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Does somebody know how to increase DNA concentration from a too weakly concentrated DNA sample obtained with a Qiagen Extraction Kit (the elution buffer was ddH2O) ?? -BATY- ------------------------- ...

This procedure isolates DNA from agarose gels by filtration through a filter-paper column. The column is made in a 500 μl tube from a slurry of filter paper in TE buffer. Materials Whatman 3MM fil ...

1、用合适的酶切割DNA并电泳。 2、于紫外灯下从凝胶上切下所要的DNA带，装入含1×TBE缓冲液的透析代内，用夹子封好袋口，并检查有无漏孔。 3、将透析袋（纵长）与两极间的边线垂直放于电泳槽内电泳，4～5V/cm电泳至DNA贴紧袋壁。 4、取出透析袋，挤出凝胶块，吸出袋内液体放入1.5ml的离心管内，10000rpm离心5min。 5、吸出上清放入离心管内，加入等体积的饱和酚和等体积 ...

About 1/2 to 1 cm of tail should be cut from properly marked mice (using toe or ear clipping etc) about the age of 2-3 weeks. Place these tails into marked 1.5 ml Eppindorf tubes for processing; they ...

I recovered DNA from gel by Millipore's kitIt's simple.Cut the band from gel and centrifuge for 10 minutesbut the efficiency is disappointedonly 20% or so. Can anyone reccomend me other methods?Thanks ...

For fragments from 200 bp to 10 kb the agarose purification is ideal. For smaller fragments (20 bp to 400 bp) the acrylamide purification is preferred. Solutions Crush and Soak Solution 500 mM NH4 OAc ...

Hahn Lab，The Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute http://www.fhcrc.org/science/labs/hahn/methods/mol_bio_meth/BigDye Protocol.pdf ...

Preparation of C. elegans Genomic DNA Protocol by Andy Fires Lab Modified my Min-Ho Lee and Sudhir Nayak This protocol is based on the original Fire lab protocol for isolating genomic DNA from C. eleg ...

Important Notes Before Starting • New users are strongly recommended to read the QIAGEN handbook before starting the procedure. •Before using the kit for the first time dissolve the lyophi ...

Hi Dear All Have you ever tried to ligate 3 DNA fragments with sticky ends into Vector(5.2kb). A. Fragment: 3.0kb with HindIII/ Bam HI cut from a Vector. B. Fragment: 0.5kb BamHI / XbaI cut fro ...

Isolation of Mouse genomic DNA Kill mouse by cervical luxation and dissect liver. Add to a 50ml tube and place on liquid nitrogen. Grind to a powder under liquid nitrogen using a mortar and pe ...

Protocol for Sequencing Very Difficult Regions Ziyun Yao and B.A. Roe 02-14-2002 Target DNA (SAP-ExoI cleaned PCR 7-deaza-dG containing product) ...

Hi Does enzyme digestion before bisulfite treatment make a big difference - i am having a few problems with incomplete conversion. If so can anyone reccomend me a good 'general' enzyme to use - I know ...

Prior to getting cells: 1) Turn on 42 deg bath. Takes about 30 min to reach 42 deg. 2) Put 0.1 M sterile CaCl2 on ice. 3) One tube of cells is good for several transformations. For two transform ...

I need to purify 20mer from a mixture containing 200mer and 20mer. How to purify this small fragment? Thanks~~ -HS- -------------------------------------------------------------------------------- Hi ...

Southern Blotting: Detection via Chemiluminescence (BM's Genius System) Modified to include the use of CSPD for chemiluminescent detection! This is the method we are currently using for Southern blo ...

I'm trying to do a southern blot and I have a trouble the bands I get are the same bands that appear in the genomic DNA digestion my probe is specific and it works in northern blot. Thanks -PPlopez- - ...

Hi I am a Biochem student.I am extracting DNA from plants that have been exposed to contaminants then using restriction enzymes to see if there is any change.I was wondering if there was a ...

Principle The nucleic acids contained within the gel slice are electrophoresed out of the gel funneled down into the v-shaped channel and trapped within a high salt cushion resting at the bottom of th ...

Hi. I am a new member of this forum. Currently I am trying to look for some novel methylated gene in NPC cell line. What method should I start with? Pls help. Thank you. -CHOO- ---------------------- ...