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A practical method for the preparation of total DNA from filamentous fungi M.I. Borges M.O. Azevedo R. Bonatelli Jr. M.S.S. Felipe and S. Astolfi-Filho Departamento de Biologia Celular Universidade de ...

Electroelution of agarose fragments Electroelution buffer 1 M Tris pH 7.5 12.0 mls 0.5 M EDTA 0.24 mls 1 M NaCl 3.0 mls qs to 600 mls dH2O Acetate cushion 3 M NaAcetate pH 4.8 480 μl 0.1 % Bromphe ...

I have tried to purify my PCR products by using 3 different approaches: 1) QIAQuick PCR purification kit: but when I run the gel to see its efficacy there was simply nothing!! it cleaned everything in ...

Kitto Lab The University of Texas at Austin http://research.cm.utexas.edu/bkitto/Kittolabpage/Protocols/Microbiology/electroporation.html This procedure prepares glycerol stock cultures of bacteria f ...

Kitto Lab The University of Texas at Austin http://research.cm.utexas.edu/bkitto/Kittolabpage/Protocols/Microbiology/ethanolPpt.html This procedure allows the concentration of DNA samples from dilute ...

Mitochondrial DNA Isolation from Somatic Embryogenic Cell Cultures of Larix (Excerpt from Thesis). Mitochondrial DNA is isolated by a modification of the methods described by Wilson and Chourey (1984) ...

Preparation of Sonicated Salmon Sperm DNA Procedure: 1) Use Pharmacia #27-4564-01. With clean flamed scissors and forceps weigh 0.25 g/50 ml conical and add 50 ml/conical of 0.02 M Tris pH 7.6. Allo ...

The Preuss LabThe Division of Biological SciencesThe University of Chicago http://preuss.bsd.uchicago.edu/protocols/topo.html ...

Contributed by Dr. A. Gratchev Single Nucleotide Primer Extension is a powerful method which can be used for the precise analysis of methylation in a certain position. The procedure is shown on the fi ...

Hi all! We are trying to extract genomic DNA from the leaves of an Ericacea (we suppose it is rich in tanins and phenolics) and we can't do it! We have used two different kits which work OK for other ...

Source: Protocol Online Abstract: Modifying DNA using sodium bisulfite to convert unmethylated cytosines to uracils and subsequently detect methylated cytosines using methylation specific PCR (MSP) te ...

1. Transfer plugs of mycelial margin to 0.1 P plates. Allow to grow to fill plate (about one week at 21°). 2. Macerate two plates of mycelia in a blender with 50 ml of .002 P liquid medium. Inocul ...

- make a 5µm section to do an evaluation of the % tumour cells - make 50X50µm sections for the DNA isolation - put sections in a falcon tube containing 10 ml of 1XSE - add 100µg/ml p ...

1.Vectorpedia： 输入质粒骨架，直接查询，非常方便; http://www.addgene.org/pgvec1?f=v&cmd=listvecinfo 2. google 检索式子：质粒名 ＋ map 质粒名 ＋sequence ＋filetype:txt or filetype:pdf 或者：进入google，点击"图片搜索"，直接查找图谱。 3.go ...

LiAc/SS-DNA/PEG TRANSFORMATION TRAFO Efficiency: up to 22 million transformanst/ µg of plasmid DNA HIGH EFFICIENCY TRANSFORMATION Please cite: Agatep R. R.D. Kirkpatrick D.L. Parchaliuk R ...

Preparation of plasmid DNA : a modified mini alkaline-lysis/PEG precipitation procedure. ABI; 1995 Materials GTE buffer (50mM glucose 25mM Tris-HCl (pH8.0) 10mM EDTA (pH 8.0)) (200µl/tube) 0.2N ...

Abstract: Single strand conformational polymorphism (SSCP)is the most widely used PCR-based methods for point mutation detection. The abnormal band found by SSCP analysis is normally verified ...

Tissue collection storage microdissection sectionin g: See separate protocol. Tissue handling : Note that all fresh tissue shoμld be handled as BioSafety Level 2 materials (wear gloves lab coat etc ...

1）致癌化学物转化细胞：转化叙利亚地鼠胚胎细胞实验 1．取材：妊娠后第13天取材，取数个同窝胚胎，去头和内脏，在无菌条件下剪碎至米粒大小，再用0.25%胰蛋白酶（含0.01%EDTA）37℃消化30分钟，滤过、低速离心、吸除消化液、加入营养液、制备成细胞悬液、接种入75cm/培养瓶中，接种密度10～20×106/75cm，37℃培养2～3天，在顺利情况下能迅速长满瓶面。 2．贮存：选大 ...

双酶切buffer的选择： 1、U ：Supplied with its own unique reaction buffer that is different from the four standard NEBuffers. Its compatibility with the four standard NEBuffers is indicated by the chart. 2、BSA ...