protection assay (RPA) is a highly sensitive and specific method for the detection of mRNA species. The assay was made possible by the discovery and characterization of DNA-dependant ...

Secondary structure of prion mRNA Luck R; Steger G; Riesner D Institut fur Physikalische Biologie Heinrich-Heine-Universitat Dusseldorf FRG. J Mol Biol 258: 813-26 (1996) An algorithm for prediction ...

All solutions used in RNA preparation should be treated with DEPC as follows: add DEPC as follows: add DEPC to 0.1% and vigorously stir for one hour to O/N followed by autoclaving for 30 minutes ...

RNAi相关文章（for free） Prospects of RNA interference therapy for cancer S IPai Y-YLin BMacaes AMeneshian C-FHung and T-CWu Gene Ther 13: 464-477; ...

(1) Core out a single well separated plaque from the agar plate using a 200µl pipette tip and place it into 500µl of SM buffer in a 1.5ml microtube: SM buffer 5.8g of NaCl 2.0g of M ...

Using siRNA for gene silencing is a rapidly evolving tool in molecular biology. There are several methods for preparing siRNA such as chemical synthesis in vitro transcription siRNA expression vectors ...

We use the Promega Ribomax Large Scale RNA Production System T7 (Cat. No.: P1300) to produce dsRNAs and the RNAi Exp.html" target=_blank> Jack Dixon protocol ( RNAi _Dixon.html" target=_b ...

In the past poly(A)+ RNA has not been detected in prokaryotes. In the early 80s a new method of isolating RNA from bacteria was developed involving lysis by protease K in the presence of SDS and ...

Background Information of RNA i RNA interference (RNA i) is a biological process in which the introduction of double-stranded RNA (dsRNA ) into a cell results in targeted post-tranional gene silencing ...

RNA for S1 or PE analysis must be free of chromosomal DNA. This can be accomplished by extraction of the RNA with hot phenol but phenol can especially if its pH is acidic cause the specific loss ...

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A modification of the basic immunofluorescence staining and flow cytometric analysis protocol can be used for the simultaneous analysis of surface molecules and intracellular cytokines at single-cell ...

1. Snap freeze ~107 cells or ~100 mg of tissue in liquid nitrogen or dry ice/ethanol. 2. Transfer the frozen sample to a mortar and pestle and grind to a fine powder. Or use a hand-held tissue grinder ...

In vitro transcription with yeast nuclear extract Steve Hahn Last Modified Fri Apr 25 2003 Wear gloves throughout use RNAse free solutions (either autoclaved or sterile filtered) and clean bench and ...

siRNA Design RNAi target selection rules : Targeted regions on the cDNA sequence of a targeted gene should be located 50-100 nt downstream of the start codon (ATG). Search for sequence motif AA ...

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000" height="300" width="500" classid="clsid:D27CDB6E-AE6D-11cf-96B8-444553540000" How does RNAi work? Genetic and biochemical data indicate a possible two-step mechanism for RNA i ...

Reagents and Equipments TRIzol Reagent (Life Technologies cat# 15596-026) or TRI reagent (Sigma cat # T-9424) DEPC (RNase free) water or 0.5% SDS solution in DEPC treated water Chloroform (Fishe ...

1.RNAi： 近年来的研究表明一些小的双链RNA可以高效、特异的阻断体内特定基因表达，促使mRNA降解，诱使细胞表现出特定基因缺失的表型，称为RNA干扰（RNA interference，RNAi，也译作RNA干预或者干涉）。它也是体内抵御外在感染的一种重要保护机制。 2.siRNA： siRNA（small interfering RNAs）是一种短片断双链RNA分子，能够以同 ...