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Cepko/Tabin Lab Harvard University http://axon.med.harvard.edu/~cepko/protocol/mike/E1.html Cepko/Tabin Lab Harvard University http://axon.med.harvard.edu/~cepko/protocol/mike/E1.html Solutions 10X A ...

when I extracted DNA from chicken red blood cellI met a very strange question.After the phenol and chloroform treation.I collected the upper layers (it is not liquid but like some transparent s ...

The Minion Lab College of Veterinary Medicine at Iowa State University http://mycoplasmas.vm.iastate.edu/lab_site/methods/DNA /restrdig.html ...

Overview Procedure 1. Preheat the CTAB Isolation Buffer at 60℃. 2. Grind 2 g of fresh leaf tissue to a powder in Liquid Nitrogen in a chilled mortar and pestle. 3. Scrape the powder into a chilled 50 ...

Prepare: 200ml LB in a 1L flask 1L sterile ddH2O and place in cold room 4 50ml conicals chilled on ice 10% glycerol (cold) Chilled boxes of 100μl and 1ml pipet tips. Chilled 0.5ml tubes. General Th ...

RNA/Zeta Probe Dot Blotting protocol 1)Make up RNA (up to 20μg)dissolved in sterile H2 OTE or 0.5% SDS to 500μl with ice-cold sterile 10mM NaOH1mM EDTA and apply it to Zeta Probe membraneheld in ...

长时间反应时内切酶的活性保持情况因酶而异。本表中列出了在16小时内完全酶解底物DNA所需的最小酶量。 实验方法：在50µl的反应体系中，分别加入1µg的单位定义底物DNA和1.00、0.50、0.25和0.13个单位的内切酶，37℃（或要求的最适温度）反应16小时。用琼脂糖凝胶电泳法来确定在16小时内酶解1µg底物DNA所需的最小酶量。 16小时酶 ...

Retroelements and their derivatives are a ubiquitous and abundant component of plant genomes. From the 1990s PCR based techniques have been developed to isolate the elements from genomic DNA of differ ...

Plasmid DNA isolated by this procedure can be used routinely for electrophoretic analysis restriction endonuclease digestion and transformation of E. Coli. DNA sequencing PCR and most other molecular ...

The following protocol is based on our modifications of R. Kraft J. Tardiff K. S. Krauter and L. A. Leinwand. Biotechn . 6(6):544-545 1988. 1.Inoculate 2-5 ml of L broth containing the appropriate ant ...

1.Wash the plates briefly using the 96-channel block washer. 2.Place the plates in a tub submerged in dd-water. 3.Put a flask on top of the plates to keep them submerged. 4.Place the tub with the s ...

Hi.- I have a problem with amplification of DNA by PCR. The extraction of DNA is direct from plant. i use PVP for keep out of DNA Polyphenols from the samples. The problem is: i can't view apmlificati ...

本方法通过SDS裂解细胞，蛋白酶降解蛋白，CTAB去除多糖成分 一:仪器:同方法一 二:试剂: TE、TAE缓冲液;10%SDS;5MNaCL; 20mg/ml蛋白酶K;CTAB/NaCL溶液(10% CTAB，0.7M NaCL 4.1gNaCL 溶于80ml水中，缓慢加入10gCTAB，加热溶解);25/24/1，酚/氯仿/异戊醇; 24/1，氯仿/异戊醇;异丙醇;70%及100 ...

Preparation of Silica 1. Suspend 5 g of silica (Sigma S-5631) in 50 ml of PBS. 2. Allow the silica to settle for 2h. 3. Discard the supernatant containing fine particulate matter. 4. Repeat steps 2 an ...

Sugden labMcArdle Laboratory for Cancer Research University of Wisconsin-Madison Medical School http://mcardle.oncology.wisc.edu/sugden/Protocols/html files/Filter Binding Assay.htm Last Modified 5.2. ...

(adapted from Bruce A. Roe Department of Chemistry and Biochemistry The University of Oklahoma Norman Oklahoma 73019 broe@ou.edu) A. Sonication The generation of DNA fragments by sonication is perfor ...

Does anybody know how to isolate single-stranded DNA from mammalian cells? Thanks for responding. Yin -- -------------------------------------------------------------------------------- Hello I don't ...

An U ...

1.Remove gel slice contain DNA fragment and place in 10 volumes of: 300 mM NaOAcpH 7.0 300 ml 1 M NaOACpH 7.0 1 mM EDTA 2 ml 500 mM EDTApH 8.0 698 ml ddH20 2.Incubate at 22℃ for 30 min.Transfer gel sl ...

Non-radioactive Probes I. Via random hexamers 1. Solutions: 10X hexa nt miundefined: 500 mM Tris-Cl pH 7.2 100 mM MgCl2 1 mM dithioerythritol (DTE) 2 mg/ml BSA 62.5 A260 units/ml (1.56 mg/ml) random hexan ...