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Procedure for 100 ~ 300 mg of tissue. 1. Collect fresh tissue coleoptiles or small amount of leaf tissue. Use about three inches of a leaf blade. Preferably younger tissue. Fold leaf and place i ...

I am working with birds of prey....i am looking for a simple method for isolating genomic DNA from avian breast muscle....I have found protocols for mammalian tissue...will these work fine? als ...

Localization of particular sequences within genomic DNA is usually accomplished by the transfer techniques described by Southern (1975). Genomic DNA is digested with one or more restriction enzymes an ...

Materials: Whatman 3 mm Blotting Paper nitrocellulose (Schleicher & Schuell Amersham) or nylon membrane filter (Amersham). Paper towels (preferably C-fold "cheap-o" variety) Pyrex or Tup ...

Hahn Lab，The Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute http://www.fhcrc.org/science/labs/hahn/methods/genetic_meth/yeast_transf_rapid.html ...

Sequence Analysis . Automated (Semi-Automated) Sequencers generate a four-color chromatograms on the results of the sequencing gel as well as a text file of sequence data. The sequencer can not verif ...

CTAB TECHNIQUE / Method / Schedule / Protocol (JPB) FOR DNA ISOLATION / DNA EXTRACTION FROM PLANT LEAF / LEAVES SAMPLES (see also if both DNA and RNA are needed) Reagents needed CTAB buffer 2% CTAB&nb ...

Molecularpourous membrane tubing is used for desalting protein and DNA isolation / purification. It comes in several diameters and pore sizes (for molecular weight conversions for nucleic acids see ge ...

1. Separate DNA fragments in an agarose gel cast with 0.5 mg/mL Ethidium bromide. Locate bands with a hand-held long-wave UV lamp.2. Slice the gel with a razor blade above and below the bands of inter ...

What is a crystaline precipitate that I get sometimes (not always) when I precipitate my genomic DNA preps and how can I get rid of it? theories are protein(that was not completely removed durin ...

Phenol-based Method for the Isolation of DNA Fragments from Low-Melting Temperature Agarose Reference: Favre D. 1992. Biotechniques vol. 13 1.Cut out slice containing DNA smallest size possible. 2.E ...

1.Inoculate a couple of 20 ml LB broth in a 125 ml flasks with 0.5 ml of an overnight culture of the recipient strain. 2.Shake at 37℃ until O.D. 600=0.13 - 0.15 (1 - 2 hours). Measure by taking ...

Buffer TfbI pH5.8 500ml1.47g KOAC ...

Day 1 1. Digest DNA for 6 hours (or overnight) BSA 10 mg/ml 0.5 22.65 μl of 10 μg Genomic DNA RnaseA 10mg/ml 0.1 in TE mixed to 7.35 μl cocktail Spermidine 100nM 0.75 per sample 10X enzyme b ...

NOTE: THIS IS FINE FOR SOUTHERNS BUT NOT FOR SCREENING BY PCR. (from Ruixia 7/99 from protocol by Stef Oehen 7/94). 1. Put 1 cm tail in 1.5 ml microcentrifuge tube. 2. Add 500µl Tail Buffer an ...

This protocol originated in Murray and Thompson (1980) and then was modified by Richard Jorgensen and finally published in Wagner et. al 1987. 1.Start with about 10 grams fresh needles. 2.Chop into s ...

载体：携带外源DNA 进入宿主细胞的工具。 一、载体的功能 1.运送外源基因高效转入受体细胞。 2.为外源基因提供复制能力或整合能力。 3.为外源基因的扩增或表达提供条件。 二、载体应具备的条件 1.具有对受体细胞的可转移性。 2.具有与特定受体细胞相适应的复制位点或整合位点。 3.长度尽可能小，以提高其载装能力。 4.具有多种单一的酶切位点。 5.具有合适的选择性标记。 附图： ...

Uniplex PCR-based Sequencing Latest update 2-21-00 A.PCR Reactions: B.Processing PCR products: C.Alternative PCR Reaction Cleanup steps instead of passage through Sephadex G-50 usually not performe ...

Two protocols are given here. The first one is used for BAC library screening on filters although is also suitable for Transfer hybridizations. PREPARATION OF HYBRIDIZATION SOLUTION Prehybridizati ...

Hi there Is anyone know how short of the CpG island can be? let say if the CG sequence of about 30bp is that possible the short CpG sequence be methylated? Thanks you! Pine -pine- ------------------- ...